Microbial seal-healing capsule for coal mine air leakage plugging as well as preparation method and application of microbial seal-healing capsule
A microbial and self-healing technology, applied in the field of coal mine air leakage plugging materials, can solve the problems of secondary cracking of cracks, spontaneous combustion of coal in goafs, failure to detect and repair in time, etc., to achieve the effect of prolonging life and uniform repair.
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Embodiment 1
[0027] Example 1: The microbial spores used in this example are spores of Bacillus coriolis, and the selected wall material is at least one of epoxy resin, melamine-formaldehyde resin, urea-formaldehyde resin, polyurethane, polyvinyl alcohol and cellulose acetate butyrate The main material of the selected plugging air is cement material.
[0028] Weigh 5g of inosine, 25g of yeast powder, 5g of hydroxypropyl methylcellulose, and 150g of microcrystalline cellulose, and mix them evenly. At the same time, take 150g of water and add 3g of dry spore powder to make it evenly dispersed, and then weighed 150g Calcium nitrate is dissolved in water with spores dispersed, and then the liquid solution is added to the evenly mixed inosine, yeast powder, hydroxypropyl methyl cellulose, microcrystalline cellulose, and stirred evenly to make it into a cluster. The mixture is slowly added to the multifunctional granulator for extrusion, selected from a mesh plate of 40 mesh, and the cooling system...
Embodiment 2
[0031] Embodiment 2: The microbial spores used in this embodiment are at least one of Bacillus pasteurii, Bacillus sphaericus, Bacillus sphaericus, Bacillus kokeli, Bacillus pseudostrongy, Bacillus subtilis, and nitrifying bacteria The selected wall material is at least one of porous lightweight aggregate, sodium alginate, silica gel, clay particles, diatomaceous earth, and ceramsite, and the selected main material for plugging air is fly ash foam material.
[0032] Weigh 3 g of yeast powder, 5 g of peptone, and 1000 mL of deionized water to prepare a microbial culture medium, and adjust the pH to 9.0 by adding NaOH solution dropwise. After autoclaving at 121°C for 30 minutes and cooling to room temperature, inoculate Bacillus pasteurianus and add 30 mL of 4mol / L urea solution. Incubate with shaking at 30°C with an oscillation frequency of 170r / min for 24h.
[0033] The porous lightweight aggregate is passed through a 0.075mm sieve and washed with water, with a particle size rangi...
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