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Systems and methods for controlling cell behavior

A cell and gene technology, applied in botany equipment and methods, biochemical equipment and methods, animal cells, etc., can solve problems such as limited time window, low recovery efficiency of manipulated cells, deterioration of toxic effects of genetic manipulation, etc.

Pending Publication Date: 2021-09-07
HANGZHOU QIHAN BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, gene editing technology still has some drawbacks
For example, genetic manipulation of cells (e.g., isolated cells) can be associated with toxicity and premature cell death, and can lead to inefficient recovery of manipulated cells
In some cases, multiple sequential genetic manipulations may be required and can accelerate or even worsen the toxic effects of genetic manipulation
Gene editing of primary, terminally differentiated cells with limited proliferative capacity can create additional technical challenges due to the limited time window for genetic manipulation and the limitation of recovering enough cells for suitable downstream applications

Method used

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  • Systems and methods for controlling cell behavior

Examples

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Embodiment 1

[0426] Example 1. Construction of Exemplary Polynucleotide Sequences for Modulating Cell Immortalization.

[0427] Targeted constructs for modulating cellular immortalization can be designed and cloned into suitable vectors with multiple cloning sites. The targeting construct may be a polynucleic acid comprising the coding sequence of the TERT gene flanked by loxP sites ( figure 1 A. figure 1 B), and can be constructed as follows. The individual coding and non-coding sequences may be inserted or joined in any order, as determined by one of skill in the art, and may depend, for example, on the choice and availability of restriction sites. The coding sequence of the TERT gene can be appropriately inserted into the clone, operably linked to a promoter suitable for the cell. In the given example, the promoter mEF1a is linked upstream of TERT. A WPRE polyA termination sequence was linked downstream of the coding sequence and upstream of the 3' Lox site. Additionally, the r...

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Abstract

A highly specific and beneficial T cell therapy can be provided by controlled ex vivo induction or amplification to produce antigen-specific immune cells (like T cells). The invention provides T cell manufacturing methods and therapeutic T cell compositions, for example, for treating subjects with cancer and other conditions, diseases and disorders, as well as individual antigen-specific T cell therapies.

Description

[0001] cross reference [0002] This application claims the international patent application PCT / CN2020 / 077533 filed on March 3, 2020, the international patent application PCT / CN2020 / 077545 filed on March 3, 2020, and the international patent filed on March 3, 2020 Application PCT / CN2020 / 077535, international patent application PCT / CN2020 / 077547 filed on March 3, 2020, international patent application PCT / CN2020 / 077536 filed on March 3, 2020 and filed on March 3, 2020 Priority to filed International Patent Application PCT / CN2020 / 077557, each of which is incorporated herein by reference in its entirety. Background technique [0003] Gene editing technology has developed rapidly over the past few decades. Gene editing can be used for a variety of applications, including scientific research, medical diagnostics, and therapeutic development. Gene editing may involve one or more manipulations of genes, eg, adding, deleting, or replacing target genes or portions thereof at single...

Claims

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Application Information

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IPC IPC(8): C12N15/85C12N5/10C12N15/54A01K67/027
CPCC12N15/8509C12N5/0602C12N9/1276C12Y207/07049A01K67/0278C12N2510/04C12N2800/30A01K2227/108A01K2267/02
Inventor 高杨滨冯旭许伟宏
Owner HANGZHOU QIHAN BIOTECHNOLOGY CO LTD
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