Multipurpose functional water and preparation method thereof

A functional water and multi-purpose technology, which can be applied in cosmetic preparations, chemical instruments and methods, pharmaceutical formulas, etc., can solve the problems of low extraction rate of active ingredients, unfavorable industrial application, and waste of raw materials, so as to shorten extraction time and enhance Skin elasticity, the effect of increasing the extraction rate

Inactive Publication Date: 2013-05-08
陈丽娟 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the leech extraction method used in production is to crush the leeches and extract them by salting out separation method, but the problem of using this method is that the extraction rate of active ingredients such as hirudin contained in leeches is low, and the waste of raw materials is serious, which leads to the

Method used

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  • Multipurpose functional water and preparation method thereof
  • Multipurpose functional water and preparation method thereof
  • Multipurpose functional water and preparation method thereof

Examples

Experimental program
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Example

[0026] Preparation Example 1

[0027] Step 1: Prepare the Leech Extract

[0028] 1000g of leeches were soaked in distilled water for 12 hours, and the soaked leeches were minced with a meat grinder to obtain a leech homogenate; 2) 15 times the volume of 4% NaCl solution was added to the leech homogenate to obtain a leech solution; 3 ) The leech solution was ultrasonically treated at 60 kHz for 10 minutes to fully separate the active components of the leech; 4) After 5 hours, the filtrate was obtained by filtration, and ammonium sulfate was added to the filtrate, and the filtrate was salted out for 8 hours. The salting-out temperature was controlled at 0-2 °C, and the precipitate was collected. 5) Add distilled water to the collected precipitate to dissolve and transfer to a dialysis bag, and dialyze at 0°C for 48h to obtain 10L of leech extract.

[0029] The main active ingredient in the leech extract is hirudin, and measuring the protein content of the extract can indicate t...

Example

[0051] Preparation Example 2

[0052] Step 1: Prepare the Leech Extract

[0053] 1000g of leeches were soaked in distilled water for 24 hours, and the soaked leeches were minced with a meat grinder to obtain a leech homogenate; 2) 20 times the volume of 4% NaCl solution was added to the leech homogenate to obtain a leech solution; 3 ) The leech solution was ultrasonically treated at 200 kHz for 30 minutes to fully separate the active components of the leech; 4) After 10 hours, the filtrate was obtained by filtration, and ammonium sulfate was added to the filtrate, salting out for 10 hours, and the salting-out temperature was controlled at 0-2 °C, and the precipitate was collected. 5) Add distilled water to the collected precipitate to dissolve and transfer to a dialysis bag, and dialyze at 0°C for 48h to obtain 10L of leech extract. The protein content determined was 1.5 mg / ml.

[0054] Step 2: Preparing the Multi-Mineral Element Solution

[0055] 1) Pulverize celestite, me...

Example

[0063] Preparation Example 3

[0064] Step 1: Prepare the Leech Extract

[0065] 1000g of leeches were soaked in distilled water for 20 hours, and the soaked leeches were minced with a meat grinder to obtain a leech homogenate; 2) 18 times the volume of 4% NaCl solution was added to the leech homogenate to obtain a leech solution; 3 ) The leech solution was ultrasonically treated at 100 kHz for 20 minutes to fully separate the active components of the leech; 4) After 9 hours, the filtrate was obtained by filtration, and ammonium sulfate was added to the filtrate, salting out for 9 hours, the salting-out temperature was controlled at 0-2 °C, and the precipitate was collected. 5) Add distilled water to the collected precipitate to dissolve and transfer to a dialysis bag, and dialyze at 0°C for 48h to obtain 10L of leech extract. The protein content determined was 1.8 mg / ml.

[0066] Step 2: Preparing the Multi-Mineral Element Solution

[0067] 1) Pulverize celestite, medical ...

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Abstract

The invention relates to a preparation method of multipurpose functional water. The preparation method comprises the following steps of: preparing a leech extracting solution; preparing a polymineral element solution; and mixing the leech extracting solution and the polymineral element solution according to the mass ratio of 5:(200-500) at 10-20 DEG C so as to obtain the multipurpose functional water. The preparation method utilizes ultrasonic-assisted extraction, so that the extraction efficiency of effective constituents of leech can be enhanced, and the waste of the raw material is reduced. According to the prepared multipurpose functional water, Fe, Cu, I and Mn can play a role of protecting the effective constituents in the leech extracting solution, so that the target concentration of medicinal components of the leech extracting solution can increase, and furthermore, the use effect of the solution is improved.

Description

technical field [0001] The invention relates to multipurpose functional water and a preparation method thereof, more specifically, the invention relates to functional water containing leech extract and multi-mineral element solution and a preparation method thereof. Background technique [0002] Leeches have been used to treat diseases for more than 2,000 years. Modern scientific research shows that leeches can improve blood circulation, promote metabolism, reduce blood viscosity, maintain blood vessel elasticity, and smooth blood flow. It can be seen that the application range of leeches is wider, and the curative effect is definite in the treatment of a variety of chronic non-infectious diseases caused by blood stasis and sub-healthy people. disease, traumatic pain and breathing, nervous system and cancer, such as stroke, coronary heart disease, hyperlipidemia, hypertension, diabetes, benign prostatic hyperplasia, menopausal syndrome, dysmenorrhea, impotence, Alzheimer's d...

Claims

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Application Information

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IPC IPC(8): A61K35/62A61K8/98A61P29/00A61P27/02A61P9/10A61P9/12A61P3/06A61P9/00A61P17/10A61P17/08A61Q19/02A61Q19/00A61Q19/08C02F9/12
Inventor 陈丽娟钱仁刊向冬菊
Owner 陈丽娟
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