The invention discloses a clarifying process and a quality control method of a medicinal composition with the function of repairing skin barrier. The medicine is composed of 6 medicinal materials including radix cynanchi atrati, pilea sinofasciata, folium ginkgo, radix seu herba polygalae fallacis, Adinandra nitida Merr.ex H.L.Li and gemma terminalis anoectochili roxburghii; for the specific clarifying process, a II type ZTC 1+1 natural clarifying agent is adopted for clarifying an extracting solution of the medicinal composition; for the specific quality control method, a thin-layer chromatography method is adopted for carrying out thin-layer identification on radix cynanchi atrati and folium ginkgo in the medicinal composition, and meanwhile, the contents of Hernandulcin, tenuigenin, camelliaside A and kinsenoside in the medicinal composition are measured by adopting a high performance liquid chromatography method. The clarifying process and the quality control method adopted by theinvention have the advantages that the equipment investment is small, the operation process is simple, the defects that in the traditional alcohol precipitation method, the alcohol consumption amountis large, the alcohol recovery is complicated, the measurement for various ingredients of the medicine is complicated, etc., are overcome, the inherent quality, curative effects and stability of the composition are quickly and effectively guaranteed, and the controllability of the quality standard of the medicinal composition is improved.