HPLC fingerprint spectrum measurement method for standard double-harmonizing decoction

A standard fingerprint and fingerprint technology, applied in the field of traditional Chinese medicine, can solve the problems of bitter taste and large amount of soup, affecting clinical efficacy, difficult to unify standards, etc., and achieve the effect of ensuring curative effect, convenience and mastery

Active Publication Date: 2017-03-15
天津同仁堂集团股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the shortcomings of deployment, carrying, temporary decoction, and long-term storage, it is prone to mildew and deterioration, the soup tastes bitter a

Method used

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  • HPLC fingerprint spectrum measurement method for standard double-harmonizing decoction
  • HPLC fingerprint spectrum measurement method for standard double-harmonizing decoction
  • HPLC fingerprint spectrum measurement method for standard double-harmonizing decoction

Examples

Experimental program
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Embodiment 1

[0059] The HPLC fingerprint spectrum assay method of embodiment one Shuanghe decoction standard decoction

[0060] 1.1 Instruments and reagents

[0061] instrument:

[0062]

[0063] Reagent:

[0064]

[0065] 1.2 Sample preparation:

[0066] 1.2.1 Preparation of samples and sample solutions for testing

[0067] (1) Preparation of samples: Weigh nine medicinal materials according to the prescription ratio, crush them, and sieve them through a 10-40 mesh sieve to obtain coarse powder; take three times the prescription amount of coarse powder into a fully automatic decoction pot, accurately measure 900mL pure water and pour Put it in, soak for 30min, decoct for 75min, stir three times during decoction, filter it with 300 mesh filter cloth while it is hot, the volume of filtrate is about 500mL, mix the filtrate evenly, take one-third of it and dilute it into a 250mL volumetric flask with pure water It is the stock solution of the standard soup, and it is for later use. ...

Embodiment 2

[0136] The quality control method of embodiment two Shuanghetang standard granules

[0137] A quality control method of Shuanghe Decoction standard granules, comprising the following steps:

[0138] (1) prepare Shuanghe decoction standard soup according to the method in 1.2.1 (1) in embodiment one, adopt the assay method in embodiment one to set up the standard fingerprint of Shuanghe decoction standard soup;

[0139] (2) Get the Shuanghetang standard granules to be checked, and obtain fingerprints according to the assay method in Example 1;

[0140] (3) Compare the fingerprints obtained in step (2) with the standard fingerprints obtained in step (1), if they meet, they are qualified products, and if they do not meet, they are unqualified products.

[0141] Preparation of the test sample solution of Shuanghetang standard granules: Take the Shuanghetang standard granules under the item of difference in filling volume, grind them finely, take about 5g, accurately weigh them, pu...

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Abstract

The invention provides a HPLC fingerprint spectrum measurement method for standard double-harmonizing decoction, belonging to the technical field of traditional Chinese medicine. The method comprises the following steps: (1) preparing a test sample solution; (2) preparing a mixed reference solution; (3) preparing a single medicine negative reference solution; (4) precisely absorbing the test sample solution, the mixed reference solution and the single medicine negative reference solution respectively and injecting into a high performance liquid chromatograph and measuring to obtain the liquid chromatogram of the test sample solution, the mixed reference solution and the single medicine negative reference solution; and (5) performing data matching on the liquid chromatogram of the test sample solution, the mixed reference solution and the single medicine negative reference solution by use of the traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system formulated by the Chinese committee of pharmacopeia to obtain a standard fingerprint spectrum. According to the invention, the quality of standard granules of double-harmonizing decoction is effectively controlled, the curative effect of the drug is guaranteed, and more formal quality control is realized on the classical famous prescription.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to an HPLC fingerprint determination method of Shuanghe decoction standard decoction. Background technique [0002] Shuanghe Decoction was first recorded in Volume 5 of "Taiping Huimin Hejiju Prescriptions" in the Southern Song Dynasty. It is a new prescription for Baoqing to treat all deficiency. The original prescription is recorded as white peony (seven and a half), angelica (washed, soaked in wine), astragalus (honey-roasted), Chuanxiong, rehmannia glutinosa (cleaned, wine-steamed, three liang each), licorice (broiled), cinnamon (removed Skin, no fire, two and a half cents each). The above is fine powder, two qian per serving, one and a half cups of water, three slices of ginger, one jujube, decocted for six minutes, hollowed out, and taken before eating. Treat men and women with five fatigues, six extremes, and seven injuries, with deficiency of heart an...

Claims

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Application Information

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IPC IPC(8): G01N30/74G01N30/86G01N30/06
CPCG01N30/06G01N30/74G01N30/8686
Inventor 张彦森高桂琴
Owner 天津同仁堂集团股份有限公司
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