siRNA capable of inhibiting RBM3 gene expression of human melanoma and plasmid vector of siRNA
A melanoma, gene expression technology, applied in the field of genetic engineering, can solve the problem that there is no RNAi technology and affect the proliferation ability of melanoma cells, and achieve the effect of inhibiting proliferation in vitro
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Embodiment 1
[0034] An siRNA for inhibiting the expression of human melanoma RBM3 gene, said siRNA can inhibit the expression of human melanoma RBM3 gene, and the sense chain sequence is the sequence shown in SEQ NO.1.
[0035]A plasmid carrier of siRNA that inhibits the expression of human melanoma RBM3 gene, the plasmid vector contains the siRNA capable of inhibiting the expression of human RBM3 gene; the positive-sense strand sequence of the siRNA is the sequence shown in SEQ NO.1.
[0036] A method for constructing a plasmid vector of siRNA that suppresses human melanoma RBM3 gene expression, characterized in that: the method for constructing comprises the following steps:
[0037] 1) Synthesis of DNA sequence corresponding to siRNA
[0038] According to the positive-sense strand sequence SEQ NO.1 of the siRNA in the sequence table, design its corresponding DNA sequence, introduce restriction sites at both ends of the sequence, the DNA sequence is two single strands, and the two single...
Embodiment 2
[0047] An siRNA for inhibiting the expression of human melanoma RBM3 gene, said siRNA can inhibit the expression of human melanoma RBM3 gene, and the sense chain sequence is the sequence shown in SEQ NO.5.
[0048] A plasmid vector of siRNA that inhibits the expression of human melanoma RBM3 gene, the plasmid vector contains the siRNA capable of inhibiting the expression of human RBM3 gene; the positive-sense strand sequence of the siRNA is the sequence shown in SEQ NO.5.
[0049] A method for constructing a plasmid vector of siRNA that suppresses human melanoma RBM3 gene expression, characterized in that: the method for constructing comprises the following steps:
[0050] 1) Synthesis of DNA sequence corresponding to siRNA
[0051] According to the positive-sense strand sequence SEQ NO.5 of the siRNA in the sequence table, design its corresponding DNA sequence, introduce restriction sites at both ends of the sequence, the DNA sequence is two single strands, and the two single...
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