Multipurpose functional water and preparation method thereof
A functional water and multi-purpose technology, which can be applied in cosmetic preparations, chemical instruments and methods, pharmaceutical formulas, etc., can solve the problems of low extraction rate of active ingredients, unfavorable industrial application, and waste of raw materials, so as to shorten extraction time and enhance Skin elasticity, the effect of increasing the extraction rate
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preparation Embodiment 1
[0027] Step 1: Prepare leech extract
[0028] Soak 1000g leeches in distilled water for 12 hours, mince the soaked leeches with a meat grinder to obtain a leech homogenate; 2) add 15 times the volume of 4% Nacl solution to the leech homogenate to obtain a leech solution; 3 ) Treat the leech solution with 60kHz ultrasonic waves for 10 minutes to fully separate the active components of the leech; 4) After 5 hours, filter to obtain the filtrate and add ammonium sulfate to the filtrate, salt out for 8 hours, control the salting out temperature at 0-2°C, and collect the precipitate 5) Add distilled water to the collected precipitate to dissolve and transfer to a dialysis bag, and dialyze at 0°C for 48 hours to obtain 10 L of leech extract.
[0029] The main active ingredient in the leech extract is hirudin, and measuring the protein content of the extract can indicate the amount of hirudin. The determined protein content was 1.2 mg / ml.
[0030] Step 2: Prepare multi-mineral eleme...
preparation Embodiment 2
[0052] Step 1: Prepare leech extract
[0053] Soak 1000g leeches in distilled water for 24 hours, mince the soaked leeches with a meat grinder to obtain a leech homogenate; 2) add 20 times the volume of 4% Nacl solution to the leech homogenate to obtain a leech solution; 3 ) Treat the leech solution with 200kHz ultrasonic waves for 30 minutes to fully separate the active ingredients of the leech; 4) After 10 hours, filter to obtain the filtrate and add ammonium sulfate to the filtrate, salt out for 10 hours, and control the salting out temperature at 0-2°C, collect the precipitate 5) Add distilled water to the collected precipitate to dissolve and transfer to a dialysis bag, and dialyze at 0°C for 48 hours to obtain 10 L of leech extract. The determined protein content was 1.5 mg / ml.
[0054] Step 2: Prepare multi-mineral element solution
[0055] 1) Crush lapis lazuli, medical stone, vermiculite, and wood fish stone, and separate them through winnowing screen vibrators and ...
preparation Embodiment 3
[0064] Step 1: Prepare leech extract
[0065] Soak 1000g leeches in distilled water for 20 hours, mince the soaked leeches with a meat grinder to obtain a leech homogenate; 2) add 18 times the volume of 4% Nacl solution to the leech homogenate to obtain a leech solution; 3 ) Treat the leech solution with 100kHz ultrasonic waves for 20 minutes to fully separate the active components of the leech; 4) After 9 hours, filter to obtain the filtrate and add ammonium sulfate to the filtrate, salt out for 9 hours, control the salting out temperature at 0-2°C, and collect the precipitate 5) Add distilled water to the collected precipitate to dissolve and transfer to a dialysis bag, and dialyze at 0°C for 48 hours to obtain 10 L of leech extract. The determined protein content was 1.8 mg / ml.
[0066] Step 2: Prepare multi-mineral element solution
[0067] 1) Crush lapis lazuli, medical stone, vermiculite, and wood fish stone, and separate them through winnowing screen vibrators and sus...
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