Method for thermally stimulating cultured cells among cells

A cell culture and cell culture technology, applied in the biological field, can solve the problems such as the inability to meet the experimental requirements, the slow heating or cooling speed of the incubator, etc., and achieve the effect of improving the quality and accuracy of scientific research work.

Pending Publication Date: 2020-06-19
广州浚远康生物科技有限公司
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Problems solved by technology

In this regard, the existing conventional incubators cannot meet the experimental requirements (especially the precise thermal stimulation experiments that require instantaneous or short-term)
Although temperature-adjustable incubators have appeared, the heating and cooling speed of this incubator is slow, which cannot meet the needs of instantaneous and precise thermal stimulation for cultured cells.

Method used

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  • Method for thermally stimulating cultured cells among cells
  • Method for thermally stimulating cultured cells among cells

Examples

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Embodiment 1

[0018] Such as figure 1 with figure 2 As shown, the thermal stimulation device includes a water bath 5, an infrared temperature monitoring system 8, a cell culture plate 7, a chassis 6 and a fixed support 7, and the infrared probe 4 of the infrared temperature monitoring system (infrared probe passes through the signal line 1 and The main frame of the infrared temperature monitoring system is connected) is fixed on the fixed bracket 3 by the fixing clip 2, there are at least two bottom frames 6 in the water bath 5, and the bottom frame is provided with a slot for placing a cell culture plate / bottle;

[0019] a. Pre-experiment: Fill sterilized pure water into the water bath 5, and the liquid level is limited to 0.4cm above the upper surface of the bottom frame 6. At this time, the liquid surface is 2-3cm away from the upper edge of the water bath, and one of them The cell culture plate 7 is installed on the slot of the bottom frame, the lower part of the cell culture plate is...

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Abstract

The invention discloses a method for thermally stimulating cultured cells among cells. Firstly, temperature of a water bath is set to be experimental temperature; the water bath is started for heating; when the temperature of the water bath reaches the final experimental temperature, a cell culture plate / flask holding cells which need to be thermally stimulated and a cell culture plate / flask as blank control are respectively placed at slot positions of 2 base frames; the lower part of the cell culture plate / flask is submerged in water, and the upper part of the cell culture plate / flask is opened and is exposed above a water surface; temperature of the inner surface of the cell culture plate / flask as the blank control is monitored by an infrared temperature monitoring system; heating is performed for temperature reaching time, and then heating stimulation is performed for time required for an experiment; and after completion, the cell culture plate / flask holding the cultured cells is quickly taken out, and thus, the precise and instantaneous thermal stimulation experiment of the cultured cells is completed. The method can very well realize precise and instantaneous thermal stimulation treatment on the cultured cells, and can very well meet the experimental needs like accurately observing the influence of thermal stimulation on cell behaviors and functions.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for thermally stimulating cultured cells in an intercellular space. Background technique: [0002] In cell biology research work, cell culture is often required. Conventional cell culture conditions are: place the cell culture dish or flask in the incubator at 37°C, 5% CO 2 cultivated in the environment. Generally, the temperature of the incubator is kept constant at 37°C. For example, during the experiment, in order to achieve specific experimental purposes (such as observing the effect of thermal stimulation on cell behavior, studying the effect of heat-sensitive ion channels or receptors on cell function after heating, etc.), it is necessary to perform thermal stimulation on cultured cells. . In this regard, the existing conventional incubators cannot meet the experimental requirements (especially transient or short-term accurate thermal stimulation exper...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/34
CPCC12M23/12C12M35/00C12M41/22
Inventor 李志远黄荣奇田超汤凤刘雨杰徐浚杰
Owner 广州浚远康生物科技有限公司
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