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Beauveria bassiana(Bals.-Criv.)Vuill and application thereof

A technology of Beauveria bassiana and bacterial agents, applied in the field of biotechnology control

Active Publication Date: 2014-10-01
JIANGXI TIANREN ECOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The strain was stored in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microbial Cultures on August 13, 2013.

Method used

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  • Beauveria bassiana(Bals.-Criv.)Vuill and application thereof
  • Beauveria bassiana(Bals.-Criv.)Vuill and application thereof
  • Beauveria bassiana(Bals.-Criv.)Vuill and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The strain identification of implementation example 1 Beauveria bassiana

[0020] Molecular systematic identification

[0021] DNA extraction method: Quartz sand grinding CTAB extraction method (Scott et al., 2000).

[0022] Genetic markers: the translation elongation factor (tef1-alpha) gene (tef) was selected, and amplification primers: EF1T, EF2T (Bischoff et al., 2006).

[0023] PCR amplification reaction conditions: first heat at 94 degrees Celsius for 3 minutes to denature the template DNA, and then enter the following temperature cycles: denaturation at 94 degrees Celsius for 30 seconds, annealing at 50 degrees Celsius for 30 seconds, extension at 72 degrees Celsius for 45 seconds, a total of 35 cycles, and finally at 72 degrees Celsius Celsius extension for 5 minutes (Wang, 2012).

[0024] PCR product detection and sequencing: PCR products and 100bp DNA ladder (MBI Fermentas) were electrophoresed in 0.5×TBE electrophoresis buffer at 80V for 20 minutes on 2.0% ...

Embodiment 2

[0030] The indoor locust-killing characteristic measurement of implementation example 2 Beauveria bassiana:

[0031] Two treatments were set up in this experiment, namely Beauveria bassiana (Bals.-Criv.) Vuill TRCC16003 experimental group and blank control group.

[0032] The experimental treatment method is as follows: at 25 degrees Celsius, the amount of inoculated spores is 1.0×10 8 pcs / mL, put the 3rd instar locust gnats of pygmy grasshoppers into the treatment cages, and each treatment has 3 repetitions, and each group treats 30 locusts, and the dosage is 5ml / 30 heads, and spray directly on the insect cages with a small sprayer Inside. Count the number of dead insects and record the survival rate. LT was obtained by SPSS17.0 statistical analysis 50 (when lethal). The results showed that the LT of Beauveria bassiana (Bals.-Criv.) Vuill, TRCC16003 on the 3rd instar nymph of pygmy grasshoppers 50 for 4.13 days.

[0033] Table 1 Determination of indoor locust-killing ac...

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Abstract

The invention relates to preparation methods and applications of beauveria bassiana(Bals.-Criv.)Vuill and a bactericide thereof. Bassiana(Bals.-Criv.)Vuill especially has the effect of controlling locust pests. The accession number of bassiana(Bals.-Criv.)Vuill TRCC16003 provided by the invention in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.7998. The strain has strong spore producing capacity, has strong insecticidal activity toward three-year-old nymphs of locusts and has LT50 (lethal time of 50%) of 4.13 days. The population quantity of the locusts can be effectively controlled by utilizing the strain. Besides, bassiana(Bals.-Criv.)Vuill has good control effects on other pests such as pine moths, ostrinia nubilalis and grubs and is worthy of popularization and application.

Description

technical field [0001] The invention belongs to the field of biotechnology prevention and control, and in particular relates to the preparation method of Beauveria bassiana (Bals.-Criv.) Vuill TRCC16003 and its bacterial agent and the application in preventing and controlling plant insect pests. Background technique [0002] Locusts are insects of the family Acrididae, order Orthoptera. There are more than 10,000 species worldwide. Distributed in tropical and temperate grasslands and deserts all over the world. The mouthparts are hard, the front wings are narrow and tough, the hind wings are broad and soft, good at flying, and the hind limbs are well developed and good at jumping. Locusts are an important part of the agricultural, forestry, and animal husbandry ecosystems. Many harmful locust species can cause varying degrees of damage to agriculture, forestry, and animal husbandry. There are more than 10,000 species of locusts in the world, and about 300 species of locus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P7/04C12R1/645
Inventor 梁小文王根豪陈晓燕周立峰李肖宇马忠岩吴俊杰刘丽君李源
Owner JIANGXI TIANREN ECOLOGY
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