Fertilizer prepared from fructus psoraleae waste and fermentation product of citrus chachiensis

Abstract

The invention discloses a fertilizer prepared from fructus psoraleae waste and a fermentation product of Citrus chachiensis Hance, and belongs to the technical field of agricultural fertilizers. The fertilizer prepared from the fermentation product of the fructus psoraleae waste and the citrus reticulata blanco mainly comprises the following raw material components in parts by mass: 30-40 parts of the fructus psoraleae waste, 90-110 parts of citrus reticulata blanco pulp, 7.52-7.92 parts of a complex microbial inoculant, 20-40 parts of an auxiliary material and 0.4 part of urea, the complex microbial inoculant comprises any one or a combination of at least two of bacillus subtilis, bacillus thuringiensis, citrobacter, bacillus cereus and trichoderma; the auxiliary materials comprise any one or a combination of at least two of rice hull powder, peanut bran, bean dregs, sawdust, wheat bran, mushroom dregs and traditional Chinese medicine residues.

Description

Technical Field

[0001] The invention relates to a fertilizer prepared from a fermentation product of psoralea corylifolia waste and Citrus aurantium. Background Art

[0002] Xinhui mandarin oranges, also known as Xinhui Guangchen mandarin oranges, tangerine peel mandarin oranges, and Xinhui big red mandarin oranges (scientific name: Tea-branch mandarin orange, Citrus reticulate 'Chachi'), are a species of citrus in the Rutaceae family, endemic to Xinhui District, Jiangmen City, Guangdong Province. As a premium variety carefully selected over 700 years of cultivation, Xinhui mandarin oranges are renowned for their dual uses of peel and flesh, serving as both medicine and food. Their peel is not only a crucial ingredient in producing authentic Xinhui tangerine peel but also possesses exceptional medicinal and edible value. However, despite the mandarin's inherent treasures, the peel's value far outweighs the flesh, leading to the significant amount of nutritious Xinhui mandarin pulp being overlooked. In fact, this pulp is rich in nutrients, such as vitamin C and dietary fiber, and possesses significant potential for resource utilization.

[0003] With the development of the Xinhui mandarin orange industry, the issue of how to deal with the remaining pulp after processing has become increasingly prominent. Currently, most Xinhui mandarin orange pulp is handled in a relatively primitive manner, mainly discarded or simply composted. This method not only wastes the valuable nutrients in the pulp but also may pollute the environment due to incomplete degradation. In particular, the pulp of tea-branch mandarin oranges, which accounts for over 60% of the total weight of the fruit, becomes a major byproduct of the tangerine peel processing. The pectin, sugars, and other substances contained in this pulp are not fully utilized, further exacerbating the problem of resource waste.

[0004] Meanwhile, the aboveground stems and leaves of the traditional Chinese medicinal herb Psoralea corylifolia are typically incinerated or discarded after the fruit is harvested. However, research has shown that these discarded stems and leaves contain a variety of potentially fertilizing ingredients, such as coumarins, flavonoids, and terpenes, which, if effectively utilized, could be transformed into a valuable agricultural resource. Unfortunately, there is currently little research on the reuse of this type of waste, and no systematic solution has been developed.

[0005] Existing technologies for converting single agricultural waste into fertilizer often suffer from nutrient imbalance and low fermentation efficiency. For example, composting only Xinhui mandarin orange pulp can lead to slow and unstable fermentation due to its high water content and low carbon-nitrogen ratio. Meanwhile, fermenting only Psoralea corylifolia stems and leaves, due to their high cellulose content, is difficult to rapidly decompose and release effective nutrients.

[0006] Given this situation, it is crucial to develop a method for combining Psoralea corylifolia waste with Citrus aurantium pulp and optimizing the fermentation process to produce a highly efficient and environmentally friendly fertilizer. This approach not only effectively recycles agricultural waste and reduces environmental pollution, but also produces a more efficient and fully decomposed organic fertilizer, providing new ideas and technical support for promoting green and sustainable agricultural development. Summary of the Invention

[0007] The purpose of the present invention is to provide a fertilizer prepared from the fermentation product of Psoralea corylifolia waste and Citrus aurantium, so as to solve the technical problems mentioned in the above background technology.

[0008] The technical solution for achieving the purpose of the present invention is:

[0009] The present invention provides a fertilizer prepared from a fermentation product of psoralea corylifolia waste and citrus aurantium, characterized in that, calculated by weight, the raw material components mainly include 30 to 40 parts by weight of psoralea corylifolia waste, 90 to 110 parts by weight of citrus aurantium pulp, 7.52 to 7.92 parts by weight of a composite bacterial agent, 20 to 40 parts by weight of auxiliary materials, and 0.4 parts by weight of urea.

[0010] Furthermore, the composite bacterial agent includes any one or a combination of at least two of Bacillus subtilis, Bacillus thuringiensis, Citrobacter rodentium, Bacillus cereus, and Trichoderma.

[0011] Furthermore, the auxiliary materials include any one or a combination of at least two of rice husk powder, peanut bran, bean dregs, wood chaff, wheat bran, mushroom residue, and traditional Chinese medicine residue.

[0012] Furthermore, the preparation steps of the fertilizer are as follows:

[0013] (1) Weigh each raw material component according to its weight;

[0014] (2) Crush the tea citrus pulp and set aside;

[0015] (3) chopping the waste of Psoralea corylifolia into small pieces and setting aside for later use;

[0016] (4) uniformly mixing the crushed citrus fruit pulp of step (2) with the chopped psoralea corylifolia waste, auxiliary materials, and urea of ​​step (3) to obtain a mixture;

[0017] (5) Add activated compound bacteria to the mixture, mix well, and then turn the mixture over once every 2 days and once every 4 days until the composting is complete to obtain fertilizer.

[0018] Furthermore, the particle size of the shredded Psoralea corylifolia waste in step (3) is 1 to 1.5 cm.

[0019] Furthermore, the activation temperature of Bacillus subtilis, Bacillus thuringiensis, Citrobacter rodentium, and Bacillus cereus in the activated composite bacterial agent is 35-37° C., and the activation time is 12-24 hours; the activation temperature of Trichoderma in the activated composite bacterial agent is 27.8-28.2° C., and the activation time is 48-60 hours.

[0020] Furthermore, the mass ratio of Bacillus subtilis, Bacillus thuringiensis, Citrobacter rodentium, Bacillus cereus and Trichoderma in the composite bacterial agent is 0-10:0-20:10-20:10-20:0-10.

[0021] Furthermore, the viable count of the Bacillus subtilis is 10 5 ~10 7 CFU / g, the number of viable bacteria of Bacillus thuringiensis is 10 6 ~10 8 CFU / g, the number of viable bacteria of Citrobacter is 10 5 ~10 7 CFU / g, the number of viable bacteria of Bacillus cereus is 10 6 ~10 8 CFU / g, the viable count of Trichoderma is 10 6 ~10 8 CFU / g.

[0022] Furthermore, the moisture content of the mixture in step (4) is 63-67%.

[0023] Furthermore, the temperature of the composting pile is maintained at 37.8-38.2°C.

[0024] By adopting the above technical solution, the present invention has the following beneficial effects:

[0025] (1) Figures 1 to 6 As shown, the fresh leaves, fresh stems, inflorescence axes and fruits of Psoralea corylifolia contain potential fertilizer ingredients such as coumarins, flavonoids and terpenes. The present invention prepares a fertilizer using fermentation products of Psoralea corylifolia waste and Citrus aurantium pulp as raw materials. By combining Citrus aurantium pulp rich in high carbon source components such as pectin and sugar with Psoralea corylifolia stems and leaves containing nitrogen sources and active substance components such as lignin and flavonoids, carbon and nitrogen complementarity between the two is achieved. In addition, the organic acids in Citrus aurantium pulp contribute to the dissolution of minerals, while the flavonoids in the Psoralea corylifolia stems and leaves can enhance the disease resistance of plants and promote a significant increase in the production of humic acid. This not only achieves waste utilization and environmental friendliness, but also improves the comprehensive fertilizer effect of the fertilizer.

[0026] (2) The fertilizer prepared by the present invention, which is composed of waste psoralea corylifolia and fermentation products of tea-branch orange, adopts a composite bacterial agent composed of any one or at least two of Bacillus subtilis, Bacillus thuringiensis, Citrobacter, Bacillus cereus and Trichoderma, and can fully and comprehensively utilize the pectin, cellulose, protein, organic matter and nitrogen, phosphorus, potassium and other components in the tea-branch orange pulp, thereby producing a biological organic fertilizer rich in nutrients; during the fermentation process, the cellulose, protein and other macromolecular substances in the material are efficiently decomposed into small molecular nutrients such as glucose and amino acids, so that the microorganisms can quickly and effectively decompose the pectin component in the citrus peel residue; at the same time, the cellulose in the waste psoralea corylifolia and the protein in the auxiliary material also help to promote the conversion of organic nitrogen, phosphorus, potassium and other nutrients into a form that can be directly absorbed and utilized by plants, significantly accelerating the compost fermentation process and effectively reducing the generation of odorous gases during the fermentation process; compared with ordinary biological organic fertilizers available on the market, the fertilizer obtained by the present invention has a higher total nutrient content and a more thorough decomposition degree, which can better meet the needs of plant growth for fast-acting nutrients. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to make the content of the present invention more clearly understood, the present invention is further described in detail below based on specific embodiments and in conjunction with the accompanying drawings, wherein

[0028] Figure 1 This is the HPLC fingerprint of the fresh leaves of Psoralea corylifolia used in the embodiments of the present invention.

[0029] Figure 2 This is the HPLC fingerprint of the fresh stem of Psoralea corylifolia used in the embodiments of the present invention.

[0030] Figure 3 This is the HPLC fingerprint of the inflorescence axis of Psoralea corylifolia used in the embodiments of the present invention.

[0031] Figure 4 This is the HPLC fingerprint of the fruit of Psoralea corylifolia used in the embodiments of the present invention.

[0032] Figure 5 This is the TLC diagram of different parts of Psoralea corylifolia at a wavelength of 254nm.

[0033] Figure 6 This is the TLC diagram of different parts of Psoralea corylifolia at a wavelength of 365nm.

[0034] The numbers in the accompanying drawings are: 1: psoralen; 2: isopsoralen; 3: psoralen; 4: isopsoralen; 5: neopsoralen isoflavone; 6: psoralen A; 7: psoralen; 8: psoralen; 9: psoralen B; 10: psoralen dihydroflavonoid methyl ether; 11: psoralen isoflavone A; 12: bakuchiol. DETAILED DESCRIPTION

[0035] In order to better understand the above technical solution, the above technical solution will be described in detail below in conjunction with specific implementation methods.

[0036] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative work shall fall within the scope of protection of the present invention.

[0037] The following examples are only used to more clearly illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention.

[0038] Bacillus subtilis ZKCC15341, Citrobacter rodentium ZKCC21142, Bacillus cereus ZKCC16563 and Trichoderma sp. ZKCC153709 were all from Beijing Zhongke Quality Inspection Biotechnology Co., Ltd., and Bacillus thuringiensis ATCC336429 was from Shanghai Lianmai Bioengineering Co., Ltd.

[0039] The culture medium for Bacillus subtilis, Bacillus thuringiensis, Citrobacter rodentium, Bacillus cereus and Trichoderma was 2% brown sugar aqueous solution. The activation temperature for Trichoderma was 28°C and the activation time was 48h. The activation temperature for Bacillus subtilis, Bacillus thuringiensis, Citrobacter rodentium and Bacillus cereus was 36°C and the activation time was 18h. Before fermentation, they were activated with culture medium respectively. After adjustment, the number of viable bacteria of Bacillus subtilis was controlled to be 10 6 CFU / g, the number of viable bacteria of Bacillus thuringiensis is 10 7 CFU / g, the number of viable bacteria of Citrobacter is 10 6 CFU / g, the number of viable bacteria of Bacillus cereus is 10 7 CFU / g, the viable count of Trichoderma is 10 7 CFU / g.

[0040] The mass ratio of Bacillus subtilis, Bacillus thuringiensis, Citrobacter rodentium, Bacillus cereus and Trichoderma in the composite bacterial agent is 5:10:10:10:5.

[0041] The auxiliary materials consist of 20 parts by mass of peanut bran, 10 parts by mass of bean dregs, and 10 parts by mass of traditional Chinese medicine residues; among them, the traditional Chinese medicine residues are taken from the pediatric Jiebiao granules produced by Good Doctor Pharmaceutical Group Co., Ltd. in Anzhou District, Mianyang City, Sichuan Province. The main raw materials are: honeysuckle, forsythia, stir-fried burdock fruit, kudzu root, schizonepeta spicate, perilla leaf, siler, dandelion, scutellaria, and bezoar; the pH of the traditional Chinese medicine residues is 7.15, the organic matter content is 57.37%, the conductivity is 3.40, the cellulose content is 11.85%, and the lignin content is 46.56%.

[0042] (Example 1)

[0043] The preparation steps of the fertilizer prepared from the fermentation product of Psoralea corylifolia waste and Citrus aurantium are as follows:

[0044] (1) Weighing and preparing the raw material components according to the following weight parts: the raw material components mainly include 30 weight parts of Psoralea corylifolia waste, 90 weight parts of Citrus aurantium pulp, 7.52 weight parts of composite bacterial agent, 20 weight parts of auxiliary materials, and 0.4 weight parts of urea;

[0045] (2) Crush the tea citrus pulp and set aside;

[0046] (3) Chop the Psoralea corylifolia waste into particles of 1 to 1.5 cm in size and set aside;

[0047] (4) uniformly mixing the crushed citrus fruit pulp of step (2) with the chopped psoralea corylifolia waste of step (3), auxiliary materials, and urea to obtain a mixture having a pH of 6.8 and a water content of 65%;

[0048] (5) Add activated composite bacteria to the mixture, mix well and then compost and ferment. Maintain the temperature of the pile at 38.2°C. Turn the material over every 2 days and every 4 days until the compost is fully decomposed to obtain fertilizer.

[0049] (Example 2)

[0050] The preparation steps of the fertilizer prepared from the fermentation product of Psoralea corylifolia waste and Citrus aurantium are as follows:

[0051] (1) Weighing and preparing the raw materials according to the following weight parts: the raw materials mainly include 35 weight parts of Psoralea corylifolia waste, 100 weight parts of Citrus aurantium pulp, 7.72 weight parts of composite bacterial agent, 30 weight parts of auxiliary materials, and 0.4 weight parts of urea;

[0052] (2) Crush the tea citrus pulp and set aside;

[0053] (3) Chop the Psoralea corylifolia waste into particles of 1 to 1.5 cm in size and set aside;

[0054] (4) uniformly mixing the crushed citrus fruit pulp of step (2) with the chopped psoralea corylifolia waste of step (3), auxiliary materials, and urea to obtain a mixture having a pH of 7.0 and a water content of 63%;

[0055] (5) Add activated composite bacteria to the mixture, mix well and then compost and ferment. Maintain the temperature of the pile at 37.8°C. Turn the material over every 2 days and every 4 days until the compost is fully decomposed to obtain fertilizer.

[0056] (Example 3)

[0057] The preparation steps of the fertilizer prepared from the fermentation product of Psoralea corylifolia waste and Citrus aurantium are as follows:

[0058] (1) Weighing and preparing the raw material components according to the following weight parts: the raw material components mainly include 40 weight parts of Psoralea corylifolia waste, 110 weight parts of Citrus aurantium pulp, 7.92 weight parts of composite bacterial agent, 40 weight parts of auxiliary materials, and 0.4 weight parts of urea;

[0059] (2) Crush the tea citrus pulp and set aside;

[0060] (3) Chop the Psoralea corylifolia waste into particles of 1 to 1.5 cm in size and set aside;

[0061] (4) uniformly mixing the crushed citrus fruit pulp of step (2) with the chopped psoralea corylifolia waste of step (3), auxiliary materials, and urea to obtain a mixture having a pH of 6.7 and a water content of 66%;

[0062] (5) Add activated composite bacteria to the mixture, mix well and then compost and ferment. Maintain the temperature of the pile at 38°C. Turn the material over every 2 days and every 4 days until the compost is fully decomposed to obtain fertilizer.

[0063] (Comparative Example 1)

[0064] The steps for preparing fertilizer are as follows:

[0065] (1) The raw material components were weighed and prepared according to the following mass parts: the raw material components mainly included 100 mass parts of tea-branch orange pulp, 7.72 mass parts of composite bacterial agent, 30 mass parts of auxiliary materials, and 0.4 mass parts of urea;

[0066] (2) Crush the tea citrus pulp and set aside;

[0067] (3) mixing the crushed citrus pulp of step (2) with auxiliary materials and urea to obtain a mixture having a pH of 6.4 and a water content of 63%;

[0068] (4) Add activated composite bacteria to the mixture, mix well and then compost and ferment. Maintain the temperature of the pile at 37.8°C. Turn the material over every 2 days and every 4 days until the compost is fully decomposed to obtain fertilizer.

[0069] (Comparative Example 2)

[0070] The steps for preparing fertilizer are as follows:

[0071] (1) Weighing and preparing the raw material components according to the following weight parts: the raw material components mainly include 35 weight parts of Psoralea corylifolia waste, 7.72 weight parts of composite bacterial agent, 30 weight parts of auxiliary materials, and 0.4 weight parts of urea;

[0072] (2) Chop the waste Psoralea corylifolia into pieces with a particle size of 1 to 1.5 cm and set aside;

[0073] (3) mixing the chopped Psoralea corylifolia waste, auxiliary materials, and urea uniformly to obtain a mixture having a pH of 7.2 and a moisture content of 63%;

[0074] (4) Add activated composite bacteria to the mixture, mix well and then compost and ferment. Maintain the temperature of the pile at 37.8°C. Turn the material over every 2 days and every 4 days until the compost is fully decomposed to obtain fertilizer.

[0075] (Effect Example)

[0076] With reference to the bio-organic fertilizer standard of the Agricultural Industry Standard of the People's Republic of China NY 525-2012, conventional nutrient indicators of organic fertilizer were determined; humus in bio-organic fertilizer was determined according to the humus determination method in GB 7858-1987; the test results of the fertilizers of Examples 1 to 3 and Comparative Examples 1 to 2 are as follows:

[0077] The fertilizer of Example 1 has a nitrogen content of 4.93%, a phosphorus content of 1.04%, a potassium content of 3.70%, a total nutrient content of 9.67%, an organic matter content of 72.16%, and a humic acid content of 25.87%.

[0078] The fertilizer of Example 2 has a nitrogen content of 5.12%, a phosphorus content of 1.06%, a potassium content of 3.72%, a total nutrient content of 9.90%, an organic matter content of 72.94%, and a humic acid content of 26.54%.

[0079] The fertilizer of Example 3 has a nitrogen content of 5.06%, a phosphorus content of 1.05%, a potassium content of 3.70%, a total nutrient content of 9.81%, an organic matter content of 72.38%, and a humic acid content of 26.34%.

[0080] The fertilizer of Comparative Example 1 has a nitrogen content of 2.05%, a phosphorus content of 0.45%, a potassium content of 2.43%, a total nutrient content of 4.93%, an organic matter content of 61.55%, and a humic acid content of 17.40%.

[0081] The fertilizer of Comparative Example 2 has a nitrogen content of 3.61%, a phosphorus content of 1.03%, a potassium content of 1.53%, a total nutrient content of 6.17%, an organic matter content of 68.35%, and a humic acid content of 21.37%.

[0082] The technical indicators of the fertilizers obtained in Examples 1 to 3 all meet the requirements of the national organic fertilizer standards and the requirements of the "Agricultural Industry Standards of the People's Republic of China", especially the organic matter content can reach more than 72.00%. Compared with Comparative Example 1 in which only tea-branch orange pulp is used as a raw material and supplemented with a composite bacterial agent, auxiliary materials, and urea, and Comparative Example 2 in which only psoralea corylifolia waste is used as a raw material and supplemented with a composite bacterial agent, auxiliary materials, and urea, the organic matter content is higher, the humic acid content is higher, and the fermentation and decomposition are more thorough, indicating that when psoralea corylifolia waste and tea-branch orange pulp are used to prepare fertilizer together, they have the effect of promoting each other's decomposition, making the fertilizer more thoroughly decomposed, and having better nutritional value and organic fertilizer effect.

[0083] Seed germination rate determination: Refer to the seed germination rate determination method in GB 7858-1987; weigh 10.0 g of the fertilizers prepared in the Examples and Comparative Examples and dissolve them in 100 mL of distilled water. Shake at 25°C for 30 minutes, then filter. Add 10 mL of the filtrate to a clean, 150 mm diameter Petri dish lined with two layers of filter paper. Then, sow pakchoy seeds evenly on the filter paper. Incubate 50 seeds with each fertilizer, setting up three replicates and averaging the results. A control group received distilled water and incubated in a 30°C incubator in the dark for 60 hours. Germination was determined when the radicle reached the same length as the seed and the plumule reached half the seed length.

[0084] Table 1 below shows the test results of seed germination rates corresponding to the fertilizers prepared in Examples and Comparative Examples:

[0085] Table 1

[0086] Germination rate (%) control group 98.67 Example 1 99.24 Example 2 99.33 Example 3 99.30 Comparative Example 1 83.32 Comparative Example 2 95.32

[0087] Undecomposed compost produces phytotoxic substances that inhibit plant growth, while decomposed compost promotes growth. Therefore, the biomass of plant growth can indicate the maturity of compost. The germination index (GI) reflects the toxicity of compost products to plants and is an indicator of compost maturity. Considering that compost products are ultimately used in agricultural production, plant growth tests should be the final and most convincing method for evaluating compost maturity. It is generally accepted that when the GI is greater than 50%, the content of toxic substances in the compost can be considered to be reduced to a level that plants can tolerate; when the GI is ≥85%, the compost is considered fully mature.

[0088] As shown in Table 1 above, the fertilizers prepared in Examples 1 to 3 were fully decomposed and promoted seed germination, while the fertilizer obtained in Comparative Example 1 using only tea-branch citrus pulp and composite microbial agent, auxiliary materials, and urea was not fully decomposed; the fertilizer obtained in Comparative Example 2 using psoralea corylifolia waste, composite microbial agent, auxiliary materials, and urea was fully decomposed.

[0089] In summary, the fertilizer made from Psoralea corylifolia waste and Citrus aurantium pulp, supplemented by composite microbial agents, auxiliary materials, and urea, combines Citrus aurantium pulp rich in high carbon sources such as pectin and sugars with Psoralea corylifolia stems and leaves containing nitrogen sources and active substances such as lignin and flavonoids, and has a higher total nutrient content and more thorough decomposition, which can better meet the plant growth demand for fast-acting nutrients.

[0090] The specific embodiments described above further illustrate the objectives, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above are only specific embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A fertilizer prepared from a fermentation product of Psoralea corylifolia waste and Citrus aurantium, characterized in that: Calculated by mass, the raw material components mainly include 30 to 40 parts by mass of Psoralea corylifolia waste, 90 to 110 parts by mass of Citrus aurantium pulp, 7.52 to 7.92 parts by mass of composite bacterial agent, 20 to 40 parts by mass of auxiliary materials, and 0.4 parts by mass of urea.

2. The fertilizer prepared from the fermentation product of Psoralea corylifolia waste and Citrus aurantium according to claim 1, characterized in that: The composite bacterial agent comprises any one of Bacillus subtilis, Bacillus thuringiensis, Citrobacter rodentium, Bacillus cereus and Trichoderma or a combination of at least two of them.

3. The fertilizer prepared from the fermentation product of Psoralea corylifolia waste and Citrus aurantium according to claim 1, characterized in that: The auxiliary materials include any one of rice husk powder, peanut bran, bean dregs, wood chaff, wheat bran, fungus residue, and traditional Chinese medicine residue, or a combination of at least two of the above.

4. The fertilizer prepared from the fermentation product of Psoralea corylifolia waste and Citrus aurantium according to any one of claims 1 to 3, characterized in that: The preparation steps of the fertilizer are as follows: (1) Weigh each raw material component according to its weight; (2) Crush the tea citrus pulp and set aside; (3) chopping the waste of Psoralea corylifolia into small pieces and setting aside for later use; (4) uniformly mixing the crushed citrus fruit pulp of step (2) with the chopped psoralea corylifolia waste, auxiliary materials, and urea of ​​step (3) to obtain a mixture; (5) Add activated compound bacteria to the mixture, mix well, and then turn the mixture over once every 2 days and once every 4 days until the composting is complete to obtain fertilizer.

5. The fertilizer prepared from the fermentation product of Psoralea corylifolia waste and Citrus aurantium according to any one of claim 4, characterized in that: The particle size of the shredded Psoralea corylifolia waste in step (3) is 1 to 1.5 cm.

6. The fertilizer prepared from the fermentation product of Psoralea corylifolia waste and Citrus aurantium according to any one of claim 4, characterized in that: The activation temperature of Bacillus subtilis, Bacillus thuringiensis, Citrobacter rodi and Bacillus cereus in the activated composite bacterial agent is 35-37° C., and the activation time is 12-24 hours; the activation temperature of Trichoderma in the activated composite bacterial agent is 27.8-28.2° C., and the activation time is 48-60 hours.

7. The fertilizer prepared from the fermentation product of Psoralea corylifolia waste and Citrus aurantium according to any one of claim 4, characterized in that: The mass ratio of Bacillus subtilis, Bacillus thuringiensis, Citrobacter rodi, Bacillus cereus and Trichoderma in the composite bacterial agent is 0-10:0-20:10-20:10-20:0-10.

8. The fertilizer prepared from the fermentation product of Psoralea corylifolia waste and Citrus aurantium according to any one of claim 7, characterized in that: The viable count of the Bacillus subtilis is 10 5 ~10 7 CFU / g, the number of viable bacteria of Bacillus thuringiensis is 10 6 ~10 8 CFU / g, the number of viable bacteria of Citrobacter is 10 5 ~10 7 CFU / g, the number of viable bacteria of Bacillus cereus is 10 6 ~10 8 CFU / g, the viable count of Trichoderma is 10 6 ~10 8 CFU / g.

9. The fertilizer prepared from the fermentation product of Psoralea corylifolia waste and Citrus aurantium according to any one of claim 4, characterized in that: The moisture content of the mixture in step (4) is 63-67%.

10. The fertilizer prepared from the fermentation product of Psoralea corylifolia waste and Citrus aurantium according to any one of claim 4, characterized in that: The temperature of the composting pile is maintained at 37.8-38.2°C.

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