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Single-layer parallel fiber pattern with cell adhesion contrast characteristic and preparation method thereof

A cell adhesion, parallel fiber technology, applied in biochemical equipment and methods, cell culture supports/coatings, general culture methods, etc., can solve the problems of lack of effective fiber material platform, influence, etc., and achieve excellent anti-cell adhesion properties , Simple process, good controllability

Pending Publication Date: 2021-12-10
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in the above-mentioned similar studies, there is the following obvious interference of "background adhesion": the adhesion interference of cells through the interfiber space and the background substrate material (such as attachment image 3 shown in the second and third columns in ), cell adhesion interference between adjacent fibers in the up-down or left-right direction (such as attached image 3 shown in the third column in
The interference of the "background adhesion" is likely to significantly affect the research conclusions of the influence of the fiber's own characteristics on cell behavior in the above literature reports, making it difficult to accurately reveal the relevant cells under the condition of excluding or stripping the interference of the "background adhesion" — Law of interaction of fiber materials
So far, there is no report to examine the diameter of the fiber itself on the basis of excluding the interference of the "background adhesion" (such as the adhesion of cells to the background substrate material through the interfiber space, and the adhesion of cells across multiple adjacent fibers up and down or left and right). , material and its density and other characteristics on cell adhesion and subsequent behavior, the main reason is the lack of effective fiber material platform construction

Method used

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  • Single-layer parallel fiber pattern with cell adhesion contrast characteristic and preparation method thereof
  • Single-layer parallel fiber pattern with cell adhesion contrast characteristic and preparation method thereof
  • Single-layer parallel fiber pattern with cell adhesion contrast characteristic and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0055] A method for preparing a single-layer parallel fiber pattern with contrasting characteristics of cell adhesion, the specific steps are as follows:

[0056] (1) Modify the base:

[0057] The material of the substrate is a gold-sprayed glass sheet, and the modification of the substrate includes the steps of substrate cleaning, graft modification treatment and post-treatment in sequence;

[0058] (1.1) Substrate cleaning: soak the substrate in absolute ethanol for 20 minutes;

[0059] (1.2) Grafting modification treatment: put the cleaned base material into the grafting reagent solution in a closed reaction vessel, use ethanol as a solvent, and react for 2 days under a nitrogen protection atmosphere at 25°C, wherein the grafting reagent is (11-Mercaptoundecyl)hexa(ethylene glycol) (CAS No.: 130727-44-5), the concentration of the grafting reagent solution is 3mM;

[0060] (1.3) Post-treatment: ultrasonically clean the graft-modified substrate with absolute ethanol for 8 min...

Embodiment 2

[0070] A method for preparing a single-layer parallel fiber pattern with contrasting characteristics of cell adhesion, the specific steps are as follows:

[0071] (1) Modify the base:

[0072] The material of the substrate is a glass sheet, and the modification of the substrate sequentially includes the steps of substrate cleaning, plasma treatment, grafting modification treatment and post-treatment;

[0073] (1.1) Substrate cleaning: wipe the substrate with lens tissue, put it in piranha lotion for 30 minutes, and then ultrasonically clean it with ultrapure water;

[0074] (1.2) Plasma treatment: the gas source used is high-purity oxygen, the treatment vacuum is 30Pa, the treatment power is 200W, and the treatment time is 30min;

[0075] (1.3) Grafting modification treatment: put the substrate after plasma treatment into the grafting reagent solution in a closed reaction vessel, use toluene as the solvent and triethylamine as the catalyst, and react under a nitrogen atmosphe...

Embodiment 3

[0082] The preparation method of a single-layer parallel fiber pattern with contrasting cell adhesion characteristics is basically the same as that in Example 2, except that the moving speed of the screw slide table is set to 0.2 cm / min, and the finally prepared pattern with contrasting cell adhesion characteristics A single-layer parallel fiber pattern, including a substrate and a single layer of parallel-arranged fibers fixed on the substrate, the two ends of the single-layer parallel-arranged fibers are fixed on the substrate by polymethacrylic anhydride gelatin, the fiber diameter is 9 μm, adjacent The distance between two fibers is 50 μm (the prepared pattern is as figure 2 The small-diameter densely packed pattern shown in ).

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Abstract

The invention relates to a single-layer parallel fiber pattern with a cell adhesion contrast characteristic and a preparation method thereof. The fiber pattern comprises a substrate and single-layer parallel fibers fixed on the substrate, wherein two ends of the fibers are fixed on the substrate through an adhesive; and the surface of the substrate is grafted and modified by an anti-cell adhesion grafting reagent. The preparation method comprises the following steps of: (1) after grafting and modifying the substrate by adopting the anti-cell adhesion grafting reagent, preparing the single-layer parallel fibers on the surface of the substrate by virtue of an electrostatic spinning, dry spinning, wet spinning or 3D printing method; and (2) coating the adhesive at the combined parts of the two ends of the single-layer parallel fibers prepared in the step (1) and the substrate, and crosslinking and curing the adhesive in a heating curing or illumination curing manner. The fiber pattern prepared by the invention can effectively eliminate interference of various background adhesions, and an effective material platform is provided for accurately revealing the influence of the characteristics such as diameter, material and density arrangement of the fibers on cell behaviors.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and relates to a single-layer parallel fiber pattern with cell adhesion contrast characteristics and a preparation method thereof. The single-layer parallel fiber pattern with cell adhesion contrast characteristics can accurately reveal the diameter, material, and density of the fiber material itself. Potential applications in the influence of features such as arrangement on cell behavior. Background technique [0002] The extracellular matrix in the body provides extremely important biological and physical support for cell adhesion, proliferation, migration, differentiation, and phenotype maintenance. Many important components in the extracellular matrix are fibrous structures, such as collagen, elastin, keratin, etc. Because the fiber material has the characteristics of imitating the extracellular matrix, it has become an important biomaterial to be studied and utilized. The development ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00D04H1/74
CPCC12N5/0068D04H1/74C12N2535/10C12N2533/30C12N2533/40C12N2533/54C12N2533/72C12N2533/80C12N2533/74C12N2533/50
Inventor 姚响顾张弘张耀鹏牛欠欠欧阳秦君
Owner DONGHUA UNIV
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