Method for scaled production of radix astragali hairy roots

A hairy root and large-scale technology, applied in the field of astragalus biotechnology cultivation, can solve the problems of astragalus reduction, heavy metal pollution, and pesticide reduction in cultivated varieties, and achieve the effect of short production cycle and uniform production quality

Pending Publication Date: 2020-06-05
内蒙古自治区生物技术研究院
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  • Application Information

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Problems solved by technology

[0003] With the strengthening of people's growing health care concept, the demand for Astragalus membranaceus will increase year by year, but at present, the wild resources of Astragalus membranaceus are decreasing year by year, and the cultivated varieties are declining, and they are facing problems such as pesticide pollution and heavy metal residues.

Method used

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  • Method for scaled production of radix astragali hairy roots
  • Method for scaled production of radix astragali hairy roots
  • Method for scaled production of radix astragali hairy roots

Examples

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Embodiment 1

[0059] A method for large-scale production of astragalus hairy roots, comprising the following steps:

[0060] Step 1. Prepare Astragalus aseptic vaccine

[0061] Soak Astragalus mongolica seeds in tap water for 24 hours, soak in 70% ethanol for 3 minutes, rinse with sterile water for 5 times; pour 1% mercuric chloride solution for 10 minutes; rinse with sterile water for 5 times; inoculate until pre-sterilized cultured on MS medium at a temperature of 25°C and a humidity of 60%, to obtain aseptic Astragalus seedlings;

[0062] Step 2. Strain activation and cultivation

[0063] Add Agrobacterium ATCC15834 into liquid YEB medium and cultivate at 26°C at -250rpm until the OD600 value is 0.6; then, culture the bacteria in 1 / 2MS liquid medium at 26°C and 150rpm for 30min;

[0064] Step 3. Material pre-cultivation and co-cultivation

[0065] The leaves of the aseptic seedlings are transferred to the MS medium that adds 0.3mg / L of the phytohormone IBA, cultured in the dark for 2 ...

Embodiment 2

[0072] A method for large-scale production of astragalus hairy roots, comprising the following steps:

[0073] Step 1. Prepare Astragalus aseptic vaccine

[0074] Soak the Astragalus membranaceus seeds in tap water for 20 hours, soak them in 70% ethanol for 2 minutes, rinse them with sterile water for 5 times, pour them into 1% mercuric chloride solution and soak them for 8 minutes, rinse them with sterile water for 5 times, and inoculate them until they are extinguished in advance. Bacteria on the MS medium, temperature 30 ℃, humidity 60% culture, to obtain Astragalus aseptic vaccine;

[0075] Step 2. Strain activation and cultivation

[0076] Add Agrobacterium LBA9402 into liquid YEB medium and cultivate at 26°C and 220rpm until the OD600 value is 0.5; then, culture the bacteria liquid in 1 / 2MS liquid medium and cultivate at 28°C and 180rpm for 30min;

[0077] Step 3. Material pre-cultivation and co-cultivation

[0078] The petiole of the aseptic seedling was transferred ...

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Abstract

The invention provides a method for scaled production of radix astragali hairy roots. The method comprises the steps of preparing a radix astragali bacteria-free strain, activating and culturing agrobacterium, pre-culturing and co-culturing hairy roots, and performing extended culturing on the hairy roots. The radix astragali hairy roots prepared by the method have high astragaloside iv content, and besides, the method has the advantages that based on guaranteeing the content of the astragaloside iv, stable high-yield radix astragali hairy roots can be realized, the production cycle is short,the production quality is unified, and the method is suitable for normal-temperature growth conditions.

Description

technical field [0001] The invention relates to a method for large-scale production of hairy roots of Astragalus membranaceus, belonging to the field of Astragalus membranaceus biotechnology cultivation technology. Background technique [0002] Astragalus is a perennial herb belonging to the leguminous family, and its roots have been used as medicine with a history of more than 2,000 years. The main pharmacological effects of Astragalus include invigorating qi and strengthening the surface, diuresis, detoxification and expulsion of pus, and promoting muscle growth. It is mainly used to treat shortness of breath, collapse, palpitations, spontaneous sweating, physical weakness and edema, chronic nephritis, dehydration, chronic diarrhea, qi deficiency, and insufficient qi and blood. , Sinking of Qi, has antibacterial and anti-inflammatory, antihypertensive and diuretic, detoxifying and choleretic effects, and is a commonly used Chinese herbal medicine. [0003] With the streng...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N15/82A01H5/06A01H6/00
CPCA01H4/001A01H4/005A01H4/008C12N15/8205C12N15/8261
Inventor 张秀娟白朕卿随洋田雨吴佳文高新新陈启渊张宇森
Owner 内蒙古自治区生物技术研究院
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