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Use of hydroxyl polymethoxylated flavonoid compound and/or derivative thereof

A technology of hydroxypolymethoxyflavone and methoxyflavone, which is applied in the field of natural compounds, can solve the problems of insignificant weight loss and achieve the effects of reducing fat accumulation, inhibiting fat differentiation, and inhibiting fat maturation

Active Publication Date: 2015-03-18
GREENYN BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on the mechanism of action of the above-mentioned natural ingredients or natural extracts, it is not directly achieved by regulating the life cycle of fat cells, inhibiting the differentiation of fat cells, and promoting the decomposition of fat cells, so the weight loss effect is relatively insignificant. Therefore, in order to be more Effectively inhibit fat accumulation and obesity, many studies are currently devoted to the development of natural ingredients that can regulate fat cell function or inhibit fat formation

Method used

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  • Use of hydroxyl polymethoxylated flavonoid compound and/or derivative thereof
  • Use of hydroxyl polymethoxylated flavonoid compound and/or derivative thereof
  • Use of hydroxyl polymethoxylated flavonoid compound and/or derivative thereof

Examples

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example 1

[0048] Example 1: Preparation of Hydroxypolymethoxylated Flavonoids

[0049] Take 10 grams of orange peel extract, which contains 40% polymethoxylated flavonoids, dissolve it in 95% ethanol, and then add 3M hydrochloric acid (HCl). The above mixed solution was heated to reflux for 12 hours, and the reaction process was monitored by TLC and LC / MS. After the reaction was completed, it was cooled, and the ethanol was removed with a vacuum device, and ethyl acetate and water were added for extraction. The organic layer extracts were collected, and the aqueous layer extracts were extracted with ethyl acetate and combined. The combined organic extracts were washed with diluted sodium bicarbonate solution, water and brine (30% NaCl) solution, and dried over anhydrous sodium sulfate to remove water. After filtration, concentration under reduced pressure and lyophilization, the obtained light yellow solid is hydroxypolymethoxylated flavonoids.

example 2

[0050] Example 2: 3T3-L1 Preadipocyte Culture

[0051] 3T3-L1 preadipocytes were cultured in DMEM medium containing 10% calf serum, 10,000 units / mL penicillin and 10,000 μg / mL streptomycin, placed in a 10 cm culture dish at 37°C with 5% carbon dioxide cultured in a cell culture incubator. When the cells grow to about 7-8 minutes full, remove the culture medium and rinse with phosphate buffer, add an appropriate amount of trypsin (trypsin-EDTA) at 37°C, and tap the culture plate to remove the cultured cells from the culture At the bottom of the plate, use fresh medium to stop the action of trypsin, use a pipette to pump several times to evenly break up the cells, and then distribute the cells to culture plates of various sizes at a fixed ratio, and place them in 5% Culture in a 37°C cell incubator with carbon dioxide.

example 3

[0052] Example 3: 3T3-L1 Preadipocyte Differentiation Assay

[0053] 3T3-L1 preadipocytes were seeded into a 24-well culture dish, cultured in a DMEM medium containing fetal calf serum for 3 days, and then the medium was replaced with another medium containing fetal calf serum ) in DMEM medium for 2 days and this culture completion day was defined as the 0th day, and the DMI inducer (DEX, MIX, insulin) medium was added on the 2nd day to cultivate for 2 days according to different treatment conditions, in order to make 3T3-L1 preadipocyte differentiation. The medium was then replaced with a DMEM medium containing 10% fetal bovine serum and 5 μg / mL drug INS. After 2 days of culture, the medium was changed on the 4th, 6th and 8th days Prepare a medium containing 10% fetal bovine serum. The cell type of 3T3-L1 preadipocytes was observed every 2 days during the culture process from the 0th day to the 8th day, and the results were as follows: figure 1 A to figure 1 As shown in E...

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Abstract

The invention provides use of a hydroxyl polymethoxylated flavonoid compound and / or pharmaceutically acceptable salts and / or a composition formed by the forms at any ratio. The hydroxyl polymethoxylated flavonoid compound can be used as an effective component in a pharmaceutical composition for inhibiting obesity or treating a fatty liver with a scheduled dose to be used for preparing a medicine for inhibiting obesity or used for preparing a medicine for treating the fatty liver, or can be used as a component of a food to be used for preparing a food composition. The hydroxyl polymethoxylated flavonoid compound has the abilities of inhibiting adipose differentiation, inhibiting fat ripening, improving the fatty liver and reducing fat accumulation, and can be used as an effective component of a medicine to achieve the targets of inhibiting obesity or treating the fatty liver. In addition, on the basis that the hydroxyl polymethoxylated flavonoid compound is from a natural plant, and is free of toxicity on cells, the side effect on an individual body is reduced; and furthermore, the hydroxyl polymethoxylated flavonoid compound can be used as the food component to be prepared into daily health food, thus the targets of preventing obesity or treating the fatty liver are achieved.

Description

technical field [0001] The present invention relates to the use of natural compounds, in particular to the use of a hydroxy polymethoxy flavonoid compound and / or its derivatives. Background technique [0002] At present, the main cause of obesity is that the calorie intake of an individual is higher than the calorie consumption, so that excess energy accumulates in adipose tissue in the form of triglycerides, and then accumulates in the pancreas, liver, skeletal muscle, etc. , promote obesity and metabolic syndrome (metabolic syndrome), such as cardiovascular disease, hypertension, hyperlipidemia, type 2 diabetes, etc. At present, the World Health Organization classifies obesity as a chronic disease, and the results of Taiwan's nutritional health survey from 2004 to 2008 show that the result of excess nutrition will lead to the occurrence of many chronic diseases. [0003] There are many treatment methods for obesity, such as exercise, calorie control, surgery, etc. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/752A23L1/29A61P3/04A61P1/16A61K131/00A23L33/00
CPCA23V2002/00A61K36/752A61K2300/00A23V2200/332A23V2200/32
Inventor 潘敏雄吴嘉利
Owner GREENYN BIOTECH
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