Method for thermally stimulating cultured cells among cells

Abstract

The invention discloses a method for thermally stimulating cultured cells among cells. Firstly, temperature of a water bath is set to be experimental temperature; the water bath is started for heating; when the temperature of the water bath reaches the final experimental temperature, a cell culture plate / flask holding cells which need to be thermally stimulated and a cell culture plate / flask as blank control are respectively placed at slot positions of 2 base frames; the lower part of the cell culture plate / flask is submerged in water, and the upper part of the cell culture plate / flask is opened and is exposed above a water surface; temperature of the inner surface of the cell culture plate / flask as the blank control is monitored by an infrared temperature monitoring system; heating is performed for temperature reaching time, and then heating stimulation is performed for time required for an experiment; and after completion, the cell culture plate / flask holding the cultured cells is quickly taken out, and thus, the precise and instantaneous thermal stimulation experiment of the cultured cells is completed. The method can very well realize precise and instantaneous thermal stimulation treatment on the cultured cells, and can very well meet the experimental needs like accurately observing the influence of thermal stimulation on cell behaviors and functions.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for thermally stimulating cultured cells in an intercellular space. Background technique: [0002] In cell biology research work, cell culture is often required. Conventional cell culture conditions are: place the cell culture dish or flask in the incubator at 37°C, 5% CO 2 cultivated in the environment. Generally, the temperature of the incubator is kept constant at 37°C. For example, during the experiment, in order to achieve specific experimental purposes (such as observing the effect of thermal stimulation on cell behavior, studying the effect of heat-sensitive ion channels or receptors on cell function after heating, etc.), it is necessary to perform thermal stimulation on cultured cells. . In this regard, the existing conventional incubators cannot meet the experimental requirements (especially transient or short-term accurate thermal stimulation exper...

AI Technical Summary

Problems solved by technology

In this regard, the existing conventional incubators cannot meet the experimental requirements (especially the precise thermal stimulation experiments that require instantaneous or short-term)

Although temperature-adjustable incubators have appeared, the heating and cooling speed of this incubator is slow, which cannot meet the needs of instantaneous and precise thermal stimulation for cultured cells.

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