A mesenchymal stem cell for inhibiting tumor growth, preparation method and application thereof

A technology of mesenchymal stem cells and tumors, which is applied in the field of mesenchymal stem cells and preparations that inhibit tumor growth, and can solve problems such as limitations
CN108715832BActive Publication Date: 2020-11-10北京华奥玄德生物医药科技有限公司
8 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
北京华奥玄德生物医药科技有限公司
Publication Date
2020-11-10

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a mesenchymal stem cell for inhibiting tumor growth and a preparation method and an application, and relates to the technical field of the medicine. The provided mesenchymal stem cell for inhibiting the tumor growth is a TLR5 positive mesenchymal stem cell. The preparation method for the TLR5 positive mesenchymal stem cell is to separate TLR5 high-expression positive mesenchymal stem cells by using a flow-type or overexpress TLR5 on the mesenchymal stem cell. The invention further provides a cultural supernatant containing a serum-free medium and the TLR5 positive mesenchymal stem cell, and further provides applications of the TLR5 positive mesenchymal stem cell and the cultural supernatant for preparing an anti-tumor drug. The cultural supernatant in TLR5+MSC conditions has the remarkable inhibiting effects to the in-vitro proliferation, the migration and the clonality of a tumor cell. The TLR5-activated MSC is capable of remarkably improving the in-vitro killing activity of a NK cell. The TLR5-activated MSC is capable of inhibiting the tumor growth in Lewis lung cancer mice.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of medicine, and in particular relates to a mesenchymal stem cell for inhibiting tumor growth, a preparation method and application thereof. Background technique

[0002] Tumor is one of the major diseases leading to death in the world. The tumor microenvironment plays an important role in the occurrence, development, invasion and metastasis of tumors. Mesenchymal stem cells (MSCs) can participate in the immune regulation of the tumor microenvironment in vivo, and have a close relationship with tumors. The microenvironment is closely related. On the one hand, tumor tissue secretes a large number of inflammatory factors and chemokines during the growth process, recruiting MSCs to homing to the tumor site; on the other hand, active substances such as IDO, PGE2, TGF-β1, and MMP-9 secreted by MSCs It can act on tumor cells and immune cells to affect the growth of tumors. MSC has the function of repairing the b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More