New adipose-derived stem cell technology for plastic surgery and cosmetology

A technology of adipose stem cells and adipocytes, which is applied in the direction of animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve the problems of too much injection volume, adverse consequences, fat liquefaction, etc., and achieve low decay rate and body damage The effect of small, wide-ranging material sources

Inactive Publication Date: 2014-03-26
孙勇
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AI Technical Summary

Problems solved by technology

However, the amount of each injection of this method should not be too much, and complication

Method used

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Embodiment Construction

[0006] Through this example, a new technology of adipose stem cells for plastic surgery of the present invention will be specifically described. The operating steps of the present invention are as follows:

[0007] (1) Collection of adipose tissue. Select healthy slimming volunteers, and use a liposuction machine (abdomen, buttocks, thighs and other fat-rich parts) to extract fat tissue and 60 ml of venous blood under sterile conditions. Wash the adipose tissue with PBS for 2-3 times, put it into the buffer solution containing double antibody (penicillin / streptomycin, each with 1% content), and transport it to the GMP workshop under the condition of 4° in a sterile environment.

[0008] (2) Digestion of adipose tissue. Under the conditions of a 100-grade GMP workshop, the fat particles were shredded, and digested with 0.075% type I collagenase (Sigma-Aldrich, St.Louis, MO) at 37°C for 40 minutes on a constant temperature shaking (low-speed shaking, 50rpm) shaker. The comple...

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Abstract

The invention relates to a method for purifying stem cells by utilizing adipose tissues obtained during the weight-loss liposuction of a healthy volunteer for breast augmentation and wrinkle removal. The method comprises the following operation steps of: (1) collecting the adipose tissues; (2) digesting the adipose tissues; (3) purifying the adipose tissues; (4) purifying adipose-derived stem cells; (5) carrying out amplification culture on the adipose-derived stem cells; (6) freezing the stem cells for storage. The ADSCs (Adipose-Derived Stem Cells) obtained through extraction and purification under the condition that a GMP (Good Manufacturing Practice) workshop is sterile has multiple advantages for the breast augmentation or face wrinkle removal: the ADSCs can be stably proliferated in vitro, is low in mortality rate, less in damage on a machine body during transplantation, wide in material source and good in breast augmentation effect, can achieve ideal wrinkle removing effect through a small quantity of cells, is safe and reliable without rejection and can provide a good method for augmenting breasts and restoring the skin elasticity of adolescence.

Description

technical field [0001] The invention is a new technology for preparing adipose stem cells from adipose tissue for plastic surgery such as breast enlargement and wrinkle removal, and relates to the fields of medical biotechnology and plastic surgery science. Background technique [0002] When human beings enter the 21st century, people's most pursuits, especially female comrades, are gradually shifting from materialistic desires to health and beauty. A good figure is undoubtedly a woman's natural capital. A plump breast and a plump and elastic face are very attractive to both women and men. However, some women are born with breast atrophy or become smaller or loose and sagging with age, and have no elasticity. [0003] At present, there are three types of breast augmentation methods: prosthesis breast augmentation, injection breast augmentation and autologous fat transplantation. Prosthetic breast augmentation is a breast prosthesis made of silicone jelly or hydrogel wrappe...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
Inventor 孙勇
Owner 孙勇
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