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Lentinan oligosaccharide having excellent anti-oxidation activity, and preparation method thereof

A technology of antioxidant activity and lentinan, which is applied to the functions of food ingredients, polysaccharide/gum-containing food ingredients, food extraction, etc., can solve the problems of difficult mass production, etc., and achieve simple preparation process, high yield, and excellent antioxidant activity Effect

Active Publication Date: 2018-07-10
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the current research on shiitake mushrooms is mostly focused on lentinan. The research on the preparation of shiitake oligosaccharides is mainly through chemical synthesis. However, due to the complexity of monosaccharide-based molecules, multi-step protection and deprotection operations are involved in the synthesis process. 1. The formation of glycosidic bonds has problems such as certain regioselectivity and stereoselectivity. Therefore, the chemical synthesis method is only suitable for a small amount of g-grade products in the laboratory, and it is difficult to prepare in large quantities.

Method used

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  • Lentinan oligosaccharide having excellent anti-oxidation activity, and preparation method thereof
  • Lentinan oligosaccharide having excellent anti-oxidation activity, and preparation method thereof
  • Lentinan oligosaccharide having excellent anti-oxidation activity, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of Lentinan:

[0027] (1) Get shiitake mushrooms and dry and pulverize them, and cross a 60-mesh sieve;

[0028] (2) According to the mass ratio of 1:30, mix the shiitake mushroom powder obtained in step (1) with distilled water, boil and extract at 100°C for 30 minutes, filter, repeat the extraction of the filter residue once, and concentrate the filtrate to 20% of the volume of distilled water to obtain Concentrated solution; in proportion, add 0.9 g of papain with an enzyme activity of 2800 U / g to 500 mL of concentrated solution to enzymatically hydrolyze glycoprotein to obtain papain enzymatic hydrolyzate; according to the volume ratio of sevag reagent and papain enzymatic hydrolyzate is 1: Add sevag reagent to the papain enzymatic hydrolysis solution at a ratio of 5, mix fully in the separatory funnel, let stand to separate layers, release the organic layer containing protein precipitation in the lower layer, leave the upper layer solution and repeat th...

Embodiment 2

[0030] A preparation method of lentinus oligosaccharides with excellent antioxidant activity, comprising the steps of:

[0031] (1) Mix the lentinan prepared in Example 1 with distilled water at a mass ratio of 1:80, add β-mannanase to make the final concentration of β-mannanase 75U / ml, and adjust the pH= 6.0, in a constant temperature water bath at 50°C, stir for 72 hours, boil for 15 minutes to stop the reaction, filter, concentrate the filtrate to 15% of the volume of the filtrate, and dialyze the concentrated solution through a dialysis bag with a molecular weight cut-off of 6KDa to remove undegraded macromolecular mushrooms Polysaccharides, the dialysate was concentrated and freeze-dried to obtain crude mushroom oligosaccharides;

[0032] (2) Proportionally, take 5g of crude lentinus oligosaccharides and dissolve them in 80mL of distilled water, add an aqueous ethanol solution with a volume concentration of 95% to make the volume concentration of ethanol in the mixed solu...

Embodiment 3

[0035] A preparation method of lentinus oligosaccharides with excellent antioxidant activity, comprising the steps of:

[0036] (1) Mix the lentinan prepared in Example 1 with distilled water at a mass ratio of 1:50, add β-mannanase to make the final concentration of β-mannanase 30 U / ml, and adjust the pH= 5. In a constant temperature water bath at 40°C, stir for 96 hours, boil for 13 minutes to stop the reaction, filter, concentrate the filtrate to 10% of the volume of the filtrate, and dialyze the concentrate through a dialysis bag with a molecular weight cut-off of 8KDa to remove undegraded macromolecular mushrooms Polysaccharides, the dialysate was concentrated and freeze-dried to obtain the crude mushroom oligosaccharides;

[0037] (2) Proportionally, take 5g of crude lentinus oligosaccharides and dissolve them in 50mL of distilled water, add an aqueous ethanol solution with a volume concentration of 95% to make the volume concentration of ethanol in the mixed solution 70...

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Abstract

The invention discloses a lentinan oligosaccharide having an excellent anti-oxidation activity, and a preparation method thereof. The method comprises the following steps: (1) mixing lentinan with distilled water, adding beta-mannanase, adjusting the pH value of the obtained solution to 5-7, performing stirring reacting boiling in 40-60 DEG C constant-temperature water bath for 13-17 min, stoppingthe reaction, filtering the obtained reaction solution, concentrating the obtained filtrate, dialyzing the obtained concentrate to remove undegraded macromolecular lentinan, and concentrating and lyophilizing the obtained dialysate to obtain crude lentinan oligosaccharides; (2) taking the crude lentinan oligosaccharides in proportion, dissolving the crude lentinan oligosaccharides in distilled water, adding an ethanol-water solution to perform alcohol precipitation, centrifuging the precipitated solution, and concentrating and lyophilizing the obtained supernatant; and (3) separating and purifying the obtained lyophilized product through a Sephadex G-15 column. The lentinan oligosaccharides are degraded through adopting an enzymatic hydrolysis technology, and degradation conditions are mild. The preparation process is simple, and the yield is high. The prepared oligosaccharide has excellent anti-oxidation activity, and can be used in healthcare products, foods and drugs, and anti-oxidation additives thereof.

Description

technical field [0001] The invention belongs to the technical field of functional food or health products, and in particular relates to a lentinus oligosaccharide with excellent antioxidant activity and a preparation method. Background technique [0002] Shiitake mushroom, the second largest edible fungus in the world, is delicious and nutritious. It is a kind of food with the same source of medicine and food, and has high nutritional, medicinal and health value. Lentinan is an active ingredient with various biological activities in Lentinus edodes. Since Chihara et al. first used hot water extraction to extract 6 kinds of polysaccharides from the fruiting body of Lentinus edodes, Lentinan has received extensive attention and research. Studies have reported that lentinan has biological activities such as anti-tumor, immune regulation, anti-virus, anti-infection, anti-inflammation, anti-oxidation, hypoglycemic and hypolipidemic. [0003] Although the type of glycosidic bond ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/12C12P19/04C12P19/00A23L33/125
CPCA23V2002/00A23L33/125C12P19/00C12P19/04C12P19/12C12P19/14A23V2200/30A23V2250/208A23V2250/51A23V2300/14
Inventor 乔建军朱宏吉宋倩倩杨慧薛艳婷张雷田丽财音青格乐
Owner TIANJIN UNIV
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