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Three-dimensional graphene foam/natural polysaccharide-based hydrogel composite scaffold and its preparation method

A graphene foam, natural polysaccharide technology, applied in general culture methods, biochemical equipment and methods, cell culture supports/coatings, etc., can solve the problem of maintaining three-dimensional structural integrity, spatial discontinuity, insufficient flexibility, etc. problem, to achieve the effect of good conductivity, less defects and good quality

Active Publication Date: 2022-07-29
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high rigidity and porous structure of three-dimensional graphene foam, there is a problem of insufficient flexibility, which makes it difficult to maintain the integrity of the three-dimensional structure under the pressure of some specific tissues when it is applied in vivo
At present, the three-dimensional graphene scaffold formed by self-assembly of graphene oxide has the disadvantage of spatial discontinuity, and its electrical conductivity is relatively poor.

Method used

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  • Three-dimensional graphene foam/natural polysaccharide-based hydrogel composite scaffold and its preparation method
  • Three-dimensional graphene foam/natural polysaccharide-based hydrogel composite scaffold and its preparation method
  • Three-dimensional graphene foam/natural polysaccharide-based hydrogel composite scaffold and its preparation method

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preparation example Construction

[0023] Another aspect of the embodiment of the present invention also provides a preparation method of a three-dimensional graphene foam / natural polysaccharide-based hydrogel composite scaffold, comprising:

[0024] Provide three-dimensional graphene foam;

[0025] Double bond modification is performed on the natural polysaccharide to obtain a natural polysaccharide cross-linking agent;

[0026] And, the natural polysaccharide cross-linking agent and the photoinitiator are mixed and added to the three-dimensional graphene foam, and reacted under ultraviolet light to obtain the three-dimensional graphene foam / natural polysaccharide-based hydrogel composite scaffold.

[0027] Further, the natural polysaccharide includes any one of glucan, hyaluronic acid, chitosan, and laminarin, and is not limited thereto.

[0028] Further, the molecular weight of the laminarin is 5k-500k, and the modification degree is 10%-80%.

[0029] Further, the photoinitiator includes, but is not limite...

Embodiment 1

[0044] Example 1: Preparation of three-dimensional graphene foam / laminarin-based hydrogel composite scaffold

[0045] (1) three-dimensional graphene foam is prepared by chemical vapor deposition;

[0046](2) Weigh 400 mg of laminarin, add 4 mL of dimethyl sulfoxide, 66.8 mg of 4-lutidine, and 250 mg of glycidyl methacrylate in sequence under nitrogen protection, and stir until completely dissolved. The mixed solution was stirred at room temperature in the dark for 48 h; an equimolar amount of concentrated hydrochloric acid solution was added to neutralize the 4-lutidine in the solution to terminate the reaction. The mixed solution after the reaction was dialyzed with deionized water for 5 days (molecular weight cut-off was 3.5k), and the laminarin cross-linking agent of double bond modification was obtained by freeze-drying, and the reaction was as follows:

[0047]

[0048] Among them, the molecular weight of laminarin is 5k-500k.

[0049] (3) Mix the obtained laminarin ...

Embodiment 2

[0057] Example 2: Preparation of three-dimensional graphene foam / laminarin-based hydrogel composite scaffold

[0058] (1) three-dimensional graphene foam is prepared by chemical vapor deposition;

[0059] (2) Weigh 200 mg of laminarin, add 2 mL of dimethyl sulfoxide, 33.4 mg of 4-lutidine, and 125 mg of glycidyl methacrylate in sequence under nitrogen protection, and stir until completely dissolved. The mixed solution was stirred at room temperature in the dark for 30 h; an equimolar amount of concentrated hydrochloric acid solution was added to neutralize the 4-lutidine in the solution to terminate the reaction. The reacted mixed solution was dialyzed with deionized water for 4 days (molecular weight cut-off was 2k), and lyophilized to obtain a double bond-modified laminarin cross-linking agent, wherein the molecular weight of laminarin was 5k-500k.

[0060] (3) Mix the obtained laminarin cross-linking agent with 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone uniformly...

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Abstract

The invention discloses a three-dimensional graphene foam / natural polysaccharide-based hydrogel composite scaffold and a preparation method thereof. The composite scaffold includes: three-dimensional graphene foam, and a natural polysaccharide-based hydrogel combined with the three-dimensional graphene foam. The three-dimensional graphene foam / natural polysaccharide-based hydrogel composite scaffold of the present invention has both the advantages of graphene and hydrogel, and the natural polysaccharide-based hydrogel also significantly improves the toughness of the three-dimensional graphene foam, and makes the composite scaffold It has good electrical conductivity, and when it is applied to cell culture, it can make cells adhere and spread, and can regulate cell behavior through chemically bonded biological factors.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a three-dimensional graphene foam / natural polysaccharide-based hydrogel composite scaffold and a preparation method thereof, and the application of the composite scaffold in culturing cells. Background technique [0002] Graphene is a two-dimensional carbon nanomaterial composed of single or few layers of carbon atoms. It is the thinnest material so far and has excellent physical and chemical properties, such as extremely high electron mobility, tunable optical properties, high Mechanical strength and good thermal and electrical conductivity have received extensive attention in the fields of materials, physics and chemistry. The self-supporting 3D graphene foam prepared by the template method has less defect stacking, which can extend the excellent physical and chemical properties of 2D graphene at the nanoscale to the 3D macroscopic field. In the process of biologi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/28C08J3/075C08L5/00C08K7/24C12N5/00
CPCC08J3/28C08J3/075C12N5/0075C08J2305/00C08K7/24C12N2533/20C12N2533/70
Inventor 程国胜冯林郝莹马迅朱墨
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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