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Biological prevention and control method for ostrinia nubilalis

A technology for the purpose of corn borer, which is applied in the field of biological control of corn borer, and can solve the problems of increased drug resistance, environmental pollution, and difficult control

Active Publication Date: 2018-09-28
HANGZHOU FUYANG GAOBO INFORMATION TECH SERVICE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are many types of insecticides for the control of corn borer, but mainly chemical pesticides have been used for many years. Pyrethrin and organophosphorus insecticides have been used for prevention and control. The increase in the number of sprays increases the resistance to insecticides, making the prevention and control difficult, and at the same time brings Environmental Pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The biological control method of corn borer comprises the following steps: adding the compound bacterial agent to 10 times the weight of water, stirring evenly, and then mixing well with 20 mesh fine sand according to the mass ratio of 1:50 to obtain medicinal sand, according to the weight of each plant of corn Use 2g of medicated sand to prevent and control.

[0036] The composite microbial agent is prepared according to the following steps:

[0037] Step 1) Prepare the culture medium of Metarhizium anisopliae: the seed solution of Metarhizium anisopliae (1×10 8cfu / ml) according to the inoculum amount of 10% volume ratio was inserted into the expansion medium, and cultured at 28°C for 36 hours to obtain the culture medium of Metarhizium anisopliae; the formula of the expansion medium was: glucose 20g / L, yeast extract 10g , ammonium sulfate 5g / L, potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.1g / L, ferrous sulfate 0.1g...

Embodiment 2

[0044] The biological control method of corn borer, it comprises the following steps: add compound bacterial agent to the water of 20 times of weight, stir evenly, then mix well with 30 mesh fine sand according to the mass ratio of 1:80 to obtain medicinal sand, according to the weight ratio of each corn Control with 2.5g of medicinal sand.

[0045] The composite microbial agent is prepared according to the following steps:

[0046] Step 1) Prepare the culture medium of Metarhizium anisopliae: the seed solution of Metarhizium anisopliae (1×10 8 cfu / ml) according to the inoculum amount of 10% volume ratio was inserted into the expansion medium, and cultured at 28°C for 36 hours to obtain the culture medium of Metarhizium anisopliae; the formula of the expansion medium was: glucose 20g / L, yeast extract 10g , ammonium sulfate 5g / L, potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.1g / L, ferrous sulfate 0.1g / L;

[0047] Step 2) Pre...

Embodiment 3

[0053] Test environment and conditions

[0054] The site was selected as an agricultural experimental field in Fuyang District. The soil fertility was medium, the terrain was flat, the irrigation was convenient, and the fertilizer and water conditions were consistent; the corn planting time was March 26, and the seedling density was 3300 plants / mu; the cultivation level and management methods were consistent with those of the local area. unanimous.

[0055] The group setting of the compound bacterial agent: Group 1: The compound bacterial agent adopts Example 1; Group 2: only adopts the culture solution of Metarhizium anisopliae and Bacillus thuringiensis, and the rest are the same as in Example 1; Group 3: Only use the culture solution of Scarab anisopliae Bacillus thuringiensis culture fluid and Trichoderma viride-Bacillus subtilis mixed culture fluid, all the other are with embodiment 1; Group 4: only adopt Bacillus thuringiensis culture fluid and Trichoderma viride-Bacillu...

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PUM

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Abstract

The invention belongs to the technical field of biology, and discloses a biological prevention and control method for ostrinia nubilalis. The method comprises the following steps: adding a composite microbial agent into 10-20 times by weight of water, performing uniform stirring, performing uniform mixing with 20-30-mesh fine sand according to a mass ratio of 1:(50-100) to obtain pesticide sand, and spraying 2-3 g of the pesticide sand to per plant of corn for prevention and control. Compared with a conventional chemical method, the biological prevention and control method provided by the invention has the effect persistence and high control effect which can reduce pesticide application frequency, can effectively control pests, delay drug resistance of the pests, ensure safety requirementsof corn production, and protect the ecological environment; and the method is green and environmentally friendly, and has broad market application prospects.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a biological control method for corn borer. Background technique [0002] Corn borer, also known as corn borer, belongs to Lepidoptera, Boreridae. There are two types of corn borer in my country, Asian corn borer and European corn borer. They mainly damage corn, sorghum, millet, etc., and can also damage cotton, sugar cane, and cannabis , sunflower, rice, sugar beet, sugarcane beans and other crops are worldwide pests. [0003] The annual generation number of corn borer in my country varies with latitude, and 1-7 generations can occur in a year. The occurrence of each generation and each stage varies from place to place. There are also slight differences in the same occurrence area due to annual temperature changes. Normally, the eggs of the first-generation corn borer are at the heart leaf stage of spring corn in the 1st to 3rd generation areas, and the larvae-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00C12N1/20C12N1/14C12R1/125C12R1/07C12R1/885C12R1/645
CPCA01G13/00C12N1/14C12N1/20
Inventor 叶虹鲁建刚来银坤黄志芳朱利萍
Owner HANGZHOU FUYANG GAOBO INFORMATION TECH SERVICE
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