A plasma exosome miRNA biomarker and its application

A biomarker, exosome technology, applied in the field of medicine, can solve the problems of invasive operation technology, loss of information, requirements and other problems, and achieve high sensitivity and specificity, high sensitivity and good specificity.

Active Publication Date: 2022-07-05
ATTACHED OBSTETRICS & GYNECOLOGY OSPITAL MEDICALCOLLEGE ZHEJIANG UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to cervical stenosis, biopsy is risky, invasive and sometimes technically demanding
In addition, it has been shown in previous studies that endometrial cancer has intratumoral heterogeneity, which means that in biopsy, some valuable information may be lost, such as some tumor subtypes

Method used

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  • A plasma exosome miRNA biomarker and its application
  • A plasma exosome miRNA biomarker and its application
  • A plasma exosome miRNA biomarker and its application

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Embodiment Construction

[0020] The present invention will be further described below through specific embodiments, and the following description is only for explaining the present invention, and the present invention is not limited thereto.

[0021] The specific screening and preparation process of a plasma exosome miRNA biomarker and its application in the preparation of a kit for screening and diagnosis of endometrial cancer provided by the present invention are as follows:

[0022] (1) 56 peripheral blood samples and pathological information were collected (including 31 healthy volunteers and 25 endometrial cancer patients).

[0023] (2) The peripheral blood collected with the blood collection tube containing ACD anticoagulant was centrifuged at 1900×g for 10 minutes at 4°C within 8 hours of blood collection, and then the light yellow supernatant was aspirated, and centrifuged at 16000×g at 4°C for 10 minutes. Take the upper layer, i.e. plasma, and distribute it into 1.5ml nucleic acid-free EP tub...

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Abstract

The invention discloses a plasma exosome miRNA biological marker and application thereof. The miRNA biological marker includes one or more of miR-15a-5p, miR-106b-5p, and miR-107, and has Application in the preparation of a kit for screening and diagnosing endometrial cancer. In 202 tested samples, the expression of 3 exosomal miRNAs could distinguish endometrial cancer patients from healthy volunteers (AUC=0.873), and it could also be combined with tumor markers CEA and CA125 (AUC=0.904 ) to achieve higher sensitivity and specificity. At the same time, these three miRNAs also play a role in early diagnosis (diagnosis of patients with endometrial cancer), and their combined AUC value in the ROC curve is 0.869, which can be further improved to 0.915 when combined with tumor markers. The kit prepared by the invention is composed of specific amplification primers and general PCR amplification reagents, has high clinical value, and can be effectively used for screening and diagnosis of endometrial cancer, including early screening.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a plasma exosome miRNA biomarker and its application in preparing a kit for screening and diagnosis of endometrial cancer. Background technique [0002] Cancer has always been a major public health problem in China and the world. Endometrial cancer is a common type of gynecological cancer, and its morbidity and mortality are increasing year by year. According to Globocan 2018, endometrial cancer ranks second among gynecologic tumors in both incidence and five-year prevalence in China. Approximately 73,253 new cases were diagnosed in 2018. Risk factors for endometrial cancer include age, obesity, increased menstrual cycles, a high-fat diet, and genetics. The current gold standard for the diagnosis of endometrial cancer is still tissue biopsy. However, because of cervical stenosis, biopsy is risky, invasive and sometimes technically demanding. In addition, endometrial cancer ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178C12Q2600/166
Inventor 陆燕刘鹏渊吕卫国周兰韵
Owner ATTACHED OBSTETRICS & GYNECOLOGY OSPITAL MEDICALCOLLEGE ZHEJIANG UNIV
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