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Microalgae sulfated complex polysaccharide and its preparation method and application

A technology of microalgae sulfate and compound polysaccharide, which is applied in pharmaceutical formulations, drug combinations, medical preparations containing active ingredients, etc. effect of growth

Active Publication Date: 2021-02-26
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] Through the search, no research has been found on the combination of spirulina and chlorella for anti-tumor activity

Method used

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  • Microalgae sulfated complex polysaccharide and its preparation method and application
  • Microalgae sulfated complex polysaccharide and its preparation method and application
  • Microalgae sulfated complex polysaccharide and its preparation method and application

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Embodiment Construction

[0050] The present invention will be further described in detail below in conjunction with the accompanying drawings and through specific embodiments. The following embodiments are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0051] 1. Preparation of chlorella polysaccharide (CSP) and spirulina polysaccharide (PSP)

[0052] (1) Wall breaking of Spirulina or Chlorella cells: Centrifuge the Spirulina or Chlorella liquid that has grown to the plateau stage, weigh 10g of algae powder, add 90mL of distilled water, and freeze the algae liquid at -20°C overnight Agglomerate, then heat in a water bath for 8 hours to thaw and break the wall, repeat the above process 3 times, centrifuge at 4500r / min for 10 minutes, take the supernatant, shake the algae mud, add distilled water and then ultrasonically break the wall: 600-800W, 1 minute each time, 10 minutes in total After completion, centrifuge at 4500r / min for 10min to take the sup...

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Abstract

The invention relates to a microalgae sulfated composite polysaccharide, which is composed of sulfated chlorella polysaccharide: sulfated spirulina polysaccharide in a ratio of 1:16-16:1. The microalgae sulfated compound polysaccharide of the present invention can effectively inhibit the proliferation of tumor cells in vitro, and because it has good water solubility, it can play a role in vivo and can well inhibit the growth of tumors.

Description

technical field [0001] The invention belongs to the medical application field of marine organisms, and relates to spirulina and chlorella, in particular to a microalgae sulfated composite polysaccharide, a preparation method and application thereof. Background technique [0002] The growth conditions and environmental characteristics of microalgae determine that the intracellular polysaccharides of microalgae have some structures and functions that are different from other biological polysaccharides. The application potential of such polysaccharides in the field of medicine and medicine has attracted more and more people's research interest in them. However, a large number of studies in recent years have found that microalgal polysaccharides from different sources have different biological activities, and the biological activity of a single polysaccharide cannot comprehensively improve the immunity of the human body. [0003] Spirulina (Spirulina platensis) is a class of low...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
Inventor 宋东辉
Owner TIANJIN UNIV OF SCI & TECH
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