A concentration gradient generating device, preparation and application method for gel 3D cell culture

Abstract

The invention discloses a concentration gradient generation device for gel 3D cell culture, which includes a multi-stage mixing and concentration gradient generation module, and a gel chamber with a multi-stage concentration gradient range, which can simultaneously load multiple gel samples , and through the application of segmental concentration gradients, the detection and evaluation of cell behavior in gels in different concentration gradient ranges is carried out at the same time; the establishment of multi-level concentration gradient ranges can simultaneously detect cells in different concentrations on the same fluid chip. span samples; the fluid chip provided by the present invention has the advantages of small size, low cost, and good integration performance with microgels, and has broad applications in in vitro tissue reconstruction, cell physiology, pathology, and drug screening, biomedicine, and environmental monitoring. Application prospect; at the same time, the invention also discloses its preparation method and application method.

Description

technical field [0001] The invention belongs to the technical field of microanalytical instruments, and in particular relates to a concentration gradient generating device for gel 3D cell culture, a preparation method and an application method. Background technique [0002] With the development of micro-electro-mechanical systems, analytical instruments, chemical instruments, and medical devices are also more inclined to miniaturization, automation, integration and portability. A concentration gradient generating device is usually a micro-device that generates a molecular concentration gradient on a microscale and loads it on cells or cell populations for cell biology research. In the human body, concentration gradients of biomolecules control many fundamental cellular functions, such as development, immune response, wound healing, embryogenesis, and cancer metastasis. Biological processes rely on biomolecular gradients to augment and modulate cellular signaling pathways. ...

AI Technical Summary

Problems solved by technology

While injecting the gel, it will cause the gel to solidify in advance and remain in the microchannel, or cause the molecular concentration gradient range of the gel sample to be relatively single; and there is a problem that only one gel sample can be tested at a time; at the same time, due to Molecular diffusion is completely spontaneous, making the generated gradient difficult to quantify and dynamically transform, and it is also difficult to form an external molecular loading form with a variety of molecular concentration gradient ranges on the same fluid chip

Images