Beauveria bassiana(Bals.-Criv.)Vuill and application thereof
A technology of Beauveria bassiana and bacterial agents, applied in the field of biotechnology control
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] The strain identification of implementation example 1 Beauveria bassiana
[0020] Molecular systematic identification
[0021] DNA extraction method: Quartz sand grinding CTAB extraction method (Scott et al., 2000).
[0022] Genetic markers: the translation elongation factor (tef1-alpha) gene (tef) was selected, and amplification primers: EF1T, EF2T (Bischoff et al., 2006).
[0023] PCR amplification reaction conditions: first heat at 94 degrees Celsius for 3 minutes to denature the template DNA, and then enter the following temperature cycles: denaturation at 94 degrees Celsius for 30 seconds, annealing at 50 degrees Celsius for 30 seconds, extension at 72 degrees Celsius for 45 seconds, a total of 35 cycles, and finally at 72 degrees Celsius Celsius extension for 5 minutes (Wang, 2012).
[0024] PCR product detection and sequencing: PCR products and 100bp DNA ladder (MBI Fermentas) were electrophoresed in 0.5×TBE electrophoresis buffer at 80V for 20 minutes on 2.0% ...
Embodiment 2
[0030] The indoor locust-killing characteristic measurement of implementation example 2 Beauveria bassiana:
[0031] Two treatments were set up in this experiment, namely Beauveria bassiana (Bals.-Criv.) Vuill TRCC16003 experimental group and blank control group.
[0032] The experimental treatment method is as follows: at 25 degrees Celsius, the amount of inoculated spores is 1.0×10 8 pcs / mL, put the 3rd instar locust gnats of pygmy grasshoppers into the treatment cages, and each treatment has 3 repetitions, and each group treats 30 locusts, and the dosage is 5ml / 30 heads, and spray directly on the insect cages with a small sprayer Inside. Count the number of dead insects and record the survival rate. LT was obtained by SPSS17.0 statistical analysis 50 (when lethal). The results showed that the LT of Beauveria bassiana (Bals.-Criv.) Vuill, TRCC16003 on the 3rd instar nymph of pygmy grasshoppers 50 for 4.13 days.
[0033] Table 1 Determination of indoor locust-killing ac...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com