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Method for improving culturing efficiency of japonica rice anther

A technology of anther and japonica rice, which is applied in the field of improving the efficiency of japonica rice anther culture, can solve the problems of low seedling rate, callus induction rate and green seedling differentiation rate, etc. The effect of reproductive efficiency

Active Publication Date: 2013-12-25
赫聪
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AI Technical Summary

Problems solved by technology

[0002] Anther culture is an effective and rapid means of stabilizing mutated materials. Since the research on rice anther culture and breeding in the 1970s, anther culture has developed into a mature and practical auxiliary method for rice breeding; but at the same time, anther culture has callus Disadvantages of low tissue induction rate and green seedling differentiation rate, and low seedling rate

Method used

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  • Method for improving culturing efficiency of japonica rice anther
  • Method for improving culturing efficiency of japonica rice anther
  • Method for improving culturing efficiency of japonica rice anther

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Embodiment Construction

[0025] A method of improving the efficiency of Japonica rice anther culture of the present invention comprises the following steps:

[0026] (1) Preparation of mother liquor of japonica rice anther medium

[0027] The medium mother liquor used in anther culture mainly includes four parts: mother liquor A, mother liquor B, mother liquor C, mother liquor D, and also includes some plant growth regulators. The following table shows its main components and their concentrations:

[0028] Table 1: Mother liquor A: macroelements, calculated on the basis of 10 times the concentration

[0029]

[0030] 10 times the concentration of MS medium mother solution in Table 1 was used as the control group. 10 times the concentration of mother solution A refers to the concentration of working solution of mother solution A after diluting mother solution A 10 times with water.

[0031] Table 2 Mother liquor B: Trace elements, calculated at 100 times concentration

[0032]

[0033]

[...

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Abstract

The invention discloses a method for improving the culturing efficiency of japonica rice anther. The method comprises the steps that firstly, culture medium mother solutions of the japonica rice anther are prepared according to formulas of the culture medium mother solutions of the japonica rice anther, then the various mother solutions are utilized to prepare an induction culture medium, a differentiation culture medium and a rooting culture medium all of which are used in different periods, and the culture media in the different periods are correspondingly applied to induction, green plantlet differentiation and rooting of calluses of the japonica rice anther respectively. By the adoption of the method, the percentage of seeding emergency of culturing of the japonica rice anther can be improved, the rice breeding progress is quickened, and the seed selection efficiency of good variety of rice is improved.

Description

technical field [0001] The invention relates to the field of japonica rice anther culture, in particular to a method for improving the efficiency of japonica rice anther culture Background technique [0002] Anther culture is an effective and rapid means of stabilizing mutated materials. Since the research on rice anther culture and breeding in the 1970s, anther culture has developed into a mature and practical auxiliary method for rice breeding; but at the same time, anther culture has callus The disadvantages of low tissue induction rate and green seedling differentiation rate, and low seedling rate. Contents of the invention [0003] In order to overcome the problems and deficiencies in the prior art, the first object of the present invention is to provide a mother liquor of japonica rice anther culture medium and induction medium, differentiation medium and rooting medium prepared from the mother liquor. [0004] Another object of the present invention is to provide a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 余波林添资龚红兵景德道周义文盛生兰李闯钱华飞曾生元
Owner 赫聪
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