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Application of cd1c in the diagnosis of bacterial-negative pulmonary tuberculosis

A tuberculosis, bacteria-negative technology, applied in the application field of CD1c in the diagnosis of bacteria-negative pulmonary tuberculosis

Active Publication Date: 2018-09-25
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there is no report on the diagnosis of lung-related diseases including tuberculosis using the expression level of CD1c in peripheral blood mononuclear cells (PBMC) as an index

Method used

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  • Application of cd1c in the diagnosis of bacterial-negative pulmonary tuberculosis
  • Application of cd1c in the diagnosis of bacterial-negative pulmonary tuberculosis
  • Application of cd1c in the diagnosis of bacterial-negative pulmonary tuberculosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Down-regulation of CD1c expression in DCs infected with MTB

[0021] Isolation of PBMC from healthy volunteers, immunomagnetic separation of CD14 + Monocytes were induced with GM-CSF and IL-4 to differentiate into DCs, and the resulting DCs were infected with MTB. After 72 hours, the expression level of CD1c in DC was detected by real-time fluorescent quantitative PCR.

[0022] Test results such as figure 1 As shown, it can be seen that the expression level of CD1c in DC after MTB infection is significantly reduced, indicating that MTB infection inhibits the expression of CD1c.

Embodiment 2

[0023] Example 2 Down-regulation of CD1c expression in DCs of bacterium-negative pulmonary tuberculosis patients

[0024] Isolation of PBMCs from bacterium-negative pulmonary tuberculosis patients and healthy volunteers, immunomagnetic separation of CD14 + For monocytes, GM-CSF and IL-4 were used to induce monocytes to differentiate into DCs. After 72 hours, real-time fluorescent quantitative PCR was used to detect the expression of CD1c.

[0025] Test results such as figure 2 As shown, it can be seen that the expression level of CD1c in the DCs of bacteria-negative pulmonary tuberculosis patients was significantly lower than that of healthy volunteers ( P <0.05).

Embodiment 3

[0026] Example 3 Down-regulation of CD1c expression in PBMCs of patients with bacterium-negative pulmonary tuberculosis

[0027] PBMCs from 119 bacterium-negative pulmonary tuberculosis patients and healthy volunteers were isolated, and the expression of CD1c on the cell surface was detected by flow cytometry.

[0028] Test results such as image 3 As shown, it can be seen from it that the PBMCs expressing CD1c on the cell surface of bacterium-negative pulmonary tuberculosis patients were 0.9%, which was significantly lower than that of healthy volunteers (9.2%), as shown in image 3 Shown in A; the average fluorescence intensity test results also show that the fluorescence intensity of CD1c expression in PBMCs of bacterium-negative pulmonary tuberculosis patients is significantly lower than that of healthy volunteers ( P image 3 Shown in B; it shows that the expression level of CD1c in PBMC of patients with bacterial negative pulmonary tuberculosis is significantly lower th...

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Abstract

The invention discloses application of CD1c to smear and culture negative pulmonary tuberculosis diagnosis. It is found that after MTB is infected with DCs, CD1c expression is down-regulated; in DCs coming from PBMCs of a smear and culture negative pulmonary tuberculosis patient, compared with a healthy volunteer, the expression level of CD1c is remarkably down regulated; the detection result of PBMCs of 119 smear and culture negative pulmonary tuberculosis patients also shows that the expression level of CD1c is remarkably lower than that of a healthy volunteer; the receiver operation characteristic curve analysis result shows that it is hopeful that CD1c becomes a new target spot of smear and culture negative pulmonary tuberculosis diagnosis. By detecting the express level of CD1c mRNA, non-infected people and smear and culture negative pulmonary tuberculosis patients can be effectively differentiated.

Description

technical field [0001] The invention relates to the application of CD1c in the diagnosis of bacterial negative pulmonary tuberculosis. Background technique [0002] Tuberculosis is still the infectious disease with the highest infection rate in the world, and the prevention and control situation is very severe. Rapid and sensitive diagnosis is the key to improving the treatment effect and effectively controlling the spread of tuberculosis. Among the pulmonary tuberculosis patients in China, bacterium-negative pulmonary tuberculosis patients account for 60%-70% of active pulmonary tuberculosis patients, and the clinical symptoms and imaging features of many other lung diseases are difficult to distinguish from tuberculosis. There is a lack of effective diagnostic methods for bacterial-negative pulmonary tuberculosis, which often results in delayed or misdiagnosed patients. Although my country has formulated some diagnostic criteria, there are still many confusions in clinic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/689C12Q1/04C12R1/01
CPCC12Q1/6851C12Q2531/113C12Q2563/107
Inventor 马骊温茜
Owner SOUTHERN MEDICAL UNIVERSITY
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