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A kind of method utilizing bacillus pumilus to degrade aflatoxin b1 or m1

A technology of Bacillus pumilus and aflatoxin, which is applied in the field of microorganisms, can solve the problems of by-products and chemical reagent residues, damage to product nutrients, and lower product quality, and achieve safe and non-toxic sources, easy operation, and mild degradation conditions. Effect

Active Publication Date: 2017-01-18
BEIJING YUANSHENGYUAN SCI & TECH DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although physical and chemical methods have a certain effect on degrading aflatoxin, they will inevitably destroy the nutritional content of the product, reduce the quality of the product, and may also produce by-products and chemical reagent residues, which will cause certain harm

Method used

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  • A kind of method utilizing bacillus pumilus to degrade aflatoxin b1 or m1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Preparation medium

[0025] Add 3g of beef extract, 5g of peptone, and 10g of NaCl into 1L of distilled water, stir to dissolve, adjust the pH value to about 7.2, and then steam sterilize at 121°C for 20min to prepare the seed medium;

[0026] 5g lactose, 10g yeast extract, 1g MgCl 2 Add it into 1L of distilled water, stir to dissolve, adjust the pH value to about 7.2, and then steam sterilize at 121° C. for 20 minutes to prepare a fermentation medium.

[0027] 2. Fermentation

[0028] Inoculate the Bacillus pumilus after activation on the plate into the above-prepared seed medium, and culture it for 12 hours at a temperature of 37°C and an oscillation speed (rotational speed) of 140 r / min to obtain a seed solution;

[0029] According to the inoculum amount of 5%, the above-prepared fermentation medium was inserted, and cultured for 72 hours at a temperature of 37° C. and a shaking speed of 140 r / min to obtain a fermentation broth.

Embodiment 2

[0031] 8g lactose, 8g yeast extract, 0.5g MgCl 2 Add it into 1L of distilled water, stir to dissolve, adjust the pH value to about 7.2, and then steam sterilize at 121° C. for 20 minutes to prepare a fermentation medium.

[0032] The seed solution prepared in Example 1 was inserted into the fermentation medium prepared above according to the inoculum amount of 3%, and cultured for 48 hours at a temperature of 35° C. and a shaking speed of 160 r / min to obtain a fermentation broth.

Embodiment 3

[0034] 10g lactose, 5g yeast extract, 1.5g MgCl 2 Add it into 1L of distilled water, stir to dissolve, adjust the pH value to about 7.2, and then steam sterilize at 121° C. for 20 minutes to prepare a fermentation medium.

[0035] The seed solution prepared in Example 1 was inserted into the above-prepared fermentation medium according to the inoculum amount of 1%, and cultured for 96 hours at a temperature of 39° C. and a shaking speed of 120 r / min to obtain a fermentation broth.

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Abstract

The invention provides a Bacillus pumilus and application thereof in degrading aflatoxin. The collection number of the Bacillus pumilus is CGMCC No.9086, and the metabolite can effectively degrade the aflatoxin.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus pumilus and its application in degrading aflatoxin. Background technique [0002] Aflatoxins are a class of compounds with similar chemical structures, mainly produced by Aspergillus flavus and A. parasiticus, B1, B2, G1, G2, M1, M2 have been isolated and identified There are more than a dozen kinds of aflatoxins, the basic structures of which are dihydrofuran ring and coumarin. Aflatoxin is a highly toxic and highly toxic substance. Its harmfulness mainly lies in its destructive effect on the liver tissue of humans and animals. In severe cases, it can cause liver cancer or even death. [0003] Aspergillus flavus and Aspergillus parasiticus exist widely in the air and soil, and after they infect peanuts, corn and other food crops, they will produce aflatoxins in these crops and their downstream products. Aflatoxin B1 is the most common in naturally...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L5/20C12R1/07
Inventor 于赞祝传斌牛旼
Owner BEIJING YUANSHENGYUAN SCI & TECH DEV
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