The invention discloses application of EMC10 protein as a biomarker in diagnosis of male infertility. An experiment proves that by adopting substances capable of increasing EMC10 protein activity and / or expression quantity, the male infertility can be prevented, the male infertility can be treated, fertilisation of sperm and eggs is promoted, fertilisation of sperm and zona pellucida-free eggs ispromoted, the mixing ability of the sperm and a cell membrane is improved, sperm dysfunction is treated, sperm maturation is promoted, maturation of the sperm in epididymis is promoted, the motion ability of the sperm is improved, a protein tyrosine phosphorylation level related with capacitation of the sperm is improved, the capacitation of the sperm is promoted, the acrosome reaction ability ofthe sperm is improved, an abnormal form of the sperm is repaired, the activity of sodium / potassium-APT enzyme is recovered, ionic balance in the sperm is maintained, Na<+> balance in the sperm is maintained, HCO3-balance in the sperm is maintained, the balance of a pH value in the sperm is maintained, and the fertility of males is improved. The EMC10 protein disclosed by the invention has an important application value.