Application of DMNT in preparation of lepidoptera pest insecticide

An insecticide and Lepidoptera technology, applied in the application field of DMNT in the preparation of Lepidoptera pest insecticides, can solve the problems of corn grain quality decline, corn yield reduction, pesticide pollution of water resources and soil environment, etc., to achieve Effects of Reducing Reproductive Quantity, Pupation Rate and Eclosion Rate

Active Publication Date: 2020-04-28
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The gnawing ability of the larvae in the late fourth instar is greatly enhanced, and they move from the heart leaf to the outside of the plant. They mainly eat corn stalks and leaves. Farmers inflict heavy economic losses
However, most of the existing insecticides on the market are organophosphorus chemical reagents, which have the following disadvantages: (1) strong and unpleasant smell, which can cause respiratory damage to the sprayer if the sprayer inhales it, and skin damage caused by accidental contamination of the skin with the agent
(2) Pesticide residues seriously pollute water resources and soil environment, and it is difficult to degrade completely wi

Method used

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  • Application of DMNT in preparation of lepidoptera pest insecticide
  • Application of DMNT in preparation of lepidoptera pest insecticide
  • Application of DMNT in preparation of lepidoptera pest insecticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: The toxic effect of DMNT on the cruciferous crop pest Plutella xylostella.

[0026] (1) Dissolve DMNT in paraffin and dilute to a sample concentration of 75ng / μl for use.

[0027] (2) The 4-day-old Plutella xylostella was starved for 1 hour.

[0028] (3) The experiment set up a control group and an experimental group, and each group set up 6 parallel repetitions. Use a puncher to drill feeds of uniform size, add three feeds to each plate, and add 5 μl of paraffin oil (control group) or 5 μl DMNT samples (experimental group) to each feed, that is, each feed in the experimental group contains 375ng of For DMNT, 15 diamondback moth larvae were put into each group and cultured in a constant temperature incubator at 25°C.

[0029] (4) The mortality rate of Plutella xylostella, and the pupation rate and eclosion rate of Plutella xylostella in the later stage were counted respectively at 12h and 20h after treatment.

[0030] The experimental results showed that t...

Embodiment 2

[0032] Example 2: The poisonous effect of DMNT on the corn pest corn borer.

[0033] (1) Dissolve DMNT in paraffin and dilute to a sample concentration of 1 μg / μl for use.

[0034] (2) The 3rd instar and 1 day old corn borer were starved for 1 hour.

[0035] (3) The experiment set up the control group and the experimental group, with 6 parallel repetitions respectively. Use a puncher to take feeds of uniform size, add three feeds to each plate, and add 15 μl of paraffin oil (control group) or 15 μl DMNT sample (experimental group) to each feed, that is, each feed in the experimental group contains 15 μg of DMNT , put 15 3rd instar 1 day corn borer larvae into each group. The treated corn borer larvae were cultured in a constant temperature incubator at 25°C.

[0036] (4) The mortality of corn borer and the molting rate of 3rd to 4th instar larvae were counted respectively at 48h and 72h after feeding.

[0037] The experimental results showed that with the increase of feedi...

Embodiment 3

[0039] Embodiment 3: DMNT is to the poisonous effect of fall armyworm

[0040] (1) Take fresh corn leaves, and apply 100 μl of paraffin (control group) and diluted 1 μg / μl DMNT (experimental group) on the leaves evenly, that is, each leaf of the experimental group contains 100 μg of DMNT.

[0041] (2) The 3rd instar Spodoptera frugiperda larvae were starved for 1 hour.

[0042] (3) Three Spodoptera frugiperda larvae were added to the control group (paraffin) and the experimental group (DMNT), respectively, and three biological replicates were set up.

[0043] (4) Put the treated Spodoptera frugiperda into a constant temperature incubator at 25°C.

[0044] The experimental results showed that the larvae in the control group did not die, and the larvae mortality in the experimental group exceeded 60% ( Image 6 ). It shows that DMNT can effectively kill the larvae of fall armyworm.

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Abstract

The invention relates to an application of DMNT in preparation of a lepidoptera pest insecticide, which belongs to the technical field of pesticide preparation. DMNT is a natural secondary metaboliteof plants and has no damage to the plants. The prior art shows that DMNT has volatility and can attract natural enemies of pests to prey the pests. According to the invention, the DMNT is found to have a poisoning effect on three lepidoptera pests including plutella xylostella, ostrinia nubilalis and grassland spodoptera litura for the first time, the pupation rate and eclosion rate of the pests can be remarkably reduced, and the propagation quantity of the pests is reduced. The substance is easy to volatilize and metabolize and does not pollute soil and a water source, so that development ofa novel efficient pollution-free natural insecticide by taking the substance as a research object has important significance on prevention and control of crop pests.

Description

technical field [0001] The invention belongs to the technical field of pesticide manufacture, and in particular relates to the application of DMNT in the preparation of insecticides for lepidopteran pests. Background technique [0002] The common crops of cruciferous crops include various vegetables such as oilseed rape and cabbage, and the main pest is diamondback moth (plutella xylostella, PX). Plutella xylostella, also known as hanging silkworm, is one of the main pests of Lepidoptera. The first instar larvae eat leaves and form holes and nicks. When they grow into the third instar, their feeding ability is greatly enhanced. The fourth instar larvae enter the gluttony period and can The parasitic plant leaves are eaten into a web. Plutella xylostella has a short life cycle, fast reproduction speed, and strong adaptability to the environment. It can cause pest outbreaks in a short period of time and seriously endanger the growth of economic crops. [0003] Corn borer (as...

Claims

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Application Information

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IPC IPC(8): A01N27/00A01P7/04
CPCA01N27/00
Inventor 李培金陈晨王传宏陶珍陈红亿
Owner ANHUI AGRICULTURAL UNIVERSITY
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