Single-layer parallel fiber pattern with cell adhesion contrast characteristic and preparation method thereof
A cell adhesion, parallel fiber technology, applied in biochemical equipment and methods, cell culture supports/coatings, general culture methods, etc., can solve the problems of lack of effective fiber material platform, influence, etc., and achieve excellent anti-cell adhesion properties , Simple process, good controllability
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Embodiment 1
[0055] A method for preparing a single-layer parallel fiber pattern with contrasting characteristics of cell adhesion, the specific steps are as follows:
[0056] (1) Modify the base:
[0057] The material of the substrate is a gold-sprayed glass sheet, and the modification of the substrate includes the steps of substrate cleaning, graft modification treatment and post-treatment in sequence;
[0058] (1.1) Substrate cleaning: soak the substrate in absolute ethanol for 20 minutes;
[0059] (1.2) Grafting modification treatment: put the cleaned base material into the grafting reagent solution in a closed reaction vessel, use ethanol as a solvent, and react for 2 days under a nitrogen protection atmosphere at 25°C, wherein the grafting reagent is (11-Mercaptoundecyl)hexa(ethylene glycol) (CAS No.: 130727-44-5), the concentration of the grafting reagent solution is 3mM;
[0060] (1.3) Post-treatment: ultrasonically clean the graft-modified substrate with absolute ethanol for 8 min...
Embodiment 2
[0070] A method for preparing a single-layer parallel fiber pattern with contrasting characteristics of cell adhesion, the specific steps are as follows:
[0071] (1) Modify the base:
[0072] The material of the substrate is a glass sheet, and the modification of the substrate sequentially includes the steps of substrate cleaning, plasma treatment, grafting modification treatment and post-treatment;
[0073] (1.1) Substrate cleaning: wipe the substrate with lens tissue, put it in piranha lotion for 30 minutes, and then ultrasonically clean it with ultrapure water;
[0074] (1.2) Plasma treatment: the gas source used is high-purity oxygen, the treatment vacuum is 30Pa, the treatment power is 200W, and the treatment time is 30min;
[0075] (1.3) Grafting modification treatment: put the substrate after plasma treatment into the grafting reagent solution in a closed reaction vessel, use toluene as the solvent and triethylamine as the catalyst, and react under a nitrogen atmosphe...
Embodiment 3
[0082] The preparation method of a single-layer parallel fiber pattern with contrasting cell adhesion characteristics is basically the same as that in Example 2, except that the moving speed of the screw slide table is set to 0.2 cm / min, and the finally prepared pattern with contrasting cell adhesion characteristics A single-layer parallel fiber pattern, including a substrate and a single layer of parallel-arranged fibers fixed on the substrate, the two ends of the single-layer parallel-arranged fibers are fixed on the substrate by polymethacrylic anhydride gelatin, the fiber diameter is 9 μm, adjacent The distance between two fibers is 50 μm (the prepared pattern is as figure 2 The small-diameter densely packed pattern shown in ).
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