Application of compound UNC1999 in preparation of insecticide
A technology for preparing insecticides and compounds, applied in the field of pesticides, can solve problems that have not been reported, and achieve remarkable results
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Embodiment 1
[0023] Determination of the inhibitory activity of compound UNC1999 on the biosynthesis of juvenile hormone in pests.
[0024] 1. Test method:
[0025] This example adopts the juvenile hormone GC-MS / MS assay method (Journal of Chromatography A, 2018, 1538:67-74) to determine the effect of compound UNC1999 on Manduca sexta, Spodoptera litura, cotton bollworm, diamondback moth, and beet armyworm. and the inhibition of corn borer juvenile hormone biosynthesis, the specific operation is as follows:
[0026] After anesthetizing the test insects with ice, place them on a wax plate, take out the pharyngeal body (CA) by microdissection, and remove the surrounding connective tissue in M199 for biological testing. Place CA in 100 μL of M199 containing 20 nM internal standard citronellol and different concentrations of inhibitors (UNC1999), at room temperature, with slight shaking, and incubate in the dark for 3 hours; then extract with 200 μL of n-hexane, take 150 μL of the upper organ...
Embodiment 2
[0033] Determination of UNC1999 stomach toxicity activity to pests.
[0034] 1. Test method:
[0035] The newly hatched Lepidoptera pest larvae were fed on feed supplemented with different concentrations of UNC1999, and the feed was changed once a day. The corresponding weight of solvent (5 vol% dimethyl sulfoxide aqueous solution, ie DMSO aqueous solution) was added to the feed in the control group, and the rest of the conditions were consistent with those of the experimental group. Each experimental group contained 10 repeated experiments, and 10 larvae were set in each experiment. From the application of the pesticide, the number of dead worms was recorded every day until the larvae stopped eating and pupated, and finally the mortality rate was recorded.
[0036] 2. Results analysis and discussion:
[0037] The results of compound UNC1999's gastric toxicity to pests are shown in Table 2.
[0038] Table 2 Compound UNC1999 has stomach toxicity activity (mortality) to pest...
Embodiment 3
[0042] UNC1999 Determination of the ovicidal activity of pests.
[0043] 1. Test method:
[0044] With reference to the national standard method ("Pesticide Indoor Bioassay Test Guidelines Insecticides Part 5: Ovicidal Activity Test Impregnation Method" NY / T1154.5-2006), six kinds of UNC1999 in Example 1 and Example 2 were measured The ovicidal activity of Lepidoptera pests, the specific method is as follows:
[0045] 1) Get the newly produced ovum of six kinds of Lepidoptera pests, and test them after being sterilized by 10vol% formaldehyde solution;
[0046] 2) Put 100 sterilized insect eggs into each watch glass. In order to simulate the actual situation in the field, the spraying method was used in the experiment to make different concentrations of pesticides contact the eggs without completely submerging the eggs in the liquid medicine. In , 5 repeated experiments were set for each drug treatment condition (concentration). In the blank control experiment, an equal amou...
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