siRNA capable of inhibiting RBM3 gene expression of human melanoma and plasmid vector of siRNA

A melanoma, gene expression technology, applied in the field of genetic engineering, can solve the problem that there is no RNAi technology and affect the proliferation ability of melanoma cells, and achieve the effect of inhibiting proliferation in vitro

Inactive Publication Date: 2017-08-18
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether the oncogene RBM3 affects the proliferation ability of melanoma cells is still unclear, and there is no RNAi technology specifically for inhibiting the expression of human melanoma RBM3 gene

Method used

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  • siRNA capable of inhibiting RBM3 gene expression of human melanoma and plasmid vector of siRNA
  • siRNA capable of inhibiting RBM3 gene expression of human melanoma and plasmid vector of siRNA
  • siRNA capable of inhibiting RBM3 gene expression of human melanoma and plasmid vector of siRNA

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Effect test

Embodiment 1

[0034] An siRNA for inhibiting the expression of human melanoma RBM3 gene, said siRNA can inhibit the expression of human melanoma RBM3 gene, and the sense chain sequence is the sequence shown in SEQ NO.1.

[0035]A plasmid carrier of siRNA that inhibits the expression of human melanoma RBM3 gene, the plasmid vector contains the siRNA capable of inhibiting the expression of human RBM3 gene; the positive-sense strand sequence of the siRNA is the sequence shown in SEQ NO.1.

[0036] A method for constructing a plasmid vector of siRNA that suppresses human melanoma RBM3 gene expression, characterized in that: the method for constructing comprises the following steps:

[0037] 1) Synthesis of DNA sequence corresponding to siRNA

[0038] According to the positive-sense strand sequence SEQ NO.1 of the siRNA in the sequence table, design its corresponding DNA sequence, introduce restriction sites at both ends of the sequence, the DNA sequence is two single strands, and the two single...

Embodiment 2

[0047] An siRNA for inhibiting the expression of human melanoma RBM3 gene, said siRNA can inhibit the expression of human melanoma RBM3 gene, and the sense chain sequence is the sequence shown in SEQ NO.5.

[0048] A plasmid vector of siRNA that inhibits the expression of human melanoma RBM3 gene, the plasmid vector contains the siRNA capable of inhibiting the expression of human RBM3 gene; the positive-sense strand sequence of the siRNA is the sequence shown in SEQ NO.5.

[0049] A method for constructing a plasmid vector of siRNA that suppresses human melanoma RBM3 gene expression, characterized in that: the method for constructing comprises the following steps:

[0050] 1) Synthesis of DNA sequence corresponding to siRNA

[0051] According to the positive-sense strand sequence SEQ NO.5 of the siRNA in the sequence table, design its corresponding DNA sequence, introduce restriction sites at both ends of the sequence, the DNA sequence is two single strands, and the two single...

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Abstract

The invention discloses siRNA capable of inhibiting RBM3 gene expression of human melanoma as well as an expression vector and an application of the siRNA, and belongs to the technical field of genetic engineering. According to the invention, five siRNAs, which can specifically silence RBM3 gene expression of human melanoma cells, are disclosed; and the invention provides the vector capable of expressing and generating the siRNA and a constructing method of the vector, as well as the application of the siRNA capable of inhibiting RBM3 gene expression of the human melanoma and the expression vector of the siRNA in aspect of inhibiting in vitro proliferation of the melanoma. With the application of the siRNA which can inhibit the RBM3 gene expression in the human melanoma cells provided by the invention, the in vitro proliferation of the human melanoma cells can be effectively inhibited; and an effect of RBM3 gene on promoting proliferation of the melanoma cells is proved for the first time, so that a novel target is provided for the treatment of the melanoma, and a certain theoretical basis and a feasible medicine are offered for the treatment of the melanoma.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to an siRNA and a plasmid carrier thereof for inhibiting the expression of human melanoma RBM3 gene. Background technique [0002] Melanoma, also known as malignant melanoma, is a type of malignant tumor derived from melanocytes. It is commonly found in the skin, and also in mucous membranes, eye choroid and other parts. It is a malignant skin tumor arising from the abnormal proliferation of melanocytes derived from the neural crest, which is highly metastatic and resistant to general chemotherapy. Therefore, although the incidence of melanoma only ranks third among skin cancers, its fatality rate is the highest. Among all skin cancer-related deaths, nearly 80% of patients die from melanoma. Melanoma is the most malignant type of skin tumors, effectively blocking the proliferation of melanoma cells can provide great help for the treatment of melanoma. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/85C12N15/66A61K48/00A61K31/7088A61P35/00
CPCC12N15/1135C12N15/66C12N15/85C12N2310/14
Inventor 杨海杰王勉王磊程彬峰高姗姗
Owner XINXIANG MEDICAL UNIV
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