A photopolymerizable color gel with self-feedback hardness distribution and its preparation method and application

A hardness distribution, self-feedback technology, applied in the direction of material analysis by chemical reaction of materials, material analysis by observing the impact on chemical indicators, etc. Chip complexity and other issues

Active Publication Date: 2022-03-11
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the main challenge in the control and detection of organ chips is that after the assembly is completed, it is a closed system, and conventional detection methods cannot regulate or detect the microenvironment inside the chip through the closed cover or substrate.
In order to realize the detection, it is necessary to design the interface of the detection system on the chip in advance. On the one hand, this increases the complexity of the chip. On the other hand, it is still difficult to detect the spatial distribution of each parameter in the microenvironment in this way.

Method used

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  • A photopolymerizable color gel with self-feedback hardness distribution and its preparation method and application
  • A photopolymerizable color gel with self-feedback hardness distribution and its preparation method and application
  • A photopolymerizable color gel with self-feedback hardness distribution and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Add acrylamide (7.00mg), Bis / PEGDA700 (0.08mg) and photoinitiator 2959 (0.08mg) to water-dispersed silica nanoparticles (90μL, Ф152nm, 30vol%); obtain a prepolymer solution;

[0035] 2) Ultrasonic treatment of the prepolymer solution obtained in 1) for 5 minutes;

[0036] 3) On the basis of 2), the pre-polymerization solution is evenly penetrated into the space between the two parallel glasses separated by capillary force, the gap thickness is 25 μm, and then exposed to the ultraviolet light of λ=365nm for 10s, 30s, 45s, 60s, 120s to photopolymerize the monomer; thus obtain hydrogel films of different colors and hardness;

[0037] 4) In order to increase cell affinity, gelatin was grafted onto the surface of the hydrogel membrane of 3). The hydrogel membranes were then sterilized in 75% ethanol in Petri dishes, in sterile PBS for at least 3 hours and in cell culture medium for 1 hour; the cell culture medium was removed and fresh cell culture medium was injected.

[...

Embodiment 2

[0040] 1) Add acrylamide (10.00mg), Bis / PEGDA700 (0.2mg) and photoinitiator 2959 (0.2mg) to water-dispersed silica nanoparticles (90μL, Ф152nm, 34vol%); obtain a prepolymer solution;

[0041] 2) Ultrasonic treatment of the prepolymer solution obtained in 1) for 5 minutes;

[0042] 3) On the basis of 2), the pre-polymerization solution is evenly penetrated into the space between the two parallel glasses separated by capillary force, the gap thickness is 50 μm, and then exposed to λ=365nm ultraviolet light for 10s, 30s, 45s, 60s, 120s to photopolymerize the monomer; thus obtain hydrogel films of different colors and hardness;

[0043] 4) In order to increase cell affinity, gelatin was grafted onto the surface of the hydrogel membrane of 3). The hydrogel membranes were then sterilized in 75% ethanol in Petri dishes, in sterile PBS for at least 3 hours and in cell culture medium for 1 hour; the cell culture medium was removed and fresh cell culture medium was injected.

[0044] ...

Embodiment 3

[0046] 1) Acrylamide (14.00mg), 7-acryloyloxyamino-4-methylcoumarin (2.00mg), Bis / PEGDA700 (0.45mg) and photoinitiator 2959 (0.45mg) were added to dimethyl ethylene Sulfone-dispersed silica nanoparticles (120 μL, Ф152 nm, 26 vol%); to obtain a prepolymer solution.

[0047] 2) Ultrasonic treatment of the prepolymer solution obtained in 1) for 5 minutes;

[0048] 3) On the basis of 2), the pre-polymerization solution is evenly penetrated into the space between two parallel glasses separated by capillary force, the gap thickness is 75 μm, and then exposed to λ=365nm ultraviolet light for 5 minutes to photopolymerize the monomer ; thereby obtaining a phototunable hydrogel film.

[0049] 4) To increase cell affinity, gelatin was grafted onto the surface of the hydrogel membrane. The hydrogel membranes were then sterilized in 75% ethanol in Petri dishes for at least 3 h in sterile PBS and 1 h in cell culture medium. Remove cell culture medium and inject new cell culture medium. ...

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Abstract

The invention discloses a photopolymerizable gel with color self-feedback hardness distribution and a preparation method thereof. The gel is used for cell supports, and the preparation steps of the gel are as follows: preparing a prepolymerization solution; pouring the prepolymerization solution into two The glass slides placed in parallel were irradiated with 365 nm ultraviolet light to polymerize; during the process, 254 nm and 365 nm ultraviolet light could be used to regulate the mechanical properties of the gel to obtain cell scaffolds with regionalized distribution of mechanical properties. The hardness and distribution of the modified cell scaffold will be displayed in the form of color, and the hardness distribution on the scaffold can be read in situ by taking pictures. This method is applied to a variety of cell culture systems to control the properties of the microenvironment in real time and guide the behavior of cells on it in situ. Without opening the chip and without additional detection equipment, the mechanical parameters of the regulated cell scaffold and the relationship between cell behavior and scaffold properties can be directly obtained through color analysis, which has great application potential.

Description

technical field [0001] The invention relates to an intelligent cell scaffold that can be used for in-situ regulation and sensing of the microenvironment in an organ chip and a preparation method thereof, belonging to the field of biological materials, in particular to a photopolymerizable gel with color self-feedback hardness distribution and a preparation method thereof ,application. Background technique [0002] Organ-on-a-chip is an emerging technology that simulates the functions of human organs through three-dimensional culture of cells in vitro. Organ chips have broad application prospects in the fields of new drug research and development, disease models, personalized medicine and aerospace medicine. In 2016, organ chips were listed as one of the "Top Ten Emerging Technologies" by the World Economic Forum in Davos. One of the most challenging aspects of drug development is how to test the effectiveness and safety of drugs. Cell and animal experiments are currently ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 顾忠泽李森杜鑫
Owner SOUTHEAST UNIV
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