The invention provides a microbial sand-fixing dust depressor and a preparation method thereof. The microbial sand-fixing dust depressor comprises the following components in percentage by mass: 0.8-2.5% of microbial powder, 3.0-5.0% of urea, 8.1-10.1% of calcium chloride and the balance of deionized water, wherein the microorganism in the microbial sand-fixing dust depressor is bacillus pasteurii powder. The preparation method comprises the following steps: proportionally mixing the calcium chloride with the deionized water, stirring till the calcium chloride is completed dissolved, adding the urea, and after the urea is dissolved, adding the bacillus pasteurii powder till the bacillus pasteurii powder is completely dissolved, thereby obtaining a mixed solution, namely the microbial sand-fixing dust depressor. Compared with the traditional inorganic sand-fixing agent, the microbial sand-fixing dust depressor is high in permeability; compared with the traditional organic sand-fixing agent, the microbial sand-fixing dust depressor has the characteristics of high ageing resistance, simplicity in preparation, convenience in construction and the like. Proved by desert sample tests, the sand-fixing dust depressor is high in ageing resistance and wind erosion resistance and has unconfined compressive strength higher than 1MPa. Proved by raise dust sample tests, the sand-fixing dust depressor has a good dust fixing effect.