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Bt proteins with insecticidal activity for ostrinia nubilalis and application thereof

An insecticidal activity, corn borer technology, applied in the application, insecticide, biocide and other directions

Inactive Publication Date: 2015-07-01
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In addition, at present, the Bt protein active against corn borer has been applied to the control of corn borer, resulting in the resistance of corn borer to some Bt proteins. Pests provide abundant candidate proteins, which will also be of great significance for delaying the emergence of resistance

Method used

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  • Bt proteins with insecticidal activity for ostrinia nubilalis and application thereof
  • Bt proteins with insecticidal activity for ostrinia nubilalis and application thereof
  • Bt proteins with insecticidal activity for ostrinia nubilalis and application thereof

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Experimental program
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Effect test

Embodiment 1

[0031] 1 Protein extraction

[0032] 1) Extraction of Cry1Ab, Cry1Ac, and Cry2Ab proteins

[0033] Cry1Ab, Cry1Ac, and Cry2Ab proteins were all expressed in Bacillus thuringiensis, and were extracted using 1 / 2LB liquid medium and repeated dissolving methods. For specific operations, see: "Enzyme System for Solid Culture of C. Compare" article.

[0034] 2) Extraction of Cry1Bb, Cry1Be, Cry9Aa, Cry9Eb, Cry9Ee, and Vip3A11 proteins Cry1Bb, Cry1Be, Cry9Aa, Cry9Eb, Cry9Ee, and Vip3A11 proteins were all expressed in Escherichia coli, and extracted by LB medium, broken and centrifuged, and the specific operation See: "Comparison of enzyme systems for solid cultures of C. conchiformis and Phanerolea chrysosporium". However, due to the different optimal expression conditions of each protein, through repeated exploration, it was found that: Cry1Bb had the highest expression level in the soluble fraction at 18°C ​​with a final IPTG concentration of 1.0mmol / L, and the insoluble fraction...

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Abstract

The invention provides Bt proteins with an insecticidal activity for ostrinia nubilalis and an application thereof. The proteins come from bacillus thuringiensis and can be at least one of Cry1Bb protein or Cry1Be protein. The several Bt proteins with a high toxicity to ostrinia nubilalis, provided by the invention, provide new gene sources for preparation of anti-ostrinia nubilalis transgenic crops and are capable of effectively delaying the generation of resistance.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to several Bt proteins with insecticidal activity against corn borer and their application. Background technique [0002] The corn borer (Pyrausta nubilalis) belongs to the order Lepidoptera and is the main insect pest of corn. Mainly distributed in Beijing, Northeast China, Hebei, Henan, Sichuan, Guangxi and other places. Corn sown in spring, summer and autumn in various places suffered damage to varying degrees, especially corn sown in summer. Corn borer can harm all parts of the corn plant above the ground, making the damaged part lose its function and reducing the grain yield. [0003] In addition, due to climate warming and changes in planting patterns, pests and diseases have aggravated the situation of corn borer damage, and the problem of prevention and control is urgent. However, chemical control requires a lot of manpower and material resources, so biological control is the...

Claims

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Application Information

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IPC IPC(8): C07K14/325A01N47/44A01P7/04A01H5/00
CPCA01N47/44C07K14/325C12N15/8286
Inventor 束长龙杨素娟耿丽丽张杰宋福平彭琦梁影屏
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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