Intestinal microflora kit for diagnosis of coronary heart disease and application thereof

A technology of intestinal flora and coronary heart disease, applied in the field of clinical medicine, can solve problems such as intestinal flora kits for diagnosing coronary heart disease that do not yet exist, and achieve great social significance and economic benefits

Inactive Publication Date: 2018-11-06
天津市环湖医院
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

There is no gut microbiota kit for diagnosing coronary heart disease through metabolites

Method used

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Embodiment Construction

[0014] In order to enable those skilled in the art to better understand the technical solutions of the present invention, the present invention will be further described in detail below in conjunction with the best embodiments.

[0015] As shown, an intestinal flora kit for diagnosing coronary heart disease of the present invention is characterized in that it includes the following reagents arranged in sequence:

[0016] The first reagent is a water-soluble primer whose gene sequence is 5'-AGAGTTTGATCATGGCTC-3' and 5'-CCGTCAATTCCTTTGAGTTT-3';

[0017] The second reagent is the pGEM-T easy vector plasmid.

[0018] An application of intestinal flora test kit for diagnosing coronary heart disease, characterized in that:

[0019] a. The genomic DNA of the stool sample is used as a template, and the first reagent is added to water-soluble primers whose gene sequences are 5'-AGAGTTTGATCATGGCTC-3' and 5'-CCGTCAATTCCTTTGAGTTT-3', to High-Fidelity PCR Master Mix with GC Buffer and h...

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Abstract

The invention relates to the field of clinical medical treatment, in particular to an intestinal microflora kit for diagnosis of coronary heart disease and application thereof. A first reagent is a water-soluble primer with gene sequences of 5'-AGAGTTTGATCATGGCTC-3' and 5'-CCGTCAATTCCTTTGAGTTT-3'; a second reagent is a pGEM-T easy vector plasmid. The intestinal microflora kit has the advantages that by performing PCR (polymerase chain reaction) amplification on a metabolism sample, introducing the pGEM-T easy vector plasmid and transferring DH5alpha competent cells, the obviously different organisms exist in the intestines of a healthy volunteer and a patient with the coronary heart disease, and the obvious difference may be used as the feature index for screening the coronary heart disease; after study, a new method is provided for the screening and diagnosis of the coronary heart disease; the great social meaning and economic benefit are realized.

Description

technical field [0001] The invention relates to the field of clinical medicine, in particular to an intestinal flora kit for diagnosing coronary heart disease and its application. Background technique [0002] Coronary heart disease (CHD) is a relatively common disease in the elderly, which seriously affects the quality of life of patients. Although the treatment level of coronary heart disease has been greatly improved in recent years, the treatment effect of most patients is still unsatisfactory, with high mortality and disability rates and poor prognosis. Therefore, early screening and prevention are considered to be the most cost-effective way to control coronary heart disease. [0003] The complex gut microbial ecosystem has been extensively studied in the past few years. We believe that dietary structure will affect the intestinal flora, and conversely, changes in the structure of the intestinal flora will also affect human metabolism, and changes in metabolism have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/04C12N15/11
CPCC12Q1/689
Inventor 朱彧苏文凌赵秀宝于海燕许正旭曹振华
Owner 天津市环湖医院
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