Epitope polypeptide applicable to mycobacterium tuberculosis infection detection and application thereof
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A technology of mycobacterium tuberculosis and antigenic epitopes, applied in the fields of peptides, biological tests, antibacterial drugs, etc., can solve the problems of high cost, unsuitability, and inapplicability for widespread promotion
Active Publication Date: 2012-06-27
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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[0006] However, due to the protection of patent rights, the cost of introducing related products for clinical diagnosis in my country is extremely high and it is not suitable for the special epidemiological situation in my country, and it is not suitable for widespread promotion, and the diagnosis using ESAT-6 and CFP-10 as antigens It is difficult for reagent development to escape the patent protection of these two antigens
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Embodiment 1
[0023] Embodiment 1 can be used for the antigen that Mycobacterium tuberculosis detects
[0024] Mycobacterium tuberculosis specific antigen Rv3615c, its amino acid composition is shown in SEQ ID NO.10.
[0028] Example 2 Polypeptides that can be used for Mycobacterium tuberculosis detection
[0029] The amino acid sequence of the polypeptide is a polypeptide composed of 8-11 consecutive amino acids in the antigen Rv3615c, as shown in the following table, preferably such as SEQ-ID-NO.1, SEQ-ID-NO.2, SEQ-ID-NO .5, shown in SEQ-ID-NO.6, SEQ-ID-NO.7.
[0030] Table 1. Epitope peptide information:
[0031]
Embodiment 3
[0032] Embodiment 3 antigen is applied to the development of detection kit
[0033] The antigen protected by this patent can be used for the diagnosis of Mycobacterium tuberculosis infection, forming a kit for clinical or laboratory diagnosis.
[0034] The test kit contains:
[0035] 1. A mixed polypeptide peptide library composed of one or several polypeptides in Example 2;
[0036] 2. Phytohemagglutinin (PHA) solution positive control stimulus;
[0037] 3. ELISPOT96-well PVDF membrane plate;
[0038] 4. Human γ-interferon monoclonal antibody (primary antibody), biotin-labeled human γ-interferon monoclonal antibody (secondary antibody), horseradish peroxidase-labeled streptavidin and horseradish peroxidation enzyme reaction substrate;
[0039] 5. Other reagents and consumables required for ELISPOT detection.
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Abstract
The invention discloses certain mycobacterium tuberculosis specific antigen epitope polypeptides, and particularly discloses an antigen Pv3615c antigen epitope polypeptide consisting of 8-11 continuous polypeptide fragments. The clinical detection practicability of the antigen polypeptide is evaluated according to the reaction sensitivity and specificity of the antigen polypeptide in a tuberculosis case. As proved by a result, an antigen polypeptide has high T cell reactivity in a tuberculosis patient, and the positive rate is up to 64 percent (9 / 14). Compared with the result of a healthy volunteer, the antigen epitope polypeptide has the specificity of up to 100 percent (6 / 6). Mycobacterium tuberculosis infection can be diagnosed effectively by applying an antigen peptide library specific T cell gamma interferon.
Description
technical field [0001] The present invention relates to Mycobacterium tuberculosis specific antigen epitope polypeptide, especially the antigen epitope polypeptide of antigen Rv3615c. Background technique [0002] Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. According to WHO estimates, one-third of the world's population is infected by Mycobacterium tuberculosis. There are more than nine million new cases of active infection and more than one million deaths each year. In recent years, the incidence of tuberculosis in my country has remained high, with more than one million new cases and nearly 200,000 deaths each year, which has seriously harmed the health of our citizens, social stability and economic development. [0003] Early diagnosis of tuberculosis is of great significance in controlling the progression of the disease in patients and the spread of the disease. At present, the clinical diagnosis of tuberculosis still lacks high-sensitivi...
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