Green algae polysaccharide and preparation method thereof
A technology of chlorophyll polysaccharides and sulfated polysaccharides, applied in the field of marine biological polysaccharides, can solve the problems of chlorophyll polysaccharide structure complexity, inhomogeneity, and restrictions on in-depth research, and achieve obvious anticoagulant activity, no toxic side effects, and good market application foreground effect
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[0053] The present invention also provides a preparation method of Chlorella polysaccharide, the method includes the following steps:
[0054] (1) Add water to the dried bag reef film to form a homogenate, extract at 20°C-24°C for 0.5-4 hours, and separate the clear liquid; where the quality of the water is 10-60 times the quality of the bag reef film;
[0055] (2) Concentrating and desalting the obtained clear liquid under reduced pressure to obtain a first polysaccharide solution; concentrating and drying the first polysaccharide solution under reduced pressure to obtain a polysaccharide; dissolving the polysaccharide in water to obtain a second polysaccharide solution; The mass fraction of the polysaccharide in the second polysaccharide solution is 0.1%-6%;
[0056] (3) Separating the second polysaccharide solution through an ion exchange chromatography column and a gel chromatography column, and then concentrating and desalting under reduced pressure, and concentrating and drying...
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[0062] Example 1
[0063] (1) Add 1500 grams of water to 50 grams of dry bag reef film to form a homogenate, stir and extract at 21°C for 3.0 hours, centrifuge to discard the precipitate, and separate the clear liquid;
[0064] (2) Concentrate the resulting clear liquid under reduced pressure and desalinate through dialysis to obtain the first polysaccharide solution. The step of dialysis desalination includes: placing the clear liquid after concentration under reduced pressure in a dialysis bag with a molecular weight cut-off of 3500 Dalton for 3 days. , Stop dialysis when the conductivity meter tests the external fluid after dialysis to a constant value;
[0065] The first polysaccharide solution was concentrated under reduced pressure, and 95% ethanol was added to the concentrated first polysaccharide solution to precipitate the polysaccharide. The volume of ethanol was four times the volume of the first polysaccharide solution, and the resulting precipitate was kept at 40°C Dry ...
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