Concentration gradient generation device for gel 3D cell culture and preparation and application method

Abstract

The invention discloses a concentration gradient generation device for gel 3D cell culture. The device comprises multi-stage mixing and concentration gradient generation modules and gel chambers of multi-stage concentration gradient ranges, wherein a plurality of gel samples can be carried simultaneously; through application of sectional concentration gradients, detection and evaluation on cell behaviors in gel within different concentration gradient ranges can be performed simultaneously; and through setup of the multi-stage concentration gradient ranges, samples of different concentration spans can be simultaneously detected on a same fluid chip. The fluid chip provided by the invention has the advantages of being small in volume, low in cost, good in integration with a trace amount of gel, and the like, and has wide application prospects in aspects such as cell physiology, pathology, drug screening, biomedicines and environmental monitoring. Meanwhile, the invention further discloses a preparation method and an application method of the concentration gradient generation device.

Description

technical field [0001] The invention belongs to the technical field of microanalytical instruments, and in particular relates to a concentration gradient generating device for gel 3D cell culture, a preparation method and an application method. Background technique [0002] With the development of micro-electro-mechanical systems, analytical instruments, chemical instruments, and medical devices are also more inclined to miniaturization, automation, integration and portability. A concentration gradient generating device is usually a micro-device that generates a molecular concentration gradient on a microscale and loads it on cells or cell populations for cell biology research. In the human body, concentration gradients of biomolecules control many fundamental cellular functions, such as development, immune response, wound healing, embryogenesis, and cancer metastasis. Biological processes rely on biomolecular gradients to augment and modulate cellular signaling pathways. ...

AI Technical Summary

Problems solved by technology

While injecting the gel, it will cause the gel to solidify in advance and remain in the microchannel, or cause the molecular concentration gradient range of the gel sample to be relatively single; and there is a problem that only one gel sample can be tested at a time; at the same time, due to Molecular diffusion is completely spontaneous, making the generated gradient difficult to quantify and dynamically transform, and it is also difficult to form an external molecular loading form with a variety of molecular concentration gradient ranges on the same fluid chip

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