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Application of CD1c to smear and culture negative pulmonary tuberculosis diagnosis

A tuberculosis, bacteria-negative technology, applied in the application field of CD1c in the diagnosis of bacteria-negative pulmonary tuberculosis

Active Publication Date: 2016-03-16
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no report on the diagnosis of lung-related diseases including tuberculosis using the expression level of CD1c in peripheral blood mononuclear cells (PBMC) as an index.

Method used

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  • Application of CD1c to smear and culture negative pulmonary tuberculosis diagnosis
  • Application of CD1c to smear and culture negative pulmonary tuberculosis diagnosis
  • Application of CD1c to smear and culture negative pulmonary tuberculosis diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 The expression of CD1C in the DC infected with MTB is lowered

[0021] PBMC of separation of healthy volunteers, immune magnetic beads assigned CD14 + Monuclear cells use GM-CSF and IL-4 to induce monocytes to differentiate the DC and infect the obtained DC MTB.After 72H, real -time fluorescence quantitative PCR detects the expression level of CD1C in DC.

[0022] The test results are like figure 1 It can be seen from this that the expression level of the CD1C infected by MTB infection is significantly reduced, indicating that MTB infection inhibits the expression of CD1C.

Embodiment 2

[0023] Example 2 The expression of CD1C in DC in patients with a tuberculosis of bacteria yin and lung tuberculosis

[0024] PBMC of Patients and Healthy Volunteer Patients and Healthy Volunteers, immunomagnetic beads are divided into CD14 + Monuclear cells use GM-CSF and IL-4 to induce single-core cells to differentiate to DC. After 72H, real-time fluorescent quantitative PCR detection CD1C expression.

[0025] The test results are like figure 2 It can be seen from this that the expression level of CD1C in DC in patients with villain tuberculosis is significantly lower than that of healthy volunteers ( P <0.05).

Embodiment 3

[0026] Example 3 The expression of CD1C in PBMC in patients with a tuberculosis of bacteria yin and lung tuberculosis

[0027] The PBMC of 119 patients with germine -lung tuberculosis and health volunteers, flow cell detection cell surface CD1C expression.

[0028] The test results are like image 3 It can be seen from this that the PBMC of the cell surface expression of the cells of bacteria and lung tuberculosis is 0.9%, which is obviously lower than that of health volunteers (9.2%), such as image 3 Show A; the results of the average fluorescent strength detection also show that the fluorescence strength expressed in CD1C in PBMC patients in PBMC in PBMC P image 3 Shohs shown; indicating that the expression level of CD1C in PBMC in PBMC in the aids of bacteria is significantly lower than that of health volunteers.

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Abstract

The invention discloses application of CD1c to smear and culture negative pulmonary tuberculosis diagnosis. It is found that after MTB is infected with DCs, CD1c expression is down-regulated; in DCs coming from PBMCs of a smear and culture negative pulmonary tuberculosis patient, compared with a healthy volunteer, the expression level of CD1c is remarkably down regulated; the detection result of PBMCs of 119 smear and culture negative pulmonary tuberculosis patients also shows that the expression level of CD1c is remarkably lower than that of a healthy volunteer; the receiver operation characteristic curve analysis result shows that it is hopeful that CD1c becomes a new target spot of smear and culture negative pulmonary tuberculosis diagnosis. By detecting the express level of CD1c mRNA, non-infected people and smear and culture negative pulmonary tuberculosis patients can be effectively differentiated.

Description

Technical field [0001] The invention involves the application of CD1C in the diagnosis of bacterial yin tuberculosis. Background technique [0002] Tuberculosis is still the highest infectious disease in the global scope, and the prevention and control situation is very severe.Fast and sensitive diagnosis is the key to improving the effect of treatment and effectively controlling the spread of tuberculosis.Among the national tuberculosis patients, 60%to 70%of patients with active tuberculosis patients with mobilizer tuberculosis, and the clinical symptoms and imaging characteristics of multiple other lung diseases are difficult to distinguish between tuberculosis.The lack of effective diagnosis of bacterial yin and lung tuberculosis often causes patient diagnosis or misdiagnosis.Although my country has developed several standards for diagnosis, there are still many confusions in clinical applications, and it has not solved the problem of effective diagnosis of fungal yin and lung...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12R1/01
CPCC12Q1/6851C12Q2531/113C12Q2563/107
Inventor 马骊温茜
Owner SOUTHERN MEDICAL UNIVERSITY
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