34results about How to "Mild degradation conditions" patented technology

The invention relates to a strain for generating keratinase and an application thereof. The strain is Stenotrophomonas maltophilia) DHHJ CGMCC No.2231 applied to the degradation of soft keratin, such as chicken feathers, pigeon feathers, cow hair, wool, hair and hard keratin, such as ox horns, goat horns, sheep hoofs and nail waste. Compared with other strains for degrading the keratin, the strain has mild fermentation conditions, is convenient to apply and can generate the keratinase with higher enzymatic activity and stability.

The invention provides a method for extracting and purifying anthocyanin from bilberry. The method comprises the following steps: (1) microbial degradation; (2) obtainment of a crude extract of bilberry anthocyanin by utilizing a water extraction process; (3) addition of a certain amount of a flocculant for impurity removal; (4) impurity removal and purification with macroporous resin; (5) concentration; and (6) spray drying. According to the invention, by combining aqueous-solution extraction and microbial degradation, an acylated stable structure of anthocyanin is realized through microbial degradation; in addition, the juice yield of bilberry is greatly improved through cellulase hydrolysis; thus, double purposes are achieved through one way, and interdisciplinary penetration and effective combination of biological science and chemical production are realized. The method provided by the invention also utilizes ZTC1 + 1 for flocculation and impurity removal, and solves the problems of easy loss of active ingredients and long production cycle in traditional water extraction and alcohol precipitation. The extraction method provided by the invention has the advantages of simple process operation, low production cost, no pollution, high extraction rate, and no damage to anthocyanin due to high temperature; and the anthocyanin prepared by using the method provided by the invention can be extensively applied to fields like food, health-care products and cosmetics, and has extremely high application values.

The invention discloses a method for preparing a monophenol compound through catalyzing degradation of lignin by using an ammino-complex. The method comprises the steps: adding the lignin, a metal salt, ammonia water, a peroxidant, an alkali and deionized water into a high-pressure reaction vessel, and carrying out stirring to form a uniform solution; carrying out a reaction for 100 to 240 minutes at the temperature of 120 DEG C to 180 DEG C, and carrying out cooling; and adjusting the pH value of a reaction solution to 2 to 3 with an acid, then, carrying out a reaction for 10 to 20 minutes at the temperature of 100 DEG C to 120 DEG C, carrying out cooling, then, carrying out filtration to collect filtrate, carrying out extraction on the filtrate with dichloromethane, separating an organic phase, and carrying out drying with anhydrous calcium chloride, thereby obtaining a monophenol compound mixed solution. According to the method, a stable ammino-complex solution is formed by using the metal salt and the ammonia water under alkaline conditions, so that the number of sites of contact between a catalyst and the lignin can be effectively increased compared with that in the conventional lignin oxidative degradation method; the degradation of the lignin can be efficiently catalyzed in an aqueous solution under moderate reaction conditions; and the method has the characteristics of high efficiency and low cost and is applicable to large-scale industrial production.

The invention discloses an electrodegradation recovery method of a carbon fiber reinforced composite material. According to the invention, electrocatalytic oxidation is adopted to oxidize thermosetting resin into a low-molecular-weight thermoplastic polymer and quickly dissolve the low-molecular-weight thermoplastic polymer in a mixed solvent, thereby separating and recovering carbon fibers and implementing resource reutilization. The conductivity of the mixed solvent and the solubility of the high-molecular polymer are good, so the catalytic oxidation efficiency of the fiber reinforced material can be obviously improved, a fiber recovery rate can reach 95% or above, and the thermosetting cross-linked epoxy resin is electrocatalytically oxidized and degraded into the low-molecular-weight thermoplastic polymer which is then dissolved in the mixed solvent, so separation and recycling are realized; and after electrocatalytic oxidation, water and the organic solvent can be directly distilled and recovered from electrodegradation liquid, and the electrodegradation liquid recovered through reduced-pressure distillation and washing liquid are mixed to serve as the electrodegradation liquid to be reused. The electrodegradation recovery method has the advantages of mild degradation conditions, simple process operation, no corrosion, a small amount of three wastes, environmental optimization and the like.

The invention discloses a preparation method of a bamboo liquid-collagen polypeptide composite adhesive. The preparation method comprises the following steps of: step (1). putting bamboo powder into alkali liquor in a reaction kettle, and performing primary alkali thermal degradation reaction to obtain bamboo liquid; step (2). adding leather meal to the bamboo liquid in the reaction kettle, and performing secondary alkali thermal degradation reaction on the leather meal to obtain bamboo liquid-collagen mixed degradation liquid; step (3). performing mechanical filter pressing on the bamboo liquid-collagen mixed degradation liquid to collect filtrate; and step (4). adjusting the pH of the filtrate, adding a crosslinking agent and a plasticizer, stirring and reacting to obtain the bamboo liquid-collagen polypeptide composite adhesive. The preparation method of the bamboo liquid-collagen polypeptide composite adhesive utilizes biomass resources and has low production cost.

The invention discloses a screening method for preparing medium-molecular-weight dextran strains, belonging to the field of dextran. The present invention utilizes the microorganisms on the grape skins for cultivation, and modifies the cultured microorganisms with α-aminoisobutyric acid under the irradiation of an X-ray apparatus, filters the modified bacterial cell suspension, and smears the bacterial cell suspension Cultivate on a medium prepared by biotin, add 6-benzylaminopurine ethanol solution during the cultivation process, inoculate the cultured mycelium into a screening medium for cultivation, sieve, and finally collect strains. The present invention Using the microorganisms on the grape skin for modification, cultivation, and screening, the process is simple and the cost is low. The strains obtained by screening not only have high activity and good stability, but also use them to degrade dextran, and can obtain medium molecular weight dextran with uniform molecular weight distribution. , the quality and quality of dextran are good, the conditions of the degradation process are mild, and the energy consumption is small.

The invention relates to a degradable thermosetting 3D printing mold and a preparation method thereof. The preparation method of the degradable thermosetting 3D printing mold comprises the following steps: (1) taking vanillin, pentaerythritol and methacryloyl chloride as raw materials, and synthesizing a difunctional acrylate monomer containing a degradable diacetal structure through a two-step method; and (2) mixing the difunctional acrylate monomer containing the diacetal structure, 4-acryloylmorpholine and a photoinitiator, and carrying out photocuring 3D printing to obtain the degradable thermosetting 3D printing mold. The prepared degradable thermosetting 3D printing mold is high in printing precision, the preparation method is environmentally friendly, the preparation process is simple, industrial production is easy, the obtained resin has excellent comprehensive performance, and the method for preparing the high-performance degradable thermosetting 3D printing mold through the thermosetting resin is created.

The invention discloses an ionic liquid-complex microbial inoculum system which comprises ionic liquid and a complex microbial inoculum. The ionic liquid and the complex microbial inoculum form a coupling system for enzymolysis of lignocellulose; the ionic liquid contains 1-ethyl-3-methylimidazole ferric chloride salt; and the complex microbial inoculum comprises fermentation liquor of wet cellulomonas, streptomyces albus, candida tropicalis and white rot fungi. In an enzymolysis test, the highest cellulase activity of enzymatic hydrolysate is 185.1 U.mL<1>, the degradation weight loss rate ofgarden biomass reaches 52.6% and is respectively increased by 59.0% and 66.5% with respect to that of a only complex microbial inoculum, and lignin content of enzymolysis residues is reduced by 35.2%. In the enzymolysis application, the enzymolysis period of the garden biomass is 18 to 26 days, the high-temperature period lasts for 12 to 21 days, the highest temperature reaches 62 to 72 DEG C, the final degradation weight loss rate is 47.2 to 54.2%, and the lignin content is reduced to 8.5 to 10.6%. The ionic liquid-complex microbial inoculum system is simple and convenient in preparation process and outstanding in enzymolysis efficiency, and can meet the social requirements of intensive and resource treatment and utilization of agriculture and forestry biomass and the development conceptof green circulation.

The present invention relates to an earthworm fermentation liquid-based feed additive, and a preparation method and an application thereof. The feed additive comprises earthworm fermentation liquid asa basic component, and an amino sugar oligomer and a sodium metabisulfite compound as a synergistic component; the amino sugar oligomer is a product of chitosan degraded by aspergillus tamarii; the sodium metabisulfite compound is composed of core particles containing sodium metabisulfite and an enteric coating coating the core particles; and a mass ratio of the amino sugar oligomer to the sodiummetabisulfite compound is (4.0-4.5):1. The feed additive has significant benefits for growth and fattening, survival rate and immune system of livestock and poultry animals, significantly reduces occurrence of toxic effects such as decreased feed intake, growth rate, immunity and survival rate of livestock and poultry caused by vomiting toxins, and can more efficiently improve animal production efficiency.

The invention discloses a method for double-frequency composite ultrasonic degradation of phenol in rural sewage, and belongs to the technical field of phenol degradation. According to the method, double-frequency composite ultrasonic treatment is adopted to perform ultrasonic treatment on a to-be-degraded phenol solution for 10-30 min; the double-frequency composite ultrasonic degradation rate onphenol can reach 83.74%, and is improved by 45.23% and 51.11% compared with the degradation rate of single probe type ultrasonic treatment and single groove type ultrasonic treatment. Therefore, it is obviously shown that the degradation effect of dual-frequency composite ultrasonic synergistic degradation on phenol in water is ideal.