Method for artificially and largely raising Dioryctria pryeri indoors
A kind of artificial technology, applied in the direction of animal husbandry, etc., can solve the problem of no Pseudomonas fruit, and achieve the effect of simple and easy process equipment, low cost, and solving technical bottlenecks.
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Embodiment 1
[0033] 1. Preparation of feed for the fruit spot borer:
[0034] Collect a total of 100 annual healthy pine cones, masson pine cones, and Huashan pine cones. Bring the collected cones back to the laboratory and immerse them individually in paraffin wax with a melting point of 50-60°C. Roll it over, take it out after wrapping evenly, let it cool naturally, put it in a 5°C freezer and refrigerate it for later use.
[0035] 2. Field collection and processing of breeding provenance of the fruit spot borer:
[0036] A total of 118 pupae were obtained by dissecting 200 cones infested by the leaf moth, including 49 males and 69 females. After the sexes were separated, they were cultured in a laboratory with a diameter of 9 cm and 12 pupae in a laboratory with a room temperature of 26±1° C. and a humidity of 75%. A total of 40 males and 51 females emerged normally during the whole cultivation process.
[0037] 3. Feeding and management of adults:
[0038] The newly eclovened adult...
Embodiment 2
[0045] 1. Preparation of feed for the fruit spot borer:
[0046] Collect 80 healthy black pine cones of the same year, bring the collected cones back to the laboratory, immerse them individually in paraffin wax with a melting point of 50-60°C, roll them up and down, left and right, take them out after they are evenly wrapped, and let them cool down naturally. Store in an 8°C freezer for later use.
[0047] 2. Field collection and processing of breeding provenance of the fruit spot borer:
[0048] A total of 127 pupae were obtained by collecting 180 cones harmed by S. japonica and dissecting them indoors, including 61 males and 66 females. After the sexes were separated, they were cultured in a laboratory with a diameter of 9 cm and 10 pupae in a laboratory with a room temperature of 28±1° C. and a humidity of 70%. A total of 49 males and 54 females emerged normally during the whole cultivation process.
[0049] 3. Feeding and management of adults:
[0050] The newly emerge...
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