A kind of mushroom oligosaccharide with excellent antioxidant activity and preparation method thereof

Abstract

The invention discloses a lentinan oligosaccharide with excellent antioxidant activity and a preparation method thereof. The method steps are: (1) mixing lentinan with distilled water, adding β-mannanase, adjusting the pH=5-7, ‑60°C in a constant temperature water bath, stirred and boiled for 13‑17 minutes to stop the reaction, filtered, concentrated the filtrate, dialyzed the concentrated solution to remove undegraded macromolecular lentinan, concentrated the dialysate, and freeze-dried to obtain crude lentinan oligosaccharides; (2 ) According to the proportion, take the crude lentinus oligosaccharides and dissolve them in distilled water, add an aqueous ethanol solution, ethanol precipitation, centrifuge, and concentrate the supernatant to lyophilize; (3) separate and purify the lyophilized product through a Sephadex G‑15 column. The invention adopts an enzymatic hydrolysis method to degrade the lentinan, and the degradation condition is mild. The preparation process is simple and the yield is high. The prepared oligosaccharide has excellent antioxidant activity and can be used in health products, food and medicine and antioxidant additives.

Description

technical field [0001] The invention belongs to the technical field of functional food or health products, and in particular relates to a lentinus oligosaccharide with excellent antioxidant activity and a preparation method. Background technique [0002] Shiitake mushroom, the second largest edible fungus in the world, is delicious and nutritious. It is a kind of food with the same source of medicine and food, and has high nutritional, medicinal and health value. Lentinan is an active ingredient with various biological activities in Lentinus edodes. Since Chihara et al. first used hot water extraction to extract 6 kinds of polysaccharides from the fruiting body of Lentinus edodes, Lentinan has received extensive attention and research. Studies have reported that lentinan has biological activities such as anti-tumor, immune regulation, anti-virus, anti-infection, anti-inflammation, anti-oxidation, hypoglycemic and hypolipidemic. [0003] Although the type of glycosidic bond ...

AI Technical Summary

Problems solved by technology

In addition, the current research on shiitake mushrooms is mostly focused on lentinan. The research on the preparation of shiitake oligosaccharides is mainly through chemical synthesis. However, due to the complexity of monosaccharide-based molecules, multi-step protection and deprotection operations are involved in the synthesis process. 1. The formation of glycosidic bonds has problems such as certain regioselectivity and stereoselectivity. Therefore, the chemical synthesis method is only suitable for a small amount of g-grade products in the laboratory, and it is difficult to prepare in large quantities.

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