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Urine pathogenic bacteria detection system

A pathogenic bacteria detection, pathogenic bacteria technology, applied in the field of urine pathogenic bacteria detection system, can solve the problem of low detection rate of pathogenic bacteria

Pending Publication Date: 2021-09-10
PEKING UNIV FIRST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The embodiment of the present invention provides a urine pathogenic bacteria detection system, which at least solves the problem of low detection rate of pathogenic bacteria in rUTI patients with negative urine culture methods by conventional urine culture methods

Method used

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Effect test

application example 1

[0064] An 83-year-old male patient had recurrent urinary tract infection (rUTI) after urological intubation and bladder perfusion. Negative urine culture. "Pseudomonas aeruginosa" is identified through the detection system of the present invention, and the bacterial count is 50cfu / ml. After the drug susceptibility test, it is relieved after clinically giving targeted treatment. The above colonies grew as Figure 4 As shown, the report results are shown in Table 2.

[0065] Table 2. Comparison table between the report results of the existing urine culture method and the report results of the detection system of the present invention.

[0066]

[0067]

application example 2

[0069] A 77-year-old female patient was clinically diagnosed with rUTI and her urine culture was negative. After being cultivated by the detection system of the present invention for 4 days, it is found that hemolytic colonies can be seen on the anaerobic blood agar plate, and the mass spectrometry is identified as Prevotella two-way, and the bacterial count is 10 3 cfu / ml, classified as pathogenic bacteria with potential pathogenic significance, after the drug susceptibility test, it was relieved after clinical targeted treatment. Colonies grow as Figure 5 shown. Continue to cultivate until 7 days, no other pathogenic bacteria grow. The report results are shown in Table 3.

[0070] Table 3. Comparison table between the report results of the existing urine culture method and the report results of the detection system of the present invention.

[0071]

[0072] From the above application examples, it can be seen that routine urine culture may lead to false negative resu...

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Abstract

The invention discloses a urine pathogenic bacteria detection system, and relates to the field of pathogenic bacteria detection equipment. The system comprises urine sample collection equipment, urine culture equipment and pathogenic bacteria identification equipment. The urine sample collection equipment is used for collecting a urine sample of a urine culture negative patient meeting an inspection standard; the urine culture equipment is used for culturing various pathogenic bacteria in the urine sample of the urine culture negative patient under proper culture conditions; and the pathogenic bacteria identification equipment is used for identifying various pathogenic bacteria cultured by the urine culture equipment to obtain an identification result of the urine culture negative patient. The system can increase the detection rate of pathogenic bacteria of patients suffering from urine culture negative urinary tract infection.

Description

technical field [0001] The invention relates to the field of pathogen detection equipment, in particular to a urine pathogen detection system. Background technique [0002] Urinary tract infection (Urinary Tract Infection, UTI) is one of the most common types of infection in humans, more common in adult women, about 50% of women have at least one UTI in their lifetime, and at least 30% of them are relieved after appropriate antibiotic treatment Recurrence within one year (Recurrent UTI, rUTI). [0003] Generally, positive indicators such as leukocyte esterase and nitrite in routine urine examination suggest UTI, but they lack specificity, and negative indicators of these indicators cannot exclude UTI. Therefore, the current laboratory diagnosis of UTI still mainly relies on urine culture. [0004] The basic steps of urine culture are as follows: take a clean mid-section urine sample, inoculate 1 μl or 10 μl, inoculate on blood agar plate medium and a screening medium for G...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/04C12M1/00
CPCC12Q1/045C12Q1/18
Inventor 黄磊林连君郑波李湘燕孙立颖李海霞
Owner PEKING UNIV FIRST HOSPITAL
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