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Applications of lncRNA-MIR3945HG V2 in diagnosis of mycobacterium tuberculosis negative pulmonary tuberculosis

A tuberculosis and tuberculosis technology, applied in the application field of lncRNA-MIR3945HG V2 in the diagnosis of bacterial negative tuberculosis

Pending Publication Date: 2017-03-22
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the detection of pulmonary tuberculosis using lncRNA as a diagnostic marker

Method used

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  • Applications of lncRNA-MIR3945HG V2 in diagnosis of mycobacterium tuberculosis negative pulmonary tuberculosis
  • Applications of lncRNA-MIR3945HG V2 in diagnosis of mycobacterium tuberculosis negative pulmonary tuberculosis
  • Applications of lncRNA-MIR3945HG V2 in diagnosis of mycobacterium tuberculosis negative pulmonary tuberculosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Up-regulated expression of MΦ in MIR3945HG V2 infected with MTB

[0024] Isolation of PBMC from healthy volunteers, immunomagnetic separation of CD14 + For monocytes, GM-CSF was used to induce monocytes to differentiate into MΦ, and the obtained MΦ was infected with MTB. After 72h, real-time fluorescent quantitative PCR was used to detect the expression level of MΦ in MΦ.

[0025] Test results such as figure 1 As shown, it can be seen that the expression level of MΦ in MΦ after MTB infection is significantly up-regulated, indicating that MTB infection promotes the expression of MIR3945HGV2.

Embodiment 2

[0026] Example 2 Detection of expression of MIR3945HG V2 in PBMCs of patients with bacterial negative pulmonary tuberculosis by real-time fluorescent quantitative PCR

[0027] The RNA of PBMCs from 30 patients with bacterium-negative pulmonary tuberculosis and 32 healthy volunteers was extracted, and the expression of lncRNA-MIR3945HGV2 was detected by real-time fluorescent quantitative PCR.

[0028] Test results such as figure 2 As shown, it can be seen that the expression of MIR3945HG V2 in PBMCs of patients with bacterial negative pulmonary tuberculosis showed a significant up-regulation trend (P<0.0001).

Embodiment 3

[0029] Example 3 MIR3945HGV2 as a new target for the diagnosis of bacterial negative pulmonary tuberculosis

[0030] This study found that, compared with healthy people, the expression of MIR3945HG V2 in PBMCs of tuberculosis patients showed a very significant up-regulation trend (P<0.0001). In order to understand whether the expression change of MIR3945HGV2 in PBMC can be used to distinguish healthy people from tuberculosis patients, we used ROC curve to analyze whether the expression change of MIR3945HGV2 in PBMC is used for the diagnosis of tuberculosis.

[0031] According to the relative expression of MIR3945HG V2 in PBMCs of healthy people and tuberculosis patients, statistical analysis software was used to establish a ROC curve with specificity on the abscissa and sensitivity on the ordinate, see image 3 . The results showed that the area under the ROC curve AUC(A)=0.9414, indicating that MIR3945HGV2 has a high accuracy in the diagnosis of tuberculosis patients ( ima...

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Abstract

The invention discloses applications of lncRNA-MIR3945HG V2 in diagnosis of mycobacterium tuberculosis negative pulmonary tuberculosi. The result shows that after MTB is infected with macrophages, the expression of MIR3945HG V2 is up-regulated; the detection result for PBMC of 30 cases of patients suffering from mycobacterium tuberculosis negative pulmonary tuberculosis shows that the expression level of MIR3945HG V2 is obviously higher than that of healthy volunteers; the analysis result for the receiver operating characteristic curve shows that MIR3945HG V2 is expected to become the novel target for diagnosing the mycobacterium tuberculosis negative pulmonary tuberculosis. By detecting the expression level of lncRNA-MIR3945HG V2, the not infected people can be effectively distinguished from the patients suffering from mycobacterium tuberculosis negative pulmonary tuberculosis.

Description

technical field [0001] The present invention specifically relates to the application of lncRNA-MIR3945HGV2 in the diagnosis of bacterial negative pulmonary tuberculosis. Background technique [0002] Tuberculosis is still the infectious disease with the highest infection rate in the world, and the prevention and control situation is very severe. Rapid and sensitive diagnosis is the key to improving the treatment effect and effectively controlling the spread of tuberculosis. Among the pulmonary tuberculosis patients in China, bacterium-negative pulmonary tuberculosis patients account for 60% to 70% of active pulmonary tuberculosis patients, and the clinical symptoms and imaging features of many other lung diseases are difficult to distinguish from tuberculosis. There is a lack of effective diagnostic methods for bacterial-negative pulmonary tuberculosis, which often results in delayed or misdiagnosed patients. Although my country has formulated some diagnostic criteria, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 马骊杨晓帆温茜
Owner SOUTHERN MEDICAL UNIVERSITY
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