The invention provides an online sub-pixel optical component damage detection method based on radiation calibration, which relates to the field of online optical component detection, and in particular relates to a quick online detection method of a large-caliber optical component of a large optical system. The online detection method can solve the problem that the high-precision size of a damage in an online detection picture cannot be acquired by an existing online detection method, and comprises the following steps: 1, establishing a sample set; 2, estimating the probability epsilon of a non-outlier of the sample set; 3, randomly extracting M groups of small samples from the sample set D; 4, establishing a series of LSSVM (least squares support vector machine) regression models f(W1),..., f(Wm),..., f(WM) by a cross validation method; and 5, selecting a proper error evaluation function Z(x) through the sample set D to verify the regression models f(W1),..., f(Wm),..., f(WM) one by one, thus acquiring the high-precision sub-pixel size of the whole damage area. The method can be applied to the field of online optical component detection.