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Strain for generating keratinase and application thereof

A keratinase and keratin technology, which is applied to the field of new strains producing keratinase, can solve the problems of less research on keratinase and limited research objects, and achieves the effects of high keratinase activity, mild degradation conditions and good stability

Inactive Publication Date: 2009-11-18
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, the research object is also extremely limited, mainly focusing on the research of Streptomyces flexneri, and less research on the production of keratinase by bacterial fermentation

Method used

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  • Strain for generating keratinase and application thereof
  • Strain for generating keratinase and application thereof
  • Strain for generating keratinase and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Buried a large number of feathers collected from the market in the moist soil outdoors for enrichment and cultivation until the feathers were significantly degraded, and sufficient water was maintained during the period. Take 1 g of the above-mentioned rotten chicken feathers in enrichment medium 1 (0.5 g of beef extract, 1 g of peptone, 0.5 g of sodium chloride, pH7.4-7.6, 100 mL), and incubate at 28 °C and 120 rpm for 48 h. Then take 2 mL of its culture solution and transfer it to enrichment medium 2 (2 g of soluble starch, 2 g of feather powder a, 0.5 g of sodium chloride, pH7.4-7.6, 100 mL), and further enrich and cultivate for 48 h at 28 ° C and 120 r / min . Take 100 μL of the final enrichment solution and spread it on the plate of phosphoric acid digested protein medium (soluble starch 2g, phosphoric acid digested protein 2.5g, agar powder 2g, sodium chloride 0.5g, pH7.4-7.6, 100mL) plate, and screen out the growing single Bacterial colonies, with beef extract...

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Abstract

The invention relates to a strain for generating keratinase and an application thereof. The strain is Stenotrophomonas maltophilia) DHHJ CGMCC No.2231 applied to the degradation of soft keratin, such as chicken feathers, pigeon feathers, cow hair, wool, hair and hard keratin, such as ox horns, goat horns, sheep hoofs and nail waste. Compared with other strains for degrading the keratin, the strain has mild fermentation conditions, is convenient to apply and can generate the keratinase with higher enzymatic activity and stability.

Description

technical field [0001] The invention belongs to the field of microbial fermentation strains, in particular to a novel strain producing keratinase and its application. Background technique [0002] Keratin exists in nature in large quantities as structural proteins such as skin, hair, feathers, quills, hooves, horns, and claws of vertebrates. It is a hard protein with strong resistance. Its structure is through intermolecular disulfide bonds, hydrogen It has high stability after the action of bonds, salt bonds and other bonds. It is insoluble in water, salt, dilute acid and dilute alkali under normal conditions, and cannot be digested by general proteases (such as pepsin, trypsin and papain). hydrolysis. Keratinase is a class of enzymes that can hydrolyze keratin, and some keratin can also hydrolyze various proteins except keratin. [0003] Keratinase can degrade keratin, so it has broad application prospects in feed industry, food industry, leather processing, detergent in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/52
Inventor 张启曹张军周美华王晶陈丽魏冬凯
Owner DONGHUA UNIV
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