The invention discloses a device and a method for aligning a pinhole of a point-diffraction interferometer, belonging to the technical field of optical precise debugging, namely a device and a method for aligning the pinhole of the point-diffraction interferometer provided to realize real-time monitoring of precise aligning and aligning states of a focusing spot and a diffraction pinhole in the point-diffraction interferometer. The device comprises a laser device, a laser beam expander, a first dispersion prism, a half-wave plate, a second dispersion prism, a third dispersion prism, a quarter-wave plate, a focus lens, a pinhole plate, a three-dimensional scanning and micro-adjusting mechanism, a first laser power meter, a second laser powder meter, a third laser power meter, a position detector and a computer. According to the device and the method disclosed by the invention, the aligning speed is increased; the pinhole aligning state is quickly aligned again when being monitored; power change of the laser device is detected by a sole laser power meter in the invention and the aligning state is reflected by a ratio of the laser power instead of an absolute value so that error judgment on the aligning state of the pinhole caused by laser power change is overcome.