60Co-Gamma radiation method for breeding new wheat high-molecular-weight glutenin subunit line

A technology of glutenin subunit and high molecular weight, which is applied in the field of breeding new strains of wheat high molecular weight glutenin subunit by 60Co-γ radiation method, can solve the problems that have not been seen before, and achieve the improvement of breeding quality, improvement of breeding quality, and breeding high efficiency effect

Inactive Publication Date: 2012-06-27
ANHUI SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] After extensive search of patent documents and publications around the world, no use has been found. 60 Report on Co-γ Irradiation Breeding of Wheat High Molecular Weight Glutenin Subunit HMW-GS Mutant Lines

Method used

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  • 60Co-Gamma radiation method for breeding new wheat high-molecular-weight glutenin subunit line
  • 60Co-Gamma radiation method for breeding new wheat high-molecular-weight glutenin subunit line
  • 60Co-Gamma radiation method for breeding new wheat high-molecular-weight glutenin subunit line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] (1) Take 100 grains of Chongqing bread wheat dried seeds, put them into a nylon mesh bag, and place 60 Under Co-γ rays, the radiation dose rate is adjusted to 200Gy energy level, irradiated for 10 minutes, and taken out after standing for 48 hours.

[0069] (2) Plant M 1 generation seeds. 15 centimeters of land ditch depth, 20 centimeters of row spacing, 10 centimeters of plant spacing, 43 strains of seedlings, 1 fringe seed of every plant harvest after maturity, totally 1204 seeds, mix, constitute mutagenesis 2 generations (M 2 ) seed population.

[0070] (3) Plant M 2 generation seeds. Planting method is the same as M 1 In this generation, 1204 plants were planted, 915 plants became seedlings, and a single plant was harvested after maturity, a total of 915 plants, and 5 grains were randomly selected from each plant for sodium dodecyl sulfate-polyacrylamide SDS-PAGE electrophoresis.

[0071] (4) Extraction of wheat M by half-grain method 2 Gluten:

[0072] ① Pr...

Embodiment 2

[0106] (1) Take 100 dried seeds of Heke University No. 2, put them into a nylon mesh bag, and place 60 Under Co-γ rays, the radiation dose rate was adjusted to 200Gy, irradiated for 10 minutes, and taken out after standing for 48 hours.

[0107] (2) Plant M 1 generation seeds. The depth of ditching in the land is 15 cm, the distance between rows is 20 cm, and the distance between plants is 10 cm. There are 51 seedlings. After maturity, each plant harvests 1 panicle seed, and a total of 1173 seeds are mixed to form the second generation of mutagenesis. 2 seed group.

[0108] (3) Plant M 2 generation seeds. Planting method is the same as M 1 Generation, 1173 plants were planted, 903 plants became seedlings, and a single plant was harvested after maturity, a total of 903 plants, 5 grains were randomly selected from each plant for sodium dodecyl sulfate-polyacrylamide SDS-PAGE electrophoresis.

[0109] (4) Extraction of wheat M by half-grain method 2 gluten.

[0110] ① Pre...

Embodiment 3

[0139] (1) Take 100 dried seeds of Heke University No. 2, put them into a nylon mesh bag, and place 60 Under Co-γ rays, the radiation dose rate was adjusted to 200Gy, irradiated for 10 minutes, and taken out after standing for 48 hours.

[0140] (2) Plant M 1 generation seeds. The depth of ditching in the land is 15 centimeters, the row spacing is 20 centimeters, seeds are sown on demand, the plant spacing is 10 centimeters, and 51 seedlings are grown. After maturity, each plant harvests 1 ear seed, a total of 1173 seeds, mixed to form the second generation of mutagenesis M 2 seed group.

[0141] (3) Plant M 2 generation seeds. Planting method is the same as M 1 Generation, 1173 plants were planted, 903 plants became seedlings, and a single plant was harvested after maturity, a total of 903 plants.

[0142] (4) planting M 3generation seeds. 17 embryonic half seeds (M 3 ), the planting method is the same as M 1 .

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Abstract

The invention belongs to a wheat radiation breeding method, in particular to a 60Co-Gamma radiation method for breeding a new wheat high-molecular-weight glutenin subunit line. Reports on the 60Co-Gamma ray breeding of a new wheat high-molecular-weight glutenin subunit mutant line in the prior art are not seen. The method includes the following steps: wheat seeds are irradiated by 60Co-Gamma rays for ten minutes, and the dosage rate is 200Gy; a first mutagenized generation is produced; seeds of a second mutagenized generation are produced; individuals of the M2 generation are grown and harvested; the wheat glutenin of the M2 generation is extracted by the half-seed method, and protein electrophoresis is carried out; half seeds are grown, and a third mutagenized generation is harvested; the wheat glutenin of the M3 generation is extracted; M3 protein electrophoresis is carried out; and the new mutant line with different number of high-molecular-weight glutenin subunit bands or high-molecular-weight glutenin subunit bands at different positions is determined by comparing with the parents. The method has the advantages that: the method breeds the new wheat high-molecular-weight glutenin subunit mutant line by combining 60Co-Gamma ray radiation with the electrophoresis and combining the field planting with indoor electrophoresis screening; and the result is accurate, scientific and reliable.

Description

technical field [0001] The invention belongs to the method for wheat radiation breeding, specifically 60 Breeding of new wheat lines with high molecular weight glutenin subunits by Co-γ radiation method. Background technique [0002] Wheat seed storage protein is an important food plant protein, and its output accounts for 1 / 3 of all cereal crops food plant protein. Wheat storage proteins are composed of 70% gliadins and 30% glutenins. Glutenin mainly exists in the form of glutenin macropolymer GMP, which is composed of high molecular weight glutenin subunit HMW-GS and low molecular weight glutenin subunit LMW-GS connected and polymerized by disulfide bond S-S. The content and properties of glutenin macropolymer GMP are closely related to the quality of wheat flour. The composition and quality of high molecular weight glutenin subunit HMW-GS affect the formation of GMP, and then affect the quality of flour. [0003] The high molecular weight glutenin subunit HMW-GS is enc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06G01N27/447
Inventor 张从宇王敏张怡婷刘纯利林平崔广荣
Owner ANHUI SCI & TECH UNIV
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