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Bifidobacterium pseudocatenulatum capable of highly utilizing galactooligosaccharide and application of bifidobacterium pseudocatenulatum

A bifidobacterium and viable count technology, applied in the field of microbiology and medicine, can solve the problems of not being able to increase the colonization ability of probiotics, and not being able to enhance the survival or persistence of animal bifidobacteria milk subspecies Bb-12, etc., to achieve Efficient use of galacto-oligosaccharides, increased relative abundance, and fast growth rate

Active Publication Date: 2021-07-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] All of the above have proved that prebiotics, such as galacto-oligosaccharides, can increase the proliferation ability of probiotics in vitro, but reports have shown that the ability of probiotics to colonize in vivo cannot be increased under the condition of supplementing prebiotics, for example , the literature (Alander et al.2001:Effect of galacto-oligosaccharide supplementation onhuman faecal microflora and on survival and persistence of Bifidobacteriumlactis Bb-12 in the gastric testinal tract) discloses: Supplementing galacto-oligosaccharides can not enhance animal Bifidobacterium lactis Bb-12 Survival or persistence of species Bb-12 in the gut

Method used

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  • Bifidobacterium pseudocatenulatum capable of highly utilizing galactooligosaccharide and application of bifidobacterium pseudocatenulatum
  • Bifidobacterium pseudocatenulatum capable of highly utilizing galactooligosaccharide and application of bifidobacterium pseudocatenulatum
  • Bifidobacterium pseudocatenulatum capable of highly utilizing galactooligosaccharide and application of bifidobacterium pseudocatenulatum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1: Screening, strain identification and cultivation of Bifidobacterium pseudocatenulatum CCFM1170

[0073] Specific steps are as follows:

[0074] 1. Differential response of pseudosmall chain Bifidobacterium strains in human intestine after galacto-oligosaccharide intervention

[0075] Recruit 10 subjects (1) have not had abdominal or intestinal surgery in the past 1 year (2) have not used antibiotics (except topical drugs) in the past 2 months (3) have not consumed probiotics / prebiotics / synbiotics in the past 1 month Healthy adults who took supplements took 10 grams of galacto-oligosaccharides per day for 4 weeks. Fecal samples were collected at the baseline period before the experiment, 2 weeks of galacto-oligosaccharide intervention, and 4 weeks of galacto-oligosaccharide intervention, and were immediately stored in a -80°C refrigerator until the fecal DNA extraction experiment. One volunteer used antibiotics during the experimental period, one volunteer d...

Embodiment 2

[0091] Embodiment 2: In the YCFA liquid medium of galacto-oligosaccharide as carbon source, the growth characteristics of pseudo small chain bifidobacterium (Bifidobacterium pseudocatenulatum) CCFM1170

[0092] Specific steps are as follows:

[0093] (1) Streak Bifidobacterium pseudospermum CCFM1170, Bifidobacterium pseudospermum CCFM1046, Bifidobacterium pseudoserinus FJSWXJNDX3M3, Bifidobacterium pseudosmallis FJSWXJNDX9M6, Bifidobacterium pseudoserinus FJSWXJNDX16M1 on mMRS solid culture cultured anaerobically at 37°C for 48 hours to obtain a single colony;

[0094] Pick a single colony and inoculate it in MRS liquid medium, culture it at 37°C for 18 hours for activation, and activate for two generations continuously to obtain an activation solution.

[0095] (2) Take 1mL of activation solution in a sterile centrifuge tube, centrifuge at 5000r / min for 15min, discard the upper medium to obtain the sludge, then resuspend the sludge with 1mL of sterile normal saline, and then...

Embodiment 3

[0099] Embodiment 3: the impact of galacto-oligosaccharides on the colonization ability of pseudo small chain Bifidobacterium (Bifidobacterium pseudocatenulatum) CCFM1170

[0100] Specific steps are as follows:

[0101] Pseudomonas bifidobacterium CCFM1170 and the negative control strain Bifidobacterium pseudosquamium CCFM1046 were respectively inserted into mMRS liquid medium and cultured anaerobically at 37°C for 36 hours, then centrifuged at 5000r / min for 15min at 4°C to collect the sludge, After washing with normal saline, resuspend in normal saline, the bacterial concentration is 5×10 9 CFU / mL, the bacterial suspension was obtained, and the bacterial liquid was prepared and used daily. The dose of galacto-oligosaccharides is 0.4 g / day / mouse, which is dissolved in drinking water according to the average daily water intake of the mice, and the drinking water is changed every 5 days.

[0102] 64 SPF grade BALB / c male mice weighing 20-22g were randomly divided into 8 groups...

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Abstract

The invention discloses a strain of bifidobacterium pseudocatenulatum capable of highly utilizing galactooligosaccharide and application of the bifidobacterium pseudocatenulatum, and belongs to the technical field of microorganisms and the technical field of medicines. The bifidobacterium pseudocatenulatum CCFM1170 obtained by screening in the invention can make high use of the galactooligosaccharide, which is specifically embodied in that: after the intervention of the galactooligosaccharide, the relative abundance of the strain in the intestinal tract of a source host is obviously improved; the strain shows stronger applicability, faster growth rate and shorter generation time in an in-vitro YCFA culture medium taking galactooligosaccharide as a carbon source, namely an intestinal microorganism in-vitro simulation culture method; according to the bifidobacterium pseudocatenulatum CCFM1170 disclosed by the invention, the galactooligosaccharide is highly utilized, so that the competitive adaptability of the bifidobacterium pseudocatenulatum CCFM1170 during intestinal colonization is improved; and the colonization strength and durability in the intestinal tract of a mouse are remarkably improved. Therefore, the bifidobacterium pseudocatenulatum CCFM1170 has a huge application prospect in the preparation of a product which has high colonization capability and is used for improving the health of the host.

Description

technical field [0001] The invention relates to a strain of pseudo-small-chain bifidobacteria with high utilization of galacto-oligosaccharides and an application thereof, belonging to the technical field of microbes and medicine. Background technique [0002] Bifidobacterium pseudosepsis is one of the bifidobacteria commonly found in the intestinal flora of healthy adults. The study found that Pseudomonas bifidobacterium can improve the disease activity index and inflammatory response of mice with chronic, low-grade, systemic inflammation or acute, local ulcerative colitis mice. In addition, it can also produce Or transform folic acid, conjugated linoleic acid, conjugated linolenic acid and other biologically active substances. Bifidobacterium pseudosmall chain may have strong anti-inflammatory function, and has broad application prospects as a potential probiotic. [0003] However, studies have found that with the occurrence of diseases or the growth of age, the content ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/123A23C9/13A23L2/38A23L11/50A23L19/00A23L33/125A23L33/135A61K35/745A61K31/7016A61K31/702A61K31/715A61P1/00A61P1/14C12R1/01
CPCC12N1/20A23L33/135A23L33/125A23L19/00A23L2/382A23C9/1234A23C9/1307A61K35/745A61K31/7016A61K31/702A61K31/715A61P1/00A61P1/14A23V2002/00A23V2200/30A23V2200/3204A61K2300/00Y02A50/30
Inventor 翟齐啸汪姝敏陈卫肖越于雷雷田丰伟陆文伟崔树茂王刚赵建新张灏
Owner JIANGNAN UNIV
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