Multipurpose functional water and preparation method thereof
A functional water and multi-purpose technology, which can be applied in cosmetic preparations, chemical instruments and methods, pharmaceutical formulas, etc., can solve the problems of low extraction rate of active ingredients, unfavorable industrial application, and waste of raw materials, so as to shorten extraction time and enhance Skin elasticity, the effect of increasing the extraction rate
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[0026] Preparation Example 1
[0027] Step 1: Prepare the Leech Extract
[0028] 1000g of leeches were soaked in distilled water for 12 hours, and the soaked leeches were minced with a meat grinder to obtain a leech homogenate; 2) 15 times the volume of 4% NaCl solution was added to the leech homogenate to obtain a leech solution; 3 ) The leech solution was ultrasonically treated at 60 kHz for 10 minutes to fully separate the active components of the leech; 4) After 5 hours, the filtrate was obtained by filtration, and ammonium sulfate was added to the filtrate, and the filtrate was salted out for 8 hours. The salting-out temperature was controlled at 0-2 °C, and the precipitate was collected. 5) Add distilled water to the collected precipitate to dissolve and transfer to a dialysis bag, and dialyze at 0°C for 48h to obtain 10L of leech extract.
[0029] The main active ingredient in the leech extract is hirudin, and measuring the protein content of the extract can indicate t...
Example
[0051] Preparation Example 2
[0052] Step 1: Prepare the Leech Extract
[0053] 1000g of leeches were soaked in distilled water for 24 hours, and the soaked leeches were minced with a meat grinder to obtain a leech homogenate; 2) 20 times the volume of 4% NaCl solution was added to the leech homogenate to obtain a leech solution; 3 ) The leech solution was ultrasonically treated at 200 kHz for 30 minutes to fully separate the active components of the leech; 4) After 10 hours, the filtrate was obtained by filtration, and ammonium sulfate was added to the filtrate, salting out for 10 hours, and the salting-out temperature was controlled at 0-2 °C, and the precipitate was collected. 5) Add distilled water to the collected precipitate to dissolve and transfer to a dialysis bag, and dialyze at 0°C for 48h to obtain 10L of leech extract. The protein content determined was 1.5 mg / ml.
[0054] Step 2: Preparing the Multi-Mineral Element Solution
[0055] 1) Pulverize celestite, me...
Example
[0063] Preparation Example 3
[0064] Step 1: Prepare the Leech Extract
[0065] 1000g of leeches were soaked in distilled water for 20 hours, and the soaked leeches were minced with a meat grinder to obtain a leech homogenate; 2) 18 times the volume of 4% NaCl solution was added to the leech homogenate to obtain a leech solution; 3 ) The leech solution was ultrasonically treated at 100 kHz for 20 minutes to fully separate the active components of the leech; 4) After 9 hours, the filtrate was obtained by filtration, and ammonium sulfate was added to the filtrate, salting out for 9 hours, the salting-out temperature was controlled at 0-2 °C, and the precipitate was collected. 5) Add distilled water to the collected precipitate to dissolve and transfer to a dialysis bag, and dialyze at 0°C for 48h to obtain 10L of leech extract. The protein content determined was 1.8 mg / ml.
[0066] Step 2: Preparing the Multi-Mineral Element Solution
[0067] 1) Pulverize celestite, medical ...
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