The invention discloses a compound natural plant extract with a spot-removing function and a preparation method and an application thereof. The compound natural plant extract is prepared by the following raw materials by mass: 50-100 parts of mulberry leaves, 30-60 parts of licorice, 10-50 parts of scutellaria, and 1-35 parts of bearberries. According to the invention, extraction is performed by ultrasonic wave extraction technology which shortens the extraction time, prevents damage of effective components caused by high-temperature heating; the obtained compound natural plant extract can effectively remove aged cutin, lightened spots from the inside to the outside of the body, provide sufficient moisture to maintain skin metabolism, accelerate the spot-removing process, has significant antioxidation effect during spot removal, can remove free radicals in the body, protect skin cells not to be influenced excessively by oxidized free radicals, alleviate skin aging, and eliminate the generation of face wrinkles. Through tests, the compound natural plant extract has no toxic or side effect, and is applicable to the preparation of high-efficient and safe spot-removing wrinkle-resistant cosmetics.