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Marker for digestive system cancer detection and application thereof

A technology for the detection of digestive system and cancer, applied in the field of biomedicine, can solve problems such as undisclosed and early gastric cancer

Active Publication Date: 2020-03-27
BEIJING FRIENDSHIP HOSPITAL CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3. There are few studies on MRI imaging in the evaluation of early gastric cancer. With its good tissue resolution, MRI is more and more used for staging examination of tumors, and it is generally not used for screening of early gastric cancer.
[0008] However, none of the above prior art discloses the proportion of Vimentin+CD11b+ cells, the proportion of CD16+ cells, and the proportion of CD11c+ cells as a marker for gastric cancer

Method used

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  • Marker for digestive system cancer detection and application thereof
  • Marker for digestive system cancer detection and application thereof
  • Marker for digestive system cancer detection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Using CyTOF to detect the proportion of cells positively expressing Vimentin, CD11b, CD16, and CD11c in paired cancer tissues, paracancerous tissues, and peripheral blood samples of gastric cancer patients

[0052] 1. Collect matched cancer tissue, paracancerous tissue and peripheral blood samples from 35 patients with gastric cancer undergoing surgical resection, and collect peripheral blood from 20 healthy volunteers.

[0053] 2. Preparation of single cells:

[0054] 2.1 Preparation of peripheral blood single cells

[0055] (1) Take 2ml of peripheral blood, equilibrate and centrifuge at room temperature, remove the serum by suction, add 1:1 PBS to the remaining blood cells to dilute and mix well;

[0056] (2) Take another 15ml centrifuge tube, add 2ml of Ficoll lymphocyte separation solution, and slowly add the whole blood diluted in step (1) to Ficoll with a disposable pipette to make it in a suspended state;

[0057] (3) Balance the centrifuge tube in st...

Embodiment 2

[0095] Example 2 Application of proportion of cells positive for Vimentin, CD11b, CD16, and CD11c in gastric cancer screening and pathological staging of gastric cancer

[0096] 1. Blood collection

[0097] Collect 2ml of whole blood from the individual to be tested, store it in a vacuum blood collection tube with EDTA as the anticoagulant, and send the sample to the laboratory as soon as possible after blood drawing (the process from blood drawing to PBMCs preparation should not exceed 24 hours).

[0098] 2. Preparation of peripheral blood single cells.

[0099] Single cells were prepared according to the preparation method of peripheral blood single cells in Example 1.

[0100] 3. Cell staining for optical flow cytometry:

[0101] (1) Take out the PBMC cell cryopreservation medium in the freezing condition of -80°C, and preheat it to a fluid state;

[0102] (2) Transfer the PBMC cells in step (1) to a centrifuge tube, add MACS to resuspend, transfer to a flow tube, balanc...

Embodiment 3

[0112] Example 3 The application of the proportion of Vimentin, CD11b, CD16, and CD11c positive expression cells in the evaluation of gastric cancer treatment effect and prognosis evaluation of gastric cancer

[0113] 1. Collect 2ml of whole blood from gastric cancer patients before and after treatment, and store them in vacuum blood collection tubes with EDTA as anticoagulant. Send the sample to the laboratory as soon as possible after blood drawing (the process from blood drawing to PBMCs preparation should not exceed 24 hours);

[0114] 2. Carry out flow cytometry according to the method for embodiment 2;

[0115] 3. Result analysis:

[0116] According to flow analysis results, see Figure 5 and Image 6 , Before gastric cancer patients received treatment, the proportion of Vimentin+CD11b+ cells was greater than 10%; after gastric cancer patients received treatment, the proportion of Vimentin+CD11b+ cells decreased significantly, down to less than 5%; the change in the p...

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Abstract

The invention discloses a marker for digestive system cancer detection and application of the marker. The marker is a combination of Vimentin and CD11b. The expression conditions of the marker in different individuals show the special phenomena of ultrahigh expression in peripheral blood of a cancer patient, higher expression in peripheral blood of an early cancer patient and almost no expressionin peripheral blood of a healthy volunteer, and the marker can be used for digestive system tumor early screening and solid tumor treatment dominant population including gastric cancer and evaluatingthe treatment effect.

Description

technical field [0001] The present invention belongs to the field of biomedicine, and more specifically relates to a marker for cancer detection, especially a marker for cancer screening, cancer stage determination, cancer treatment effect evaluation or cancer prognosis evaluation. Background technique [0002] China is a country with a high incidence of gastric cancer. The incidence rate of gastric cancer in the natural population is about 31.28 / 100,000, of which the incidence rate of gastric cancer is 42.93 / 100,000 in men and 19.03 / 100,000 in women. The age-standardized incidence rate of men is twice that of women The mortality rate of gastric cancer in my country is about 21.48 / 100,000, and both the incidence rate and the mortality rate rank the second among malignant tumors. Early gastric cancer has no obvious symptoms, and most patients are already in the middle and advanced stages when they are first diagnosed, missing the best time for treatment. The 5-year survival r...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCC07K14/47C07K14/70553G01N33/57446G01N33/68G01N33/6848G01N2333/70553
Inventor 李琴蒋晓月杜琪魏雨涵范学科张栋
Owner BEIJING FRIENDSHIP HOSPITAL CAPITAL MEDICAL UNIV
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