Application of forsythia suspensa extract in prevention and treatment of agricultural pests

Abstract

The invention provides application of forsythia suspensa extract in prevention and treatment of agricultural pests. The forsythia suspensa extract is an ethanol extract and can be used for preventingand treating agricultural pests such as armyworms, ostrinia nubilalis and grassland spodoptera litura and is environmentally friendly.

Description

technical field

[0001] The invention belongs to the field of botanical pesticides, and in particular relates to the use of a forsythia extract for preventing and controlling agricultural pests. Background technique

[0002] Forsythia is a plant of Oleaceae, and its fruit is a commonly used Chinese medicinal material. Forsythia mainly contains phenylethanol glycosides, lignans and their glycosides, pentacyclic triterpenes, volatile oils and other compounds. Pharmacological studies believe that it has antibacterial, anti-inflammatory, antipyretic, antiemetic, diuretic and cardiotonic effects, anti-liver damage, analgesic, anti-viral, and blood pressure-lowering effects.

[0003] Forsythia has been deeply studied as a traditional Chinese medicine, but less research has been done on its agricultural application. In agriculture, the research on the activity of Forsythia extract mainly focuses on the field of sterilization. For example, Forsythia extract has a good inhibitory eff...

Examples

Embodiment 1

[0017] The preparation of embodiment 1 forsythia extract

[0018] Forsythia fruit and forsythia leaves were dried in a constant temperature oven at 75°C until constant weight, crushed and sieved, and 70% ethanol was added at a ratio of 1:15 (g / mL) to liquid, and refluxed at 80°C for 3 hours. Extract three times, combine the filtrate after filtering, and concentrate the filtrate by rotary evaporation to obtain the ethanol extract of forsythia fruit and forsythia leaf. Take the ethanol extract of forsythia fruit and forsythia leaf and dilute with acetone to make 1g / mL for later use.

Embodiment 2

[0019] Example 2 Determination of Contact Activity of Forsythia Extract to Armyworm

[0020] Immersion method: Dilute the sample extract prepared in Example 1 with water successively 100 times, 20 times, 10 times, 5 times, select 3rd instar armyworm larvae of the same size in the immersion tube, put them into the diluent and soak for 10s Take it out, blot the excess medicinal solution with absorbent paper, place it in a petri dish with fresh corn leaves, repeat each treatment 3 times, and set 10 test insects for each repetition, and set a blank control. Place the petri dish in an incubator at 28°C for cultivation, investigate the death of the test insects after 24 hours, and calculate the death rate.

[0021] Table 1 Contact activity of forsythia extract on third instar larvae of armyworm

[0022] deal with Number of test insects / head Number of dead insects / head mortality rate / % Corrected Mortality % 100 times dilution 30 1 3.33 3.33 20 times d...

Embodiment 3

[0024] Example 3 Determination of Stomach Toxicity Activity of Forsythia Extract to Corn Borer

[0025] Toxic leaf disc method: the sample extract prepared in Example 1 was diluted with water 100 times, 20 times, 10 times, and 5 times in sequence. Make a circular disc with a diameter of 15 mm from fresh corn leaves that have not used chemical pesticides, soak the disc in the liquid medicine for 3 seconds and take it out. After the solvent evaporates, sandwich it between two untreated discs with the same diameter Make poisonous leaf discs between them. 6 discs with poisonous leaves were placed equidistantly on each petri dish, fed with the 3rd instar larvae of the corn borer starved for 4 hours, and a blank control was set, with 20 larvae for each treatment, and then placed in an artificial climate box to continue feeding, repeated 3 times. Check and record the number of dead corn borer larvae at 48 hours, and calculate the corrected death rate.

[0026] Table 2 Stomach toxic...

technical field

[0001] The invention belongs to the field of botanical pesticides, and in particular relates to the use of a forsythia extract for preventing and controlling agricultural pests. Background technique

[0002] Forsythia is a plant of Oleaceae, and its fruit is a commonly used Chinese medicinal material. Forsythia mainly contains phenylethanol glycosides, lignans and their glycosides, pentacyclic triterpenes, volatile oils and other compounds. Pharmacological studies believe that it has antibacterial, anti-inflammatory, antipyretic, antiemetic, diuretic and cardiotonic effects, anti-liver damage, analgesic, anti-viral, and blood pressure-lowering effects.

[0003] Forsythia has been deeply studied as a traditional Chinese medicine, but less research has been done on its agricultural application. In agriculture, the research on the activity of Forsythia extract mainly focuses on the field of sterilization. For example, Forsythia extract has a good inhibitory eff...

Examples

Embodiment 1

[0017] The preparation of embodiment 1 forsythia extract

[0018] Forsythia fruit and forsythia leaves were dried in a constant temperature oven at 75°C until constant weight, crushed and sieved, and 70% ethanol was added at a ratio of 1:15 (g / mL) to liquid, and refluxed at 80°C for 3 hours. Extract three times, combine the filtrate after filtering, and concentrate the filtrate by rotary evaporation to obtain the ethanol extract of forsythia fruit and forsythia leaf. Take the ethanol extract of forsythia fruit and forsythia leaf and dilute with acetone to make 1g / mL for later use.

Embodiment 2

[0019] Example 2 Determination of Contact Activity of Forsythia Extract to Armyworm

[0020] Immersion method: Dilute the sample extract prepared in Example 1 with water successively 100 times, 20 times, 10 times, 5 times, select 3rd instar armyworm larvae of the same size in the immersion tube, put them into the diluent and soak for 10s Take it out, blot the excess medicinal solution with absorbent paper, place it in a petri dish with fresh corn leaves, repeat each treatment 3 times, and set 10 test insects for each repetition, and set a blank control. Place the petri dish in an incubator at 28°C for cultivation, investigate the death of the test insects after 24 hours, and calculate the death rate.

[0021] Table 1 Contact activity of forsythia extract on third instar larvae of armyworm

[0022] deal with Number of test insects / head Number of dead insects / head mortality rate / % Corrected Mortality % 100 times dilution 30 1 3.33 3.33 20 times d...

Embodiment 3

[0024] Example 3 Determination of Stomach Toxicity Activity of Forsythia Extract to Corn Borer

[0025] Toxic leaf disc method: the sample extract prepared in Example 1 was diluted with water 100 times, 20 times, 10 times, and 5 times in sequence. Make a circular disc with a diameter of 15 mm from fresh corn leaves that have not used chemical pesticides, soak the disc in the liquid medicine for 3 seconds and take it out. After the solvent evaporates, sandwich it between two untreated discs with the same diameter Make poisonous leaf discs between them. 6 discs with poisonous leaves were placed equidistantly on each petri dish, fed with the 3rd instar larvae of the corn borer starved for 4 hours, and a blank control was set, with 20 larvae for each treatment, and then placed in an artificial climate box to continue feeding, repeated 3 times. Check and record the number of dead corn borer larvae at 48 hours, and calculate the corrected death rate.

[0026] Table 2 Stomach toxic...

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