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Novel beauveria bassiana granules

A technology of Beauveria bassiana and granules, which is applied in the direction of insecticides, biocides, animal repellants, etc., and can solve the problems of instability, slow effect of large-scale control of fungal insecticide pests, industrialization, and standardized formulation processing Difficulties and other problems, to achieve the effect of high-efficiency infection and high-efficiency insect resistance

Inactive Publication Date: 2016-11-23
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Beauveria bassiana has faced some problems in production so far. One of the most important problems is that the effect of fungal insecticides on large-scale control of pests is slow and unstable, which leads to difficulties in industrialization, standardization and formulation processing. , affecting the promotion of biologics
In addition, when applied in the field, the field environment is mostly low humidity and high temperature environment, which is not conducive to the germination of spores and the growth of mycelium to infect insects. Factors such as temperature, humidity and light time will make the application of Beauveria bassiana in the field and its effect play a role. Subject to greater restrictions

Method used

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  • Novel beauveria bassiana granules
  • Novel beauveria bassiana granules
  • Novel beauveria bassiana granules

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1: preparation Beauveria bassiana granule

[0024] 1) Preparation of PD liquid medium: Peel and chop potatoes, weigh 1000g, add 4000m deionized water, boil for 30min, filter to remove residue, add 75g glucose to the filtrate, and steam sterilize under high pressure for 20min.

[0025] 2) Configure PDA plate medium: peel and chop potatoes, weigh 1000g and add 4000m deionized water, boil for 30min, filter to remove residue, add 75g glucose to the filtrate, add 100g agar powder, steam sterilize under high pressure for 20min, After cooling to 50°C, the culture medium was introduced into a sterile petri dish in a sterile operating bench.

[0026] 3) Inoculate Beauveria bassiana on PDA plate medium, and culture at 25° C. for 7 days.

[0027] 4) Prepare fermentation medium: add 20g glucose, 2g potassium dihydrogen phosphate, 1g magnesium sulfate, 0.5g zinc sulfate, 40mg vitamin B to 5L liquid PD medium 1 , 5000g corn stalk granules and 6L sterile water, mix well. ...

Embodiment 2

[0031] Embodiment 2: Effect experiment of Beauveria bassiana granule

[0032] 1. Plant bacteria application

[0033] It was carried out in the experimental field of Yitong Plant Protection Station, Kaoshan Town, Yitong. The corn cultivar was Xianyu 335, which was planted in two rows on a large ridge. One ridge was a test group, and 40 corn plants were randomly selected in the test group. Dig a 10-15cm deep pit near the root of the plant during the period, place about 125g of Beauveria bassiana GZ01 granule in the pit, make the granule contact with the root of the corn plant, and then gently cover the pit with a layer of soil.

[0034] 2. Inoculation of plants

[0035] After the artificially reared adult worms of Ostrinia oleatus lay eggs, cut off the wax paper with egg masses and put them into a moisturizing 1.5mL ventilated centrifuge tube. Put a standard egg mass (about 60 eggs) in each centrifuge tube and place them in a constant temperature incubator. Cultivate (26±1°C),...

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Abstract

The invention discloses beauveria bassiana granules. The beauveria bassiana granules are prepared through a method comprising the following steps that a fermentation culture medium is inoculated with beauveria bassiana or beauveria bassiana spores, wherein the fermentation culture medium is prepared through the method that 4 g of glucose, 0.4 g of monopotassium phosphate, 0.2 g of magnesium sulfate, 0.1 g of zinc sulfate, 8 mg of vitamin B1, 1,000 g of corn stalk particles and 1.2 liters of water are added into every one liter of the PD culture medium; fermentation is conducted for 7 days in the environment in which the temperature is 25 DEG C and the absolute humidity is 100%. The beauveria bassiana granules have the effects of being capable of adapting to a low-humidity and high-temperature environment in a field, efficiently infecting ostrinia nubilalis and efficiently killing pests; especially, the beauveria bassiana granules have a very good infection effect on diapause-relieving ostrinia nubilalis larvae, and therefore an important significance on long-term pest resisting in a corn production area is achieved; meanwhile, the beauveria bassiana granules belong to a biological preparation and have the advantages of being environmentally friendly and safe.

Description

technical field [0001] The invention relates to the field of agricultural pest control, in particular to a biological preparation method for controlling the Asian corn borer. Background technique [0002] The Asian corn borer, Ostrinia furnacalis, belongs to Lepidoptera and Ostriniaceae. It is widely distributed in most countries in Asia and Oceania. In my country, the Asian corn borer is widely distributed from northeast to Hainan. It is the main pest in corn production in my country and seriously affects the yield and quality of corn. [0003] In order to ensure the quality and yield of corn, it is common to use pesticides to control the Asian corn borer. Conventional pesticides include chemical pesticides and biological pesticides. Biopesticide refers to the use of living organisms (fungi, bacteria, insect viruses, genetically modified organisms, natural enemies, etc.) or their metabolites (pheromones, auxins, sodium naphthalene acetate, 2,4-D, etc.) to kill agricultural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N25/12A01P7/04
CPCA01N25/12A01N63/30A01N2300/00
Inventor 汪洋洲杜茜张云月王振营李启云高月波张军
Owner JILIN ACAD OF AGRI SCI
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