dsRNA designed based on Periplaneta americana Dsx gene, and preparation method, coding gene and application of dsRNA

Abstract

The invention discloses dsRNA designed based on a Periplaneta americana Dsx gene, and a preparation method, coding gene and application of the dsRNA. The dsRNA is double-strand RNA consisting of a nucleotide sequence as shown in Seq ID No.1 as a positive-sense strand and a backward complementary nucleotide sequence as shown in Seq ID No.1 as an antisense strand. The dsRNA can be widely applied topreparation of products for controlling periplaneta americana, interfering chemical communication of the periplaneta americana or controlling reproduction of female adult insects of the periplaneta americana or controlling periplaneta americana and other insects having the same gene target sequence. Gene Dsx is used as a target through insect sex, and based on a genetic fragment in the Dsx, the dsRNA is designed. The dsRNA is imported into the bodies of the adult female periplaneta americana, ova can be prevented from generating, and ovaries can be prevented from being mature, so that the reproduction of the female periplaneta americana can be controlled, and the purpose of pest control can be achieved.

Description

technical field [0001] The invention relates to the field of sanitation pest control, in particular to dsRNA designed based on the Periplaneta americana Dsx gene, its preparation method, coding gene and application. Background technique [0002] In 1965, a recessive mutation that made the male and female development of offspring intersex was discovered in Drosophila, and this mutant was called doublesex (Dsx) [1] . Studies have shown that the Dsx gene produces a male and a female-specific DSX protein through sex-specific alternative splicing of its transcripts, exerting its bifunctional role in somatic sexual differentiation, thereby regulating downstream related sex-specific traits target gene [2] . Dsx was once thought to be the final master regulatory gene in the sex determination cascade of all insects, however, later studies refuted this claim and considered it a central link between sex determination and sex differentiation [3] . Dsx homologous genes have differen...

AI Technical Summary

Problems solved by technology

By using Dsx as a target to control the reproduction of female Periplaneta americana, and then achieve the purpose of pest control, it will be expected to solve the problems of fast reproduction speed and strong drug resistance of Periplaneta americana in the prior art. However, in the prior art There is no relevant report yet

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