Traditional Chinese medicine preparation for improving HPV (human papillomavirus) infection symptoms and preparation method thereof

Abstract

The invention discloses a traditional Chinese medicine preparation for improving HPV (human papillomavirus) infection symptoms and a preparation method of the traditional Chinese medicine preparation. The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 40-60 parts of raw coix seeds, 25-35 parts of oldenlandia diffusa, 15-25 parts of anhydridized bovine beta-lactoglobulin, 8-12 parts of curcumin, 10-20 parts of lentinan, 20-30 parts of astragalus membranaceus, 10-20 parts of radix sophorae flavescentis, 10-20 parts of salvia miltiorrhiza, 20-28 parts of rhizoma smilacis glabrae and 4-8 parts of liquorice. According to the invention, through the charge adsorption effect of the anhydridized bovine beta-lactoglobulin and the HPV capsid protein, the broad-spectrum physical blocking of more than 200 HPV types is realized; the curcumin and the lentinan are combined for use, HPV E6 / E7 gene expression is synchronously inhibited, CD8 + T cell immune clearance is activated, the recurrence rate of viruses in the incubation period is reduced, the astragalus membranaceus and the lentinan cooperate to balance Th1 / Th2 immune response, and immune migration is avoided; the raw coix seeds and the rhizoma smilacis glabrae regulate the pH value and flora balance of a genital tract microenvironment and promote skin damage repair, and all the components have a synergistic effect through a'blocking-inhibiting-clearing-repairing 'quadruple mechanism, so that the clinical negative conversion rate can be increased.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine preparation technology, specifically to a traditional Chinese medicine preparation for improving HPV infection symptoms and its preparation method. Background Technology

[0002] HPV belongs to the Papillomaviridae family. It is a spherical, non-enveloped, double-stranded DNA virus with a genome consisting of approximately 8,000 base pairs. It relies on host cells for replication and proliferation. HPV infection is a global public health issue, and it can be effectively prevented through vaccination, safe sexual behavior, and regular screening. Early detection and intervention of precancerous lesions can significantly reduce the incidence of cervical cancer and other malignant tumors.

[0003] HPV infection is a major cause of malignant tumors such as cervical cancer, genital warts, and anal cancer, with more than 600,000 new cases worldwide each year. Persistent infection with high-risk HPV types (such as types 16 / 18) is highly associated with the occurrence of cervical cancer, while low-risk types (such as types 6 / 11) mainly cause genital warts.

[0004] Current treatment options have the following shortcomings:

[0005] Traditional antiviral ingredients (such as matrine and interferon) only target specific HPV subtypes and cannot cover all more than 200 types, especially the combined inhibitory effect on high-risk types 16 / 18 and low-risk types 6 / 11 is insufficient;

[0006] HPV can integrate into the host cell genome through the E6 / E7 oncoprotein. Existing drugs cannot block viral gene expression and latent proliferation, resulting in a high clinical relapse rate.

[0007] Therefore, we propose a traditional Chinese medicine preparation for improving HPV infection symptoms and its preparation method. Summary of the Invention

[0008] The purpose of this invention is to provide a traditional Chinese medicine preparation for improving HPV infection symptoms and its preparation method, thereby solving the problems mentioned in the background art.

[0009] To achieve the above objectives, the present invention provides the following technical solution: a traditional Chinese medicine preparation for improving HPV infection symptoms, comprising the following raw materials in parts by weight:

[0010] 40-60 parts raw coix seed, 25-35 parts oldenlandia diffusa, 15-25 parts acid-anhydrated bovine β-lactoglobulin, 8-12 parts curcumin, 10-20 parts lentinan, 20-30 parts astragalus, 10-20 parts sophora flavescens, 10-20 parts salvia miltiorrhiza, 20-28 parts smilax glabra, and 4-8 parts licorice.

[0011] As a preferred embodiment of the present invention, the raw materials include the following parts by weight:

[0012] 55 parts raw coix seed, 30 parts oldenlandia diffusa, 20 parts acid-anhydrated bovine β-lactoglobulin, 10 parts curcumin, 15 parts lentinan, 25 parts astragalus, 15 parts sophora flavescens, 15 parts salvia miltiorrhiza, 25 parts smilax glabra, and 6 parts licorice.

[0013] As a preferred embodiment of the present invention, the raw materials include the following parts by weight:

[0014] 50 parts raw coix seed, 35 parts oldenlandia diffusa, 18 parts acid-anhydrated bovine β-lactoglobulin, 8 parts curcumin, 12 parts lentinan, 22 parts astragalus, 12 parts sophora flavescens, 12 parts salvia miltiorrhiza, 22 parts smilax glabra, and 5 parts licorice.

[0015] As a preferred embodiment of the present invention, the raw materials include the following parts by weight:

[0016] 45 parts of raw coix seed, 28 parts of oldenlandia diffusa, 22 parts of acid-anhydrated bovine β-lactoglobulin, 12 parts of curcumin, 18 parts of lentinan, 28 parts of astragalus, 18 parts of sophora flavescens, 18 parts of salvia miltiorrhiza, 20 parts of smilax glabra, and 7 parts of licorice.

[0017] This invention also relates to a method for preparing a traditional Chinese medicine preparation for improving HPV infection symptoms, comprising the following steps:

[0018] Step 1: Place 10 kinds of Chinese medicinal herbs, including raw coix seed and white flower tongue grass, in a vacuum freeze dryer at -50℃ with a vacuum degree ≤10Pa and dry for 24-36 hours until the moisture content is ≤5%; use airflow pulverization-ultra-fine pulverization technology, first use 30m / s airflow for preliminary pulverization, and then use a planetary ball mill for ultra-fine pulverization to a particle size ≤5μm, with a mixing uniformity RSD ≤3%;

[0019] Step 2: Dissolve bovine β-lactoglobulin in PBS buffer at pH 8.5 to a concentration of 20 mg / mL, add succinic anhydride at a molar ratio of 1:50, and stir at 37°C for 4 hours while maintaining the pH at 8.0-8.5. Purify the reaction solution and freeze-dry the purified product to obtain a white powder.

[0020] Step 3: Multi-stage extraction: The herbal powder is loaded into the extraction vessel and subjected to supercritical CO2 extraction. Then, it is separated by a separation vessel at a pressure of 8MPa and a temperature of 40℃. The extract is collected, and the extraction residue is added to 8 times the volume of 70% ethanol solution. The mixture is ultrasonically treated at 300W and 25kHz for 30 minutes. After the shiitake mushroom fruiting bodies are crushed, water is added at a material-to-liquid ratio of 1:20, and 500U / g cellulase is added. The mixture is enzymatically hydrolyzed at 50℃ for 2 hours. After boiling to inactivate the enzyme for 10 minutes, the mixture is centrifuged. The supernatant is concentrated to 1 / 5 of its volume, and 95% ethanol is added to a final concentration of 75%. The mixture is allowed to stand at 4℃ for 12 hours, and the precipitate is collected by centrifugation. Then, it is freeze-dried.

[0021] Step 4: Liposome encapsulation: Soy lecithin and cholesterol are dissolved in chloroform at a mass ratio of 4:1, and the mixture is rotary evaporated to form a film. Then, it is hydrated in PBS buffer at pH 6.0. Anhydride-modified protein, curcumin, lentinan, and other active ingredients are added in sequence. The mixture is then ultrasonically emulsified to form a promulgated emulsion. The promulgated emulsion is homogenized in a high-pressure homogenizer at 800 bar for 5 cycles. The particle size distribution is D50 = 100-150 nm. After adjusting the pH to 6.0, the mixture is filtered, dispensed into brown glass bottles, and sealed with nitrogen.

[0022] In a preferred embodiment of the present invention, the mass ratio of curcumin to lentinan is 1:1.2-1.8, which synergistically inhibits HPV E6 / E7 gene expression and latent virus proliferation.

[0023] In a preferred embodiment of the present invention, the raw coix seed is used in combination with Smilax glabra to adjust the pH value of the reproductive tract microenvironment to 4.5-5.5, thereby inhibiting the alkaline conditions required for HPV virus replication.

[0024] In a preferred embodiment of the present invention, the supercritical CO2 extraction conditions are a pressure of 30-40 MPa, a temperature of 45-55°C, and an extraction time of 1.5-2.5 hours.

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0026] This invention achieves broad-spectrum physical blocking of over 200 HPV types through the charge adsorption of anhydride-modified bovine β-lactoglobulin and HPV capsid proteins; the combined use of curcumin and lentinan simultaneously inhibits HPV E6 / E7 gene expression and activates CD8+ T cell immune clearance, which helps reduce the recurrence rate of latent virus; astragalus and lentinan synergistically balance Th1 / Th2 immune responses, avoiding immune shift; raw coix seed and smilax glabra regulate the pH and flora balance of the reproductive tract microenvironment, promoting skin lesion repair. The components work synergistically through a four-fold mechanism of "blocking-inhibition-clearance-repair," which helps improve the clinical seroconversion rate. Attached Figure Description

[0027] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0028] Figure 1 This is a flowchart illustrating the operation of a traditional Chinese medicine preparation for improving HPV infection symptoms and its preparation method according to the present invention. Detailed Implementation

[0029] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0030] Example 1

[0031] formula:

[0032] 50 parts raw coix seed, 30 parts oldenlandia diffusa, 20 parts acid-anhydrated bovine β-lactoglobulin, 10 parts curcumin, 15 parts lentinan, 25 parts astragalus, 15 parts sophora flavescens, 15 parts salvia miltiorrhiza, 24 parts smilax glabra, and 6 parts licorice.

[0033] Preparation method:

[0034] Ten kinds of Chinese medicinal herbs, including raw coix seed and white flower tongue grass, were placed in a vacuum freeze dryer at -50℃ with a vacuum degree ≤10Pa and dried for 24-36 hours until the moisture content was ≤5%. The air jet milling-ultra-micro milling technology was used. The herbs were first initially pulverized with air jets of 30m / s and then ultra-micro milled with a planetary ball mill until the particle size was ≤5μm and the mixing uniformity RSD was ≤3%.

[0035] Bovine β-lactoglobulin was dissolved in PBS buffer at pH 8.5 to a concentration of 20 mg / mL. Succinic anhydride was added at a molar ratio of 1:50. The mixture was stirred at 37°C for 4 hours, maintaining the pH at 8.0-8.5. The reaction solution was then purified, and the purified product was freeze-dried to obtain a white powder.

[0036] Multi-stage extraction: The herbal powder was loaded into an extraction vessel and subjected to supercritical CO2 extraction at a pressure of 35 MPa, a temperature of 50°C, and a time of 2 hours. The extract was then collected in a separation vessel at a pressure of 8 MPa and a temperature of 40°C. The extraction residue was added to 8 times the volume of 70% ethanol solution and ultrasonically treated at 300W and 25kHz for 30 minutes. The shiitake mushroom fruiting bodies were pulverized and water was added at a material-to-liquid ratio of 1:20. 500 U / g cellulase was added and enzymatically hydrolyzed at 50°C for 2 hours. After boiling to inactivate the enzyme for 10 minutes, the mixture was centrifuged. The supernatant was concentrated to 1 / 5 of its volume, and 95% ethanol was added to a final concentration of 75%. The mixture was allowed to stand at 4°C for 12 hours, and then the precipitate was collected by centrifugation and freeze-dried.

[0037] Liposome encapsulation: Soy lecithin and cholesterol were dissolved in chloroform at a mass ratio of 4:1, and the mixture was rotary evaporated to form a film. Then, it was hydrated in PBS buffer at pH 6.0. Anhydride-modified protein, curcumin, lentinan and other active ingredients were added in sequence. The mixture was ultrasonically emulsified to form a colostrum. The colostrum was homogenized in a high-pressure homogenizer at 800 bar for 5 cycles. The particle size distribution D50 = 140 nm was obtained. After adjusting the pH to 6.0, the mixture was filtered, dispensed into brown glass bottles, and sealed with nitrogen.

[0038] Example 2

[0039] formula:

[0040] 40 parts raw coix seed, 25 parts oldenlandia diffusa, 15 parts acid-anhydrated bovine β-lactoglobulin, 8 parts curcumin, 10 parts lentinan, 20 parts astragalus, 10 parts sophora flavescens, 10 parts salvia miltiorrhiza, 20 parts smilax glabra, and 4 parts licorice.

[0041] Preparation method:

[0042] Ten kinds of Chinese medicinal herbs, including raw coix seed and white flower tongue grass, were placed in a vacuum freeze dryer at -50℃ with a vacuum degree ≤10Pa and dried for 24-36 hours until the moisture content was ≤5%. The air jet milling-ultra-micro milling technology was used. The herbs were first initially pulverized with air jets of 30m / s and then ultra-micro milled with a planetary ball mill until the particle size was ≤5μm and the mixing uniformity RSD was ≤3%.

[0043] Bovine β-lactoglobulin was dissolved in PBS buffer at pH 8.5 to a concentration of 20 mg / mL. Succinic anhydride was added at a molar ratio of 1:50. The mixture was stirred at 37°C for 4 hours, maintaining the pH at 8.0-8.5. The reaction solution was then purified, and the purified product was freeze-dried to obtain a white powder.

[0044] Multi-stage extraction: The herbal powder was loaded into an extraction vessel and subjected to supercritical CO2 extraction at a pressure of 30 MPa, a temperature of 45°C, and a time of 1.5 hours. The extract was then collected in a separation vessel at a pressure of 8 MPa and a temperature of 40°C. The extraction residue was added to 8 times the volume of 60% ethanol solution and ultrasonically treated at 250W and 20kHz for 30 minutes. The shiitake mushroom fruiting bodies were pulverized and water was added at a material-to-liquid ratio of 1:20. 400 U / g cellulase was added and enzymatically hydrolyzed at 85°C for 2 hours. After boiling to inactivate the enzyme for 10 minutes, the mixture was centrifuged. The supernatant was concentrated to 1 / 5 of its volume, and 95% ethanol was added to a final concentration of 75%. The mixture was allowed to stand at 4°C for 12 hours, and then the precipitate was collected by centrifugation and freeze-dried.

[0045] Liposome encapsulation: Soy lecithin and cholesterol were dissolved in chloroform at a mass ratio of 4:1, and the mixture was rotary evaporated to form a film. Then, it was hydrated in PBS buffer at pH 6.0. Anhydride-modified protein, curcumin, lentinan and other active ingredients were added in sequence. The mixture was ultrasonically emulsified to form a proemulsion. The proemulsion was homogenized in a high-pressure homogenizer at 700 bar for 4 cycles. The particle size distribution D50 = 120 nm was obtained. After adjusting the pH to 6.0, the mixture was filtered, dispensed into brown glass bottles, and sealed with nitrogen.

[0046] Example 3

[0047] formula:

[0048] 60 parts raw coix seed, 35 parts oldenlandia diffusa, 25 parts acid-anhydrated bovine β-lactoglobulin, 12 parts curcumin, 20 parts lentinan, 30 parts astragalus, 20 parts sophora flavescens, 20 parts salvia miltiorrhiza, 28 parts smilax glabra, and 8 parts licorice.

[0049] Preparation method:

[0050] Ten kinds of Chinese medicinal herbs, including raw coix seed and white flower tongue grass, were placed in a vacuum freeze dryer at -50℃ with a vacuum degree ≤10Pa and dried for 24-36 hours until the moisture content was ≤5%. The air jet milling-ultra-micro milling technology was used. The herbs were first initially pulverized with air jets of 30m / s and then ultra-micro milled with a planetary ball mill until the particle size was ≤5μm and the mixing uniformity RSD was ≤3%.

[0051] Bovine β-lactoglobulin was dissolved in PBS buffer at pH 8.5 to a concentration of 20 mg / mL. Succinic anhydride was added at a molar ratio of 1:50. The mixture was stirred at 37°C for 4 hours, maintaining the pH at 8.0-8.5. The reaction solution was then purified, and the purified product was freeze-dried to obtain a white powder.

[0052] Multi-stage extraction: The herbal powder was loaded into an extraction vessel and subjected to supercritical CO2 extraction at a pressure of 40 MPa, a temperature of 55°C, and a time of 2.5 hours. The extract was then collected in a separation vessel at a pressure of 8 MPa and a temperature of 40°C. The extraction residue was added to 8 times the volume of 80% ethanol solution and ultrasonically treated at 350W and 30kHz for 30 minutes. The shiitake mushroom fruiting bodies were pulverized and water was added at a material-to-liquid ratio of 1:20. 600 U / g cellulase was added and enzymatically hydrolyzed at 95°C for 2 hours. After boiling to inactivate the enzyme for 10 minutes, the mixture was centrifuged. The supernatant was concentrated to 1 / 5 of its volume, and 95% ethanol was added to a final concentration of 75%. The mixture was allowed to stand at 4°C for 12 hours, and then the precipitate was collected by centrifugation and freeze-dried.

[0053] Liposome encapsulation: Soy lecithin and cholesterol were dissolved in chloroform at a mass ratio of 4:1, and the mixture was rotary evaporated to form a film. Then, it was hydrated in PBS buffer at pH 6.0. Anhydride-modified protein, curcumin, lentinan and other active ingredients were added in sequence. The mixture was ultrasonically emulsified to form a colostrum. The colostrum was homogenized in a high-pressure homogenizer at 900 bar for 6 cycles. The particle size distribution D50 = 100 nm was obtained. After adjusting the pH to 6.0, the mixture was filtered, dispensed into brown glass bottles, and sealed with nitrogen.

[0054] Comparative Example

[0055] formula:

[0056] Interferon α-2b 100,000 IU / g, Carbomer 940 1.5%, Glycerin 10%, Sodium Benzoate 0.1%.

[0057] Process:

[0058] The gel matrix was dissolved in a 60°C water bath, and interferon solution (pH 6.0) was slowly added.

[0059] The mixture was homogenized for 30 minutes, filtered (using a 0.45μm filter membrane), and then dispensed.

[0060] The above Examples 1-3 and Comparative Examples were tested, and the data are shown in the table below:

[0061]

[0062]

[0063] In summary, this invention achieves broad-spectrum physical blocking of over 200 HPV types through the charge adsorption of anhydride-modified bovine β-lactoglobulin and HPV capsid proteins; the combined use of curcumin and lentinan simultaneously inhibits HPV E6 / E7 gene expression and activates CD8+ T cell immune clearance, which helps reduce the recurrence rate of latent virus; astragalus and lentinan synergistically balance Th1 / Th2 immune responses, avoiding immune shift; raw coix seed and smilax glabra regulate the pH and flora balance of the reproductive tract microenvironment, promoting skin lesion repair. The components work synergistically through a four-fold mechanism of "blocking-inhibition-clearance-repair," which helps improve the clinical seroconversion rate.

[0064] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or basic characteristics. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within the present invention.

[0065] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A traditional Chinese medicine preparation for improving HPV infection symptoms, characterized in that: Including the following parts by weight of raw materials: 40-60 parts raw coix seed, 25-35 parts oldenlandia diffusa, 15-25 parts acid-anhydrated bovine β-lactoglobulin, 8-12 parts curcumin, 10-20 parts lentinan, 20-30 parts astragalus, 10-20 parts sophora flavescens, 10-20 parts salvia miltiorrhiza, 20-28 parts smilax glabra, and 4-8 parts licorice.

2. The traditional Chinese medicine preparation for improving HPV infection symptoms according to claim 1, characterized in that: Including the following parts by weight of raw materials: 55 parts raw coix seed, 30 parts oldenlandia diffusa, 20 parts acid-anhydrated bovine β-lactoglobulin, 10 parts curcumin, 15 parts lentinan, 25 parts astragalus, 15 parts sophora flavescens, 15 parts salvia miltiorrhiza, 25 parts smilax glabra, and 6 parts licorice.

3. The traditional Chinese medicine preparation for improving HPV infection symptoms according to claim 1, characterized in that: Including the following parts by weight of raw materials: 50 parts raw coix seed, 35 parts oldenlandia diffusa, 18 parts acid-anhydrated bovine β-lactoglobulin, 8 parts curcumin, 12 parts lentinan, 22 parts astragalus, 12 parts sophora flavescens, 12 parts salvia miltiorrhiza, 22 parts smilax glabra, and 5 parts licorice.

4. A traditional Chinese medicine preparation for improving HPV infection symptoms according to claim 1, characterized in that: Including the following parts by weight of raw materials: 45 parts of raw coix seed, 28 parts of oldenlandia diffusa, 22 parts of acid-anhydrated bovine β-lactoglobulin, 12 parts of curcumin, 18 parts of lentinan, 28 parts of astragalus, 18 parts of sophora flavescens, 18 parts of salvia miltiorrhiza, 20 parts of smilax glabra, and 7 parts of licorice.

5. A method for preparing a traditional Chinese medicine preparation for improving HPV infection symptoms, applicable to the traditional Chinese medicine preparation for improving HPV infection symptoms as described in any one of claims 1-4, characterized in that, The methods and steps include the following: Step 1: Place 10 kinds of Chinese medicinal herbs, including raw coix seed and white flower tongue grass, in a vacuum freeze dryer at -50℃ with a vacuum degree ≤10Pa and dry for 24-36 hours until the moisture content is ≤5%; use airflow pulverization-ultra-fine pulverization technology, first use 30m / s airflow for preliminary pulverization, and then use a planetary ball mill for ultra-fine pulverization to a particle size ≤5μm, with a mixing uniformity RSD ≤3%; Step 2: Dissolve bovine β-lactoglobulin in PBS buffer at pH 8.5 to a concentration of 20 mg / mL, add succinic anhydride at a molar ratio of 1:50, and stir at 37°C for 4 hours while maintaining the pH at 8.0-8.

5. Purify the reaction solution and freeze-dry the purified product to obtain a white powder. Step 3: Multi-stage extraction: The herbal powder is loaded into the extraction vessel and subjected to supercritical CO2 extraction. Then, it is separated by a separation vessel at a pressure of 8MPa and a temperature of 40℃. The extract is collected, and the extraction residue is added to 8 times the volume of 70% ethanol solution. The mixture is ultrasonically treated at 300W and 25kHz for 30 minutes. After the shiitake mushroom fruiting bodies are crushed, water is added at a material-to-liquid ratio of 1:20, and 500U / g cellulase is added. The mixture is enzymatically hydrolyzed at 50℃ for 2 hours. After boiling to inactivate the enzyme for 10 minutes, the mixture is centrifuged. The supernatant is concentrated to 1 / 5 of its volume, and 95% ethanol is added to a final concentration of 75%. The mixture is allowed to stand at 4℃ for 12 hours, and the precipitate is collected by centrifugation. Then, it is freeze-dried. Step 4: Liposome encapsulation: Soy lecithin and cholesterol are dissolved in chloroform at a mass ratio of 4:1, and the mixture is rotary evaporated to form a film. Then, it is hydrated in PBS buffer at pH 6.

0. Anhydride-modified protein, curcumin, lentinan, and other active ingredients are added in sequence. The mixture is then ultrasonically emulsified to form a promulgated emulsion. The promulgated emulsion is homogenized in a high-pressure homogenizer at 800 bar for 5 cycles. The particle size distribution is D50 = 100-150 nm. After adjusting the pH to 6.0, the mixture is filtered, dispensed into brown glass bottles, and sealed with nitrogen.

6. The method for preparing a traditional Chinese medicine preparation for improving HPV infection symptoms according to claim 5, characterized in that: The modification rate of the acid-anhydrated bovine β-lactoglobulin is ≥80%, and the IC50 value of its binding to HPV capsid protein is ≤0.8μg / ml.

7. The method for preparing a traditional Chinese medicine preparation for improving HPV infection symptoms according to claim 5, characterized in that: The mass ratio of curcumin to lentinan is 1:1.2-1.8, which synergistically inhibits HPV E6 / E7 gene expression and latent virus proliferation.

8. The method for preparing a traditional Chinese medicine preparation for improving HPV infection symptoms according to claim 5, characterized in that: The combined use of raw coix seed and Smilax glabra can regulate the pH value of the reproductive tract microenvironment to 4.5-5.5, inhibiting the alkaline conditions required for HPV virus replication.

9. The method for preparing a traditional Chinese medicine preparation for improving HPV infection symptoms according to claim 5, characterized in that: The supercritical CO2 extraction conditions are a pressure of 30-40 MPa, a temperature of 45-55℃, and an extraction time of 1.5-2.5 hours.

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