32results about How to "Inhibit maturation" patented technology

The invention relates to a RANKL-TNF sample region mouse monoclonal antibody and its preparation method and use. The RANKL-TNF sample region mouse monoclonal antibody is extracted from a RANKL-TNF sample region mouse monoclonal antibody hybridoma cell strain and has the preservation number of CGMCC NO.6853. The RANKL-TNF sample region mouse monoclonal antibody and its related reagents can effectively neutralize TNF-alpha in vitro thereby inhibiting cell apoptosis caused by TNF-alpha, and neutralize RANKL in vitro thereby inhibiting osteoclast generation. The RANKL-TNF sample region mouse monoclonal antibody can effectively relieve an acute inflammation degree, improve bone mass of a mouse suffering from osteoporosis, improve a cancellous bone trabecula microstructure, and effectively improve osteoporosis symptoms.

The invention provides a special foliar fertilizer for camellia gauchowensis. The special foliar fertilizer is prepared from the following components in parts by weight: 0.2-1.4 parts of urea, 0.1-1.8 parts of monopotassium phosphate, 0.2-1.8 parts of boric acid, 0.1-1.5 parts of magnesium chloride, 0.1-1 part of calcium chloride, 0.02-0.1 part of heteroauxin, 0.01-0.13 part of gibberellin, 0.02-0.15 part of vitamin C, 0.003-0.021 part of naphthylacetic acid and 5000-12000 parts of purified water. Compared with a conventional foliar fertilizer, the foliar fertilizer contains a large quantity of nutrient elements and further is added with medium elements, trace elements and a plant growth regulator, and is capable of improving the fruit setting rate and the fruit retention rate of the camellia gauchowensis fruits, reducing falling flowers and fruits and increasing the output.

The invention provides whitening essence containing bearberry extract lipid nanoemulsion. The whitening essence is prepared from a component A, a component B and a component C, wherein the component A comprises arbutin lipid nanoemulsion, antioxidant biological active peptide, vitamin C and the like; the component B is selected from glycerinum, glycerin monostearate, hexadecyl alcohol, squalane, licoflavone, vitamin E, microcrystalline wax and the like; the component C is selected from avocado oil, silicon oil, vitamin E, stearate, hexadecyl alcohol, Vaseline, beeswax, lanum and the like. The whitening essence does not contain chemical ultraviolet absorbent and is a pure natural cosmetic composition by use of plant whitening agent and biological active peptide as functional components, the bearberry extract based whitening essence can reduce the tyrosinase activity and inhibit melanosome maturity, and can be further effectively combined with licoflavone and biological active peptide, thereby achieving good whitening effect.

The present invention discloses a preservation method for picking, storage, transportation and sales of high-value fruits and vegetables. The method is as follows: when fruits and vegetables reach a physiological maturity period, a pre-picking spraying-type 1-methylcyclopropene preservation agent is sprayed on trees, timely picking or delayed picking is conducted, after sorting, the fruits and vegetables are put into a microenvironmental controlled atmosphere preservation box, after fully pre-cooled in an origin place, the fruits and vegetables are put in a physiological and disease-double-controlling 1-methylcyclopropene preservation bag, and cover locking and storing are conducted. Storage, transportation and sales links have the following three modes: (1) a fresh sales mode: the fruitsand vegetables are directly transported to consumers in a cold chain manner and the fresh sales mode is suitable for e-commerce; (2) a storage mode: the fruits and vegetables are transported to cold storages of a storage and sales place for storage in a whole cold chain manner and the storage mode is suitable for fruit and vegetable storage and sales enterprises; and (3) a sales model: the fruitsand vegetables are divided into full box sales and single basket sales and the sales model is suitable for different consumer groups. The treatment method can delay picking time of the fruits and vegetables, delays occurrence of senescence and decay of the fruits and vegetables, and increases commerciality and sales prices of the fruits and vegetables in the storage, transportation and sales links.

The present invention provides means which can be used for maintaining the freshness of or controlling maturation of plants or perishables such as fruits, vegetables, and flowers and which is safe to the human body. According to the present invention, a method for maintaining the freshness of or conversely controlling the maturation of plants or perishables by controlling the concentration of ethylene gas by use of an ethylene gas adsorbent containing an alcohol extract solution of raw bamboo and a filter having held thereon the alcohol extract solution of raw bamboo. Also, according to the present invention, there is provided an ethylene gas sensor including a quartz crystal microbalance having coated thereon the alcohol extract solution of raw bamboo.

The present invention provides a composition for treating and / or preventing type I diabetes and an application thereof. The active ingredient of the composition is 1.) or 2.) or 3.), as follows: 1.) a mixture of a type I diabetes protein antigen and an immunosuppressor, 2.) a mixture of a type I diabetes protein antigenic epitope polypeptide and an immunosuppressor, 3.) a mixture of a type I diabetes protein antigen, a type I diabetes protein antigenic epitope polypeptide, and an immunosuppressor; the type I diabetes protein antigen is at least one of insulin, glutamic acid decarboxylase, and islet amyloid polypeptide, and the immunosuppressor is at least one of dexamethasone, cyclosporine A, tacrolimus, mycophenolate mofetil, azathioprine, prednisone, early prednisolone, anti-CD4 monoclonal antibody, and anti-CD3 monoclonal antibody.

The invention relates to a humanized antibody targeted to RANKL (Receptor Activator Of Nuclear Factor Kappa B Ligand) and TNF-alpha (Tumor Necrosis Factor) and an application of the humanized antibody. The antibody provided by the invention is obtained by carrying out humanized modification on a region V of a murine monoclonal antibody aiming at RANKL-TNF sample region epitope through a CDR (Complementarity Determining Region) replacement technology. The invention further provides an expression vector for expressing the humanized antibody targeted to RANKL and TNF-alpha and a construction method of the expression vector. The humanized antibody provided by the invention can effectively neutralize TNF-alpha in vitro, inhibit apoptosis caused by TNF-alpha, neutralize RANKL and inhibit generation of osteoclast and meanwhile can antagonize inflammations caused by TNF-alpha and bone destructive effect caused by RANKL. The humanized antibody provides a base for research on novel biological preparations for treating rheumatoid arthritis.

The invention belongs to the field of biological medicines. The invention discloses application of NS1619 for inhibiting activation of NLRP3 inflammatory corpuscles. An experiment finds that the NS1619 can inhibit autooligomerization of a NLRP3 protein, thus the activation of the NLRP3 inflammatory corpuscles is specifically inhibited, and maturation and secretion of inflammatory factors IL-1 betaand IL-18 are inhibited. At the same time, the NS1619 can further alleviate peritonitis in vivo induced by NLRP3 inflammatory corpuscle agonist MSU. Therefore, according to application of NS1619 forinhibiting activation of NLRP3 inflammatory corpuscles, the latest insights are provided for the NS1619 to treat the NLRP3 inflammatory corpuscles related inflammatory diseases and preparing drugs fortreatment of related diseases.

The invention discloses application of EMC10 protein as a biomarker in diagnosis of male infertility. An experiment proves that by adopting substances capable of increasing EMC10 protein activity and / or expression quantity, the male infertility can be prevented, the male infertility can be treated, fertilisation of sperm and eggs is promoted, fertilisation of sperm and zona pellucida-free eggs ispromoted, the mixing ability of the sperm and a cell membrane is improved, sperm dysfunction is treated, sperm maturation is promoted, maturation of the sperm in epididymis is promoted, the motion ability of the sperm is improved, a protein tyrosine phosphorylation level related with capacitation of the sperm is improved, the capacitation of the sperm is promoted, the acrosome reaction ability ofthe sperm is improved, an abnormal form of the sperm is repaired, the activity of sodium / potassium-APT enzyme is recovered, ionic balance in the sperm is maintained, Na<+> balance in the sperm is maintained, HCO3-balance in the sperm is maintained, the balance of a pH value in the sperm is maintained, and the fertility of males is improved. The EMC10 protein disclosed by the invention has an important application value.

The invention provides application of Optineurin as a target point in the preparation of a drug for preventing and treating autoimmune diseases. The drug comprises an antibody specifically combined with an OPTN protein, an Optineurin gene-specific siRNA, an sh-OPTN adeno-associated disease, and an Optineurin small-molecule inhibitor of a steroid derivative shown in the formula (I). The study of the application demonstrates that by inhibiting the Optn gene expression, the maturation of dendritic cells can be inhibited, the polarization of CD4<+>T cells to Th2 and Treg for inflammation suppression is promoted, and the polarization of the CD4<+>T cells to Th1 and Th17 for inflammation promotion is inhibited, so that the autoimmunization system is inhibited, and the effect of treating autoimmune diseases such as multiple sclerosis and rheumatoid arthritis is achieved. The invention discloses a novel role of the Optn gene in immune cells and immune systems, the design and preparation of thedrug for preventing and / or treating the autoimmune diseases by using the Optn gene or protein as the target point and other therapeutic means.

The invention provides use of a hydroxyl polymethoxylated flavonoid compound and / or pharmaceutically acceptable salts and / or a composition formed by the forms at any ratio. The hydroxyl polymethoxylated flavonoid compound can be used as an effective component in a pharmaceutical composition for inhibiting obesity or treating a fatty liver with a scheduled dose to be used for preparing a medicine for inhibiting obesity or used for preparing a medicine for treating the fatty liver, or can be used as a component of a food to be used for preparing a food composition. The hydroxyl polymethoxylated flavonoid compound has the abilities of inhibiting adipose differentiation, inhibiting fat ripening, improving the fatty liver and reducing fat accumulation, and can be used as an effective component of a medicine to achieve the targets of inhibiting obesity or treating the fatty liver. In addition, on the basis that the hydroxyl polymethoxylated flavonoid compound is from a natural plant, and is free of toxicity on cells, the side effect on an individual body is reduced; and furthermore, the hydroxyl polymethoxylated flavonoid compound can be used as the food component to be prepared into daily health food, thus the targets of preventing obesity or treating the fatty liver are achieved.

Disclosed is a protective bag that is formed by depositing a light-reflective material and a photocatalyst material on the outer surface of an air-permeable bag. It is preferable that: indentations are formed on the outer surface of the air-permeable bag, and the light reflective material is a coloured fibre and contains first light-reflective particles having a volume average particle size of 100[mu]m-200[mu]m and second light-reflective particles having a volume average particle size of 1[mu]m-5[mu]m.

The invention discloses multi-effect eye repairing essence and a preparation method thereof. The eye repairing essence is prepared from the following components: a small molecular humectant, undecylenoyl phenylalanine, rhizoma cimicifugae stem cell extract, Alaria extract, Chenodium quinoa extract, EDTA-disodium, sodium carboxymethyl beta-glucan, betaine, a preservative and deionized water. All the components of the eye repairing essence coordinate with one another and gain one another, so that fine wrinkles can be remarkably reduced and a certain curative effect of eliminating dark circles and under-eye puffiness is achieved. The eye repairing essence is mild in property, safe, stable and free of any adverse reaction.

The invention discloses a preparation method and application of a quinoxaline amide compound used as a streptococcus mutans biological membrane inhibitor and relates to the technical field of bacteriostatic drug screening. The inhibitor consists of compounds 2-(4-methoxy-phenyl)-N-(3-{[2-(4-methoxy-phenyl) ethyl] imido}-1,4-dihydro-2-quinoxaline subunit) ethylamine and dimethyl sulfoxide (DMSO). The preparation method comprises the following steps: (1) dissolving 50mg of powdery 2-(4-methoxy-phenyl)-N-(3-{[2-(4-methoxy-phenyl) ethyl] imido}-1,4-dihydro-2-quinoxaline subunit) ethylamine in 1mL of a dimethyl sulfoxide (DMSO) solvent to prepare an inhibitor mother solution of which the initial concentration is 50mg / mL; (2) performing gradient dilution on the inhibitor mother solution by using dimethyl sulfoxide (DMSO), and adding into a brain heart infusion (BHI) liquid culture medium to make sure that the final concentrations of the inhibitor are respectively 0.05mu g / mL, 0.5mu g / mL, 5mu g / mL and 50mu g / mL and the final concentrations of the solvent DMSO are all 1% (v / v). Application tests show that the inhibitor can be used as an oral mouth streptococcus mutans biological membrane inhibitor and a candidate targeted drug for a novel anti-caries strategy.

The invention discloses a strain of bifidobacterium pseudocatenulatum capable of highly utilizing galactooligosaccharide and application of the bifidobacterium pseudocatenulatum, and belongs to the technical field of microorganisms and the technical field of medicines. The bifidobacterium pseudocatenulatum CCFM1170 obtained by screening in the invention can make high use of the galactooligosaccharide, which is specifically embodied in that: after the intervention of the galactooligosaccharide, the relative abundance of the strain in the intestinal tract of a source host is obviously improved; the strain shows stronger applicability, faster growth rate and shorter generation time in an in-vitro YCFA culture medium taking galactooligosaccharide as a carbon source, namely an intestinal microorganism in-vitro simulation culture method; according to the bifidobacterium pseudocatenulatum CCFM1170 disclosed by the invention, the galactooligosaccharide is highly utilized, so that the competitive adaptability of the bifidobacterium pseudocatenulatum CCFM1170 during intestinal colonization is improved; and the colonization strength and durability in the intestinal tract of a mouse are remarkably improved. Therefore, the bifidobacterium pseudocatenulatum CCFM1170 has a huge application prospect in the preparation of a product which has high colonization capability and is used for improving the health of the host.

The invention discloses an application of an oxazolone compound as a streptococcus mutans biofilm inhibitor, which belongs to the field of screening technologies of antibacterial drugs. The chemical name of the compound is 2-(4-chlorphenyl)-4-{[(6-methyl-2-pyridyl) amino] methylene}-1,3-oxazole-5((tetrahydro)-ketone, and an inhibitor formed by dissolving the oxazolone compound in DMSO can inhibit the formation and maturation of streptococcus mutans biofilms. The inhibitor has the advantages of small molecular weight, simple structure, good specificity and the like, can significantly inhibit the formation and maturation of biofilms of streptococcus mutans-a main pathogenic bacterium of caries, but has an unobvious influence on streptococcus mutans in a floating state. The inhibitor has an anti-caries potential and can be used as a candidate targeted drug of novel anti-caries strategies.

The invention discloses application of aspartic acid in preventing or treating obesity. The aspartic acid is L-aspartame, and the usage amount of the L-aspartame is 40-200 microns. The inventor finds that the L-aspartame has the effects of inhibiting adipogenesis, reducing triglyceride level in fat cells, effectively controlling the level of glucose, insulin and free fatty acid in vivo, reducing the content of total fat tissues and fat tissues at each part, and inhibiting fat cell maturation, so that the L-aspartame has potential medicinal value for effectively preventing or treating obesity.

The invention discloses an antibiotic artificial hip joint which comprises acetabulum prosthesis, femoral heads and an artificial hip joint handle (3), wherein a same tube (4) is arranged in the artificial hip joint handle (3), one end of the tube (4) perforates out of the upper part of the artificial hip joint handle (3) and is communicated with exterior, a micropore (2) is arranged on the outer surface of an artificial hip joint and communicated with the tube (4), and the tube (4) is filled with silica sol sustained release agent solution absorbed with antibiotic. The antibiotic artificial hip joint directly inhibits the forming and the maturity of biomembrane, changes the original using way that the antibiotic is used in general veins and partially and externally used, improves the anti-infectious healing efficacy and obviously reduces the toxic reaction and can regularly supplement and replace effective antibiotic and achieve the aims of thoroughly killing bacteria and radically curing infection on the promise of not replacing the prosthesis after operation, thereby not only playing prevention and cure roles to patients, but also curing the infection.

The invention discloses a citrus reiculata Blanco fresh-keeping method, and belongs to the technical field of fruit fresh-keeping, the citrus reiculata Blanco fresh-keeping method comprises the following steps: (1) selecting a sunny day to pick up 50-60% mature citrus reiculata Blanco fruits without pests and diseases, and placing the picked-up citrus reiculata Blanco fruits in a box; (2) washingthe picked-up citrus reiculata Blanco fruits in the step (1) with 20 DEG C to 25 DEG C clear water, and draining the water; (3) placing the citrus reiculata Blanco fruits obtained in the step (2) in abactericide of the temperature of 1 DEG C-5 DEG C, soaking for 30 to 60 minutes, and draining the water; (4) placing the citrus reiculata Blanco fruits obtained in the step (3) in a preservative forsoaking, after the soaking, taking the citrus reiculata Blanco fruits out, and naturally drying in the air; and (5) packing the citrus reiculata Blanco fruits obtained in the step (4) in a fresh-keeping bag or a fresh-keeping box, sealing, and storing at 15 DEG C to 25 DEG C. The citrus reiculata Blanco fresh-keeping method comprising the five steps can minimize the damage to the citrus reiculataBlanco fruits, and preserves the freshness of the citrus reiculata Blanco fruits, and storage and transportation are facilitated.

The invention belongs to the application field of biotechnology and phototherapy and specifically relates to a novel light emitting diode (LED) technology-based method for regulating melanocyte pigment synthesis. Through determined high-purity single spectrum LED light, melanocyte pigment synthesis is regulated, and proliferation and apoptosis of melanocyte are not affected. According to the method, LED yellow light with wavelength of 585+ / -5 nm, light intensity of 18 mw / cm<2> and energy range of 20J / cm<2> is adopted to continuously irradiate melanocyte. Then, tyrosinase activity of melanocyte can be inhibited, melanosome maturation is inhibited to reduce mature pigment granules in the melanocyte III-IV stage, expression of genes and protein related to melanogenesis is inhibited, melanin content is reduced, and proliferation and apoptosis of melanocyte are not affected. The method is safe and effective, can be used for preparation of an in vitro study model of melanocyte, is helpful for making intervening measures for treatment and adjuvant treatment of pigment disorders, and is used for making intervening measures for non-ablative medical cosmetology.

This application discloses a porcine endothelial-derived IL-8 overexpression stable cell line, which was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on July 7, 2016, and the preservation registration number is CGMCC NO. 12682. The application also discloses a preparation method and application of a porcine endothelial-derived IL-8 overexpression stable cell line. The porcine endothelial-derived IL-8 overexpression stably transfected cell line of the present application has good application in the porcine virus immunopathogenic mechanism and the porcine endothelial-derived IL-8 action mechanism, etc. value. The porcine endothelial IL-8 overexpression stable cell line of the present invention also has the effect of anti-inflammation.

The invention discloses dsRNA designed based on a Periplaneta americana Dsx gene, and a preparation method, coding gene and application of the dsRNA. The dsRNA is double-strand RNA consisting of a nucleotide sequence as shown in Seq ID No.1 as a positive-sense strand and a backward complementary nucleotide sequence as shown in Seq ID No.1 as an antisense strand. The dsRNA can be widely applied topreparation of products for controlling periplaneta americana, interfering chemical communication of the periplaneta americana or controlling reproduction of female adult insects of the periplaneta americana or controlling periplaneta americana and other insects having the same gene target sequence. Gene Dsx is used as a target through insect sex, and based on a genetic fragment in the Dsx, the dsRNA is designed. The dsRNA is imported into the bodies of the adult female periplaneta americana, ova can be prevented from generating, and ovaries can be prevented from being mature, so that the reproduction of the female periplaneta americana can be controlled, and the purpose of pest control can be achieved.

The invention relates to the technical field of medicines, in particular to application of a terminalia bellerica extract and terminalia bellerica polyphenol. The invention relates to the applicationof the terminalia bellerica extract to preparation of a drug for treating inflammatory bowel disease and the application of the terminalia bellerica extract to preparation of a drug for inhibiting maturation of dendritic cells. According to the invention, the terminalia bellerica extract and the terminalia bellerica polyphenol can inhibit maturation of the dendritic cells, can effectively relieveinflammatory reaction and have the function of treating enteritis.

The invention provides application of plant extracts containing salidroside and tyrosol to preparation of a medicine for resisting helicobacter pylori and an upper gastrointestinal flora improving agent including the plant extracts, and belongs to the technical field of microorganisms. The preparation method of the plant extracts comprises the following steps: adding an extraction solvent into plant powder; carrying out uniform mixing and ultrasonic extraction to obtain extracting solutions; diluting and centrifuging the extracting solutions; and filtering supernatant liquid through a micro-filtration membrane to obtain a final product. The improving agent is applied to helicobacter pylori-positive people; and improvement of the upper gastrointestinal flora means reduction of the helicobacter pylori in the upper gastrointestine. According to the upper gastrointestinal flora improving agent disclosed by the invention, extracting ratios of the salidroside and the tyrosol in the plant extracts are high, the urease activity can be inhibited in a high-selective manner, and the improving agent has the excellent survival inhibition effect and bacteria removal / discharging effects on the helicobacter pylori and can be applied to the medicine for resisting the helicobacter pylori; and moreover, the prepared improving agent can be used for inhibiting the survival and urease activity of the helicobacter pylori, can maintain the balance of microecology of stomachs, and can improve the upper gastrointestinal flora.

The invention provides application of Optineurin as a target point in the preparation of a drug for preventing and treating autoimmune diseases. The drug comprises an antibody specifically combined with an OPTN protein, an Optineurin gene-specific siRNA, an sh-OPTN adeno-associated disease, and an Optineurin small-molecule inhibitor of a steroid derivative shown in the formula (I). The study of the application demonstrates that by inhibiting the Optn gene expression, the maturation of dendritic cells can be inhibited, the polarization of CD4<+>T cells to Th2 and Treg for inflammation suppression is promoted, and the polarization of the CD4<+>T cells to Th1 and Th17 for inflammation promotion is inhibited, so that the autoimmunization system is inhibited, and the effect of treating autoimmune diseases such as multiple sclerosis and rheumatoid arthritis is achieved. The invention discloses a novel role of the Optn gene in immune cells and immune systems, the design and preparation of thedrug for preventing and / or treating the autoimmune diseases by using the Optn gene or protein as the target point and other therapeutic means.

The invention provides use of a hydroxyl polymethoxylated flavonoid compound and / or pharmaceutically acceptable salts and / or a composition formed by the forms at any ratio. The hydroxyl polymethoxylated flavonoid compound can be used as an effective component in a pharmaceutical composition for inhibiting obesity or treating a fatty liver with a scheduled dose to be used for preparing a medicine for inhibiting obesity or used for preparing a medicine for treating the fatty liver, or can be used as a component of a food to be used for preparing a food composition. The hydroxyl polymethoxylated flavonoid compound has the abilities of inhibiting adipose differentiation, inhibiting fat ripening, improving the fatty liver and reducing fat accumulation, and can be used as an effective component of a medicine to achieve the targets of inhibiting obesity or treating the fatty liver. In addition, on the basis that the hydroxyl polymethoxylated flavonoid compound is from a natural plant, and is free of toxicity on cells, the side effect on an individual body is reduced; and furthermore, the hydroxyl polymethoxylated flavonoid compound can be used as the food component to be prepared into daily health food, thus the targets of preventing obesity or treating the fatty liver are achieved.

The invention discloses application of EMC10 protein as a biomarker in diagnosis of male infertility. An experiment proves that by adopting substances capable of increasing EMC10 protein activity and / or expression quantity, the male infertility can be prevented, the male infertility can be treated, fertilisation of sperm and eggs is promoted, fertilisation of sperm and zona pellucida-free eggs ispromoted, the mixing ability of the sperm and a cell membrane is improved, sperm dysfunction is treated, sperm maturation is promoted, maturation of the sperm in epididymis is promoted, the motion ability of the sperm is improved, a protein tyrosine phosphorylation level related with capacitation of the sperm is improved, the capacitation of the sperm is promoted, the acrosome reaction ability ofthe sperm is improved, an abnormal form of the sperm is repaired, the activity of sodium / potassium-APT enzyme is recovered, ionic balance in the sperm is maintained, Na<+> balance in the sperm is maintained, HCO3-balance in the sperm is maintained, the balance of a pH value in the sperm is maintained, and the fertility of males is improved. The EMC10 protein disclosed by the invention has an important application value.